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The map label for this gene is gsiB [H]

Identifier: 23100526

GI number: 23100526

Start: 3189081

End: 3190706

Strand: Reverse

Name: gsiB [H]

Synonym: OB3071

Alternate gene names: 23100526

Gene position: 3190706-3189081 (Counterclockwise)

Preceding gene: 23100529

Following gene: 23100521

Centisome position: 87.89

GC content: 38.31

Gene sequence:

>1626_bases
GTGAAATTGTCAAAAAAGTCTTTATTATTATTAGTTTTTGGTTTACTTCTAAGTGTTGTACTAGTAGCATGTGCTAGTGA
ACCTGATGAAGGTGGTAACACAGATTCTTCTGGTGATTCAGATGCAGACTCTGAAGAATCTACAGAAGGCGGCGAGGGTG
GAGAGCTTGTTATTGCAACTCTATCAGATGCCGTATCGTTGGATCCTGCTGGTTCCAATGACGTACCGTCTAGTAACGTG
CAAGCAAACATATTTGAATCTTTAACAAAACAAGATGCAAATATGGAAGTACAACCAAGTCTGGCAACAGAATGGGAAGC
AATTGATGATACTACATGGGAATTCAAGCTTCGTGAAGGTGTTAAATTCCATGATGGATCAGACTTTAACGCAGAAGTGG
TTAAAGCGAATATTGAACGTATCTTAGATCCAGATGTAGGATCTCCACGTTTATTCTTATATGAAATGATTGAAGAAATT
GAAGTTGTAGATGATTATACAATTCGTTTTACAACAGAGTTTCCATTTTCACCACTTCCAGCTCACTTAGCGCATAATGG
TGGTGCAATGGTATCTCTAGATGTAATTAATGAAGACTATGCAGCTATGGAAAATGGAGAAGATCCAGGTAGTGTTATTA
ATAATAATCCTGTTGGAACAGGATTCTTCAAATTTGATAAATGGGATCCAGGTTCACAGATTCGCTTAGTTAAGAATGAA
GAATATTGGGATGAGCCTGCAAAACTTGATTCTGTAGTATTTAAGGTAGTTACTGAAGGTCTTACACGAGTAGCAGAATT
ACAAACTGGTGACTCACATATTTCTGATCCACTTAGTCCGAATGACGTAGAGCAAGTGGAAGCGATGGATGGCGTTAGTG
TAACACAACAAGGTAGTGTAAGTTTATCTTATGTTGGATTTAACATGGAAAAAGAGCCTTTCGATGATCCGAATGTACGT
AAGGCAATCAATATGGCAATTGATAAGAGCCAAATCATTGATGGTATTTATAATGGAGTAGGTACTCCAGCAATTGGGCC
TTTAGCTCCAGATGTATTTGGTTATGATGAAAATGCTCCCGGATTAGAATATGATCCTGAACAGGCAAAAGAATTGCTTG
CAGAAGCAGGATTAGAGGATGGCTTCTCAACTACACTTTGGACAAACGATAGTCAAGAGCGTGTGGATGCTGCAACAAAT
ATCCAAGCTCAATTAAAAGAATTCAATATTGATGTAGATGTACAAGTTCTTGAGTGGGGTGCGTATTTAGAGCAAACTGC
ACAAGGTGAGCATGATATGTTTGTATTAGGTTGGTCTACTGTTACTGGTGATGCGGATTATGGTTTATATGCATTGTTCC
ATTCGAATAATGTAGGAGATGCTGGTAACCGTACGTTTACAAAAGATGAAGAGTTAGATGCACTTCTAGATGAAGCGCGT
CAAGAGCCAGATCCAGAGACTCGTCAACAACTTTATAGCGAAATTCAAGTGAAATTAACTGAGGTAGCTCCGATGCTTTA
TATTCACCACCAAGACTTCTTACTTGGTGTAAGTGATAAAGTACAAGGCTTAACTCAATTACCAACACAAATTCTTCAGT
TGAAAGACGTAACGTTAGAACAATAA

Upstream 100 bases:

>100_bases
ATTATATCATTGAAATTTTTTAAAAAATAGATTAATATATAATAGACAGTTTTCCGAAATAAAAAAAGATTTTAAATTAG
CAACTTAGGGGGGATTTTAC

Downstream 100 bases:

>100_bases
GCTAGAATTAAAGAGAGCCCTTGTACGTTAACTCGTACAAGGGCTCTTTTTAATGTGCAAAAAGAAATTTCATCTATTAG
GATAGGCTTATAAAAACTAA

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKLSKKSLLLLVFGLLLSVVLVACASEPDEGGNTDSSGDSDADSEESTEGGEGGELVIATLSDAVSLDPAGSNDVPSSNV
QANIFESLTKQDANMEVQPSLATEWEAIDDTTWEFKLREGVKFHDGSDFNAEVVKANIERILDPDVGSPRLFLYEMIEEI
EVVDDYTIRFTTEFPFSPLPAHLAHNGGAMVSLDVINEDYAAMENGEDPGSVINNNPVGTGFFKFDKWDPGSQIRLVKNE
EYWDEPAKLDSVVFKVVTEGLTRVAELQTGDSHISDPLSPNDVEQVEAMDGVSVTQQGSVSLSYVGFNMEKEPFDDPNVR
KAINMAIDKSQIIDGIYNGVGTPAIGPLAPDVFGYDENAPGLEYDPEQAKELLAEAGLEDGFSTTLWTNDSQERVDAATN
IQAQLKEFNIDVDVQVLEWGAYLEQTAQGEHDMFVLGWSTVTGDADYGLYALFHSNNVGDAGNRTFTKDEELDALLDEAR
QEPDPETRQQLYSEIQVKLTEVAPMLYIHHQDFLLGVSDKVQGLTQLPTQILQLKDVTLEQ

Sequences:

>Translated_541_residues
MKLSKKSLLLLVFGLLLSVVLVACASEPDEGGNTDSSGDSDADSEESTEGGEGGELVIATLSDAVSLDPAGSNDVPSSNV
QANIFESLTKQDANMEVQPSLATEWEAIDDTTWEFKLREGVKFHDGSDFNAEVVKANIERILDPDVGSPRLFLYEMIEEI
EVVDDYTIRFTTEFPFSPLPAHLAHNGGAMVSLDVINEDYAAMENGEDPGSVINNNPVGTGFFKFDKWDPGSQIRLVKNE
EYWDEPAKLDSVVFKVVTEGLTRVAELQTGDSHISDPLSPNDVEQVEAMDGVSVTQQGSVSLSYVGFNMEKEPFDDPNVR
KAINMAIDKSQIIDGIYNGVGTPAIGPLAPDVFGYDENAPGLEYDPEQAKELLAEAGLEDGFSTTLWTNDSQERVDAATN
IQAQLKEFNIDVDVQVLEWGAYLEQTAQGEHDMFVLGWSTVTGDADYGLYALFHSNNVGDAGNRTFTKDEELDALLDEAR
QEPDPETRQQLYSEIQVKLTEVAPMLYIHHQDFLLGVSDKVQGLTQLPTQILQLKDVTLEQ
>Mature_541_residues
MKLSKKSLLLLVFGLLLSVVLVACASEPDEGGNTDSSGDSDADSEESTEGGEGGELVIATLSDAVSLDPAGSNDVPSSNV
QANIFESLTKQDANMEVQPSLATEWEAIDDTTWEFKLREGVKFHDGSDFNAEVVKANIERILDPDVGSPRLFLYEMIEEI
EVVDDYTIRFTTEFPFSPLPAHLAHNGGAMVSLDVINEDYAAMENGEDPGSVINNNPVGTGFFKFDKWDPGSQIRLVKNE
EYWDEPAKLDSVVFKVVTEGLTRVAELQTGDSHISDPLSPNDVEQVEAMDGVSVTQQGSVSLSYVGFNMEKEPFDDPNVR
KAINMAIDKSQIIDGIYNGVGTPAIGPLAPDVFGYDENAPGLEYDPEQAKELLAEAGLEDGFSTTLWTNDSQERVDAATN
IQAQLKEFNIDVDVQVLEWGAYLEQTAQGEHDMFVLGWSTVTGDADYGLYALFHSNNVGDAGNRTFTKDEELDALLDEAR
QEPDPETRQQLYSEIQVKLTEVAPMLYIHHQDFLLGVSDKVQGLTQLPTQILQLKDVTLEQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=496, Percent_Identity=32.258064516129, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789966, Length=469, Percent_Identity=30.7036247334755, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1787762, Length=479, Percent_Identity=26.9311064718163, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1787551, Length=459, Percent_Identity=24.6187363834423, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787495, Length=500, Percent_Identity=25.4, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081878, Length=541, Percent_Identity=24.5841035120148, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789397, Length=492, Percent_Identity=24.5934959349593, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789887, Length=459, Percent_Identity=25.9259259259259, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59294; Mature: 59294

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSKKSLLLLVFGLLLSVVLVACASEPDEGGNTDSSGDSDADSEESTEGGEGGELVIAT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
LSDAVSLDPAGSNDVPSSNVQANIFESLTKQDANMEVQPSLATEWEAIDDTTWEFKLREG
ECCCEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEECCC
VKFHDGSDFNAEVVKANIERILDPDVGSPRLFLYEMIEEIEVVDDYTIRFTTEFPFSPLP
CEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
AHLAHNGGAMVSLDVINEDYAAMENGEDPGSVINNNPVGTGFFKFDKWDPGSQIRLVKNE
HHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECC
EYWDEPAKLDSVVFKVVTEGLTRVAELQTGDSHISDPLSPNDVEQVEAMDGVSVTQQGSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCE
SLSYVGFNMEKEPFDDPNVRKAINMAIDKSQIIDGIYNGVGTPAIGPLAPDVFGYDENAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GLEYDPEQAKELLAEAGLEDGFSTTLWTNDSQERVDAATNIQAQLKEFNIDVDVQVLEWG
CCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
AYLEQTAQGEHDMFVLGWSTVTGDADYGLYALFHSNNVGDAGNRTFTKDEELDALLDEAR
HHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
QEPDPETRQQLYSEIQVKLTEVAPMLYIHHQDFLLGVSDKVQGLTQLPTQILQLKDVTLE
CCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCCC
Q
C
>Mature Secondary Structure
MKLSKKSLLLLVFGLLLSVVLVACASEPDEGGNTDSSGDSDADSEESTEGGEGGELVIAT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
LSDAVSLDPAGSNDVPSSNVQANIFESLTKQDANMEVQPSLATEWEAIDDTTWEFKLREG
ECCCEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEECCC
VKFHDGSDFNAEVVKANIERILDPDVGSPRLFLYEMIEEIEVVDDYTIRFTTEFPFSPLP
CEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
AHLAHNGGAMVSLDVINEDYAAMENGEDPGSVINNNPVGTGFFKFDKWDPGSQIRLVKNE
HHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECC
EYWDEPAKLDSVVFKVVTEGLTRVAELQTGDSHISDPLSPNDVEQVEAMDGVSVTQQGSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCE
SLSYVGFNMEKEPFDDPNVRKAINMAIDKSQIIDGIYNGVGTPAIGPLAPDVFGYDENAP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GLEYDPEQAKELLAEAGLEDGFSTTLWTNDSQERVDAATNIQAQLKEFNIDVDVQVLEWG
CCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHH
AYLEQTAQGEHDMFVLGWSTVTGDADYGLYALFHSNNVGDAGNRTFTKDEELDALLDEAR
HHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
QEPDPETRQQLYSEIQVKLTEVAPMLYIHHQDFLLGVSDKVQGLTQLPTQILQLKDVTLE
CCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA