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The map label for this gene is spo0F [H]

Identifier: 23100461

GI number: 23100461

Start: 3114909

End: 3115268

Strand: Reverse

Name: spo0F [H]

Synonym: OB3006

Alternate gene names: 23100461

Gene position: 3115268-3114909 (Counterclockwise)

Preceding gene: 23100462

Following gene: 23100460

Centisome position: 85.81

GC content: 31.67

Gene sequence:

>360_bases
ATGGGGAATTCAATTTTAGTAGTAGATGATCAACCAGGTATCAGATTGTTATTAAGTGAAGTGCTATCAAATAAAGGGTA
TACAGTAACCCTTGCAGAAACTGGAAAAGATGCAATCGATAAGCTAGGTGAAAAATTATATGATTTAGTGATTTTAGATT
ATAAGCTTCCTGTGTTGGATGGTAAGGAGGTAATTAAGCAAATGGACTCTTCCAAATACAATACAAAGATTGTTGTTATG
AGTGGAATGGCCGAAAGTATTCAAGAAGAAATAGCAGAATATAAAGTTGAAAAATTGATATCAAAACCGTTTAATTTAAA
TGATTTGTGCTCCGATGTAGAAGGTATATTAGAAGATTAA

Upstream 100 bases:

>100_bases
TAGGGGTTTGTAGAAAACTCTTTTTTTATTTAGCAGGATTATTTTTTGTAGTCATCGAATTTTATTACTACAAGAAATAA
AACACAGGGGGAGAAAAGTT

Downstream 100 bases:

>100_bases
TACAGATAGAATGAATCTAACTTGTTGCACCAGAGACTGGTAGTTGGTATTCTTAATAATGTAATAAAAATGATATTGTT
TTTTATTGCTATGTAAATAT

Product: stage 0 sporulation protein F

Products: NA

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 119; Mature: 118

Protein sequence:

>119_residues
MGNSILVVDDQPGIRLLLSEVLSNKGYTVTLAETGKDAIDKLGEKLYDLVILDYKLPVLDGKEVIKQMDSSKYNTKIVVM
SGMAESIQEEIAEYKVEKLISKPFNLNDLCSDVEGILED

Sequences:

>Translated_119_residues
MGNSILVVDDQPGIRLLLSEVLSNKGYTVTLAETGKDAIDKLGEKLYDLVILDYKLPVLDGKEVIKQMDSSKYNTKIVVM
SGMAESIQEEIAEYKVEKLISKPFNLNDLCSDVEGILED
>Mature_118_residues
GNSILVVDDQPGIRLLLSEVLSNKGYTVTLAETGKDAIDKLGEKLYDLVILDYKLPVLDGKEVIKQMDSSKYNTKIVVMS
GMAESIQEEIAEYKVEKLISKPFNLNDLCSDVEGILED

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=30.7017543859649, Blast_Score=62, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13197; Mature: 13066

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSILVVDDQPGIRLLLSEVLSNKGYTVTLAETGKDAIDKLGEKLYDLVILDYKLPVLD
CCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC
GKEVIKQMDSSKYNTKIVVMSGMAESIQEEIAEYKVEKLISKPFNLNDLCSDVEGILED
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNSILVVDDQPGIRLLLSEVLSNKGYTVTLAETGKDAIDKLGEKLYDLVILDYKLPVLD
CCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC
GKEVIKQMDSSKYNTKIVVMSGMAESIQEEIAEYKVEKLISKPFNLNDLCSDVEGILED
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3003689; 2997779; 2457578; 9353933; 9384377; 8528078; 9254596; 8805550; 9335530; 10997904 [H]