Click here to switch to the map view.

The map label for this gene is glyA

Identifier: 23100440

GI number: 23100440

Start: 3095718

End: 3096953

Strand: Reverse

Name: glyA

Synonym: OB2985

Alternate gene names: 23100440

Gene position: 3096953-3095718 (Counterclockwise)

Preceding gene: 23100441

Following gene: 23100439

Centisome position: 85.3

GC content: 37.3

Gene sequence:

>1236_bases
ATGGAATTTGTGAAACAAGCAGATACCGAAGTGTTTGAAGCAATGCAAGCCGAAAAAAATCGCCAGCAAGATAAGATTGA
GTTAATTGCATCCGAAAATTTTGTAACAAAAGCAGTAATGGATGCGATGGGATCTATTCTTACCAATAAATATGCAGAAG
GTTATCCAGGCAAGCGTTATTATGGTGGTTGTGAGCATGTTGATGTAGTGGAAAATCTTGCTCGTGATCGTGCGAAAGAA
TTATTTGGTGCTGATCACGCAAATGTACAACCTCATTCAGGAGCACAAGCAAATATGGCAGTTTATTCTGCGGTACTTGA
GCCTGGTGATACGGTACTAGGTATGAATCTAAATCATGGTGGACATTTAACTCATGGTAGCCCAGTGAACTTTAGCGGTC
AATTGTATAATTTTGTTGACTATGGTGTAGACAAAGAGACAGAACAACTTGATTATGATGCTGTATTGGAAAAAGCAAAG
GAAGTAAAACCTAAATTAATCGTGGCTGGAGCAAGTGCATACTCCCGTTCTATTAATTTTGCTAAATTCCGTGAAATTGC
TGATGCAGTAGATGCATATCTAATGGTAGATATGGCACATATTGCTGGTTTAGTTGCTACTGGTGAACATGAAAATCCAG
TTCCTCATGCAGATTTTGTTACTACTACAACCCATAAAACATTGCGCGGACCTCGTGGTGGTATGATTCTTTGTAAAGAA
GAATTTGCCAAAAAAGTAGATAAAGCGATTTTCCCTGGAATCCAAGGTGGACCGTTAATGCATGTTATTGCTGCAAAAGC
TGTATCCTTTAAAGAAGCATTGTCAGATGACTTTAAAGCATATTCAAAGCAAATTGTTGCAAATGCAAAATTACTAGGGG
AAGCTCTCAATAAAGAAGGAATTCGTATTGTATCTGGAGGTACAGATAACCATCTACTATTATTAGATGTTACTCCATTA
CAATTAACCGGAAAAGTAGCTGAAAAAGCACTAGATGATATCGGTATTACAACGAATAAAAACACGATTCCTTTTGATCA
AGAAAGTCCTTTTGTAACAAGTGGTATCCGTATTGGTACTGCTGCAGTAACTACACGTGGATTTGGTGAAGAAGAAATGA
AAGAAATTGCTTCTATTATTTCTTTAACGTTAAAACACCATGAAGATGAAGCGAAGTTAAAAGAAGCAGCACAACGTGTA
CAAGCACTTACAGAAAAATTCACGCTATATGCTTAA

Upstream 100 bases:

>100_bases
AGTAGGGGGAAGTAGAGCTTACTATGGCAAGTAGTCATTATAACTACCATCTTCATCAGTAAATATTTAAAATCTTATTA
CATAGGGGGCTATACCAATG

Downstream 100 bases:

>100_bases
ATAATAGAAACGAACAATAATTGGATGATACTACATTATCCACTATTGTTCGTTTTTTTACTTAATGAGAAGATTGAAAA
GTGCTCTTCTTTTCAAGTTA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MEFVKQADTEVFEAMQAEKNRQQDKIELIASENFVTKAVMDAMGSILTNKYAEGYPGKRYYGGCEHVDVVENLARDRAKE
LFGADHANVQPHSGAQANMAVYSAVLEPGDTVLGMNLNHGGHLTHGSPVNFSGQLYNFVDYGVDKETEQLDYDAVLEKAK
EVKPKLIVAGASAYSRSINFAKFREIADAVDAYLMVDMAHIAGLVATGEHENPVPHADFVTTTTHKTLRGPRGGMILCKE
EFAKKVDKAIFPGIQGGPLMHVIAAKAVSFKEALSDDFKAYSKQIVANAKLLGEALNKEGIRIVSGGTDNHLLLLDVTPL
QLTGKVAEKALDDIGITTNKNTIPFDQESPFVTSGIRIGTAAVTTRGFGEEEMKEIASIISLTLKHHEDEAKLKEAAQRV
QALTEKFTLYA

Sequences:

>Translated_411_residues
MEFVKQADTEVFEAMQAEKNRQQDKIELIASENFVTKAVMDAMGSILTNKYAEGYPGKRYYGGCEHVDVVENLARDRAKE
LFGADHANVQPHSGAQANMAVYSAVLEPGDTVLGMNLNHGGHLTHGSPVNFSGQLYNFVDYGVDKETEQLDYDAVLEKAK
EVKPKLIVAGASAYSRSINFAKFREIADAVDAYLMVDMAHIAGLVATGEHENPVPHADFVTTTTHKTLRGPRGGMILCKE
EFAKKVDKAIFPGIQGGPLMHVIAAKAVSFKEALSDDFKAYSKQIVANAKLLGEALNKEGIRIVSGGTDNHLLLLDVTPL
QLTGKVAEKALDDIGITTNKNTIPFDQESPFVTSGIRIGTAAVTTRGFGEEEMKEIASIISLTLKHHEDEAKLKEAAQRV
QALTEKFTLYA
>Mature_411_residues
MEFVKQADTEVFEAMQAEKNRQQDKIELIASENFVTKAVMDAMGSILTNKYAEGYPGKRYYGGCEHVDVVENLARDRAKE
LFGADHANVQPHSGAQANMAVYSAVLEPGDTVLGMNLNHGGHLTHGSPVNFSGQLYNFVDYGVDKETEQLDYDAVLEKAK
EVKPKLIVAGASAYSRSINFAKFREIADAVDAYLMVDMAHIAGLVATGEHENPVPHADFVTTTTHKTLRGPRGGMILCKE
EFAKKVDKAIFPGIQGGPLMHVIAAKAVSFKEALSDDFKAYSKQIVANAKLLGEALNKEGIRIVSGGTDNHLLLLDVTPL
QLTGKVAEKALDDIGITTNKNTIPFDQESPFVTSGIRIGTAAVTTRGFGEEEMKEIASIISLTLKHHEDEAKLKEAAQRV
QALTEKFTLYA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=41.7218543046358, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=41.7218543046358, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=41.7218543046358, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=41.7218543046358, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI261862346, Length=448, Percent_Identity=41.7410714285714, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI22547186, Length=448, Percent_Identity=43.5267857142857, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI22547189, Length=433, Percent_Identity=43.1870669745958, Blast_Score=312, Evalue=3e-85,
Organism=Escherichia coli, GI1788902, Length=407, Percent_Identity=61.4250614250614, Blast_Score=493, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25144732, Length=403, Percent_Identity=46.1538461538462, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25144729, Length=403, Percent_Identity=46.1538461538462, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319739, Length=410, Percent_Identity=48.0487804878049, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323087, Length=418, Percent_Identity=42.3444976076555, Blast_Score=319, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24640005, Length=411, Percent_Identity=45.985401459854, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI221329721, Length=411, Percent_Identity=45.985401459854, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_OCEIH (Q8EM73)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693907.1
- ProteinModelPortal:   Q8EM73
- SMR:   Q8EM73
- GeneID:   1016340
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2985
- NMPDR:   fig|221109.1.peg.2988
- HOGENOM:   HBG301263
- OMA:   NADESRE
- BioCyc:   OIHE221109:OB2985-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44741; Mature: 44741

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFVKQADTEVFEAMQAEKNRQQDKIELIASENFVTKAVMDAMGSILTNKYAEGYPGKRY
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
YGGCEHVDVVENLARDRAKELFGADHANVQPHSGAQANMAVYSAVLEPGDTVLGMNLNHG
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
GHLTHGSPVNFSGQLYNFVDYGVDKETEQLDYDAVLEKAKEVKPKLIVAGASAYSRSINF
CCCCCCCCCCCCCHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCEEEEEECHHHHHCCCH
AKFREIADAVDAYLMVDMAHIAGLVATGEHENPVPHADFVTTTTHKTLRGPRGGMILCKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHCCCCCCEEEEHH
EFAKKVDKAIFPGIQGGPLMHVIAAKAVSFKEALSDDFKAYSKQIVANAKLLGEALNKEG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IRIVSGGTDNHLLLLDVTPLQLTGKVAEKALDDIGITTNKNTIPFDQESPFVTSGIRIGT
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEECH
AAVTTRGFGEEEMKEIASIISLTLKHHEDEAKLKEAAQRVQALTEKFTLYA
HHHEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFVKQADTEVFEAMQAEKNRQQDKIELIASENFVTKAVMDAMGSILTNKYAEGYPGKRY
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
YGGCEHVDVVENLARDRAKELFGADHANVQPHSGAQANMAVYSAVLEPGDTVLGMNLNHG
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
GHLTHGSPVNFSGQLYNFVDYGVDKETEQLDYDAVLEKAKEVKPKLIVAGASAYSRSINF
CCCCCCCCCCCCCHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCCEEEEEECHHHHHCCCH
AKFREIADAVDAYLMVDMAHIAGLVATGEHENPVPHADFVTTTTHKTLRGPRGGMILCKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHCCCCCCEEEEHH
EFAKKVDKAIFPGIQGGPLMHVIAAKAVSFKEALSDDFKAYSKQIVANAKLLGEALNKEG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IRIVSGGTDNHLLLLDVTPLQLTGKVAEKALDDIGITTNKNTIPFDQESPFVTSGIRIGT
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEECH
AAVTTRGFGEEEMKEIASIISLTLKHHEDEAKLKEAAQRVQALTEKFTLYA
HHHEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376