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The map label for this gene is ykfC [H]

Identifier: 23100419

GI number: 23100419

Start: 3072823

End: 3073752

Strand: Reverse

Name: ykfC [H]

Synonym: OB2964

Alternate gene names: 23100419

Gene position: 3073752-3072823 (Counterclockwise)

Preceding gene: 23100420

Following gene: 23100418

Centisome position: 84.66

GC content: 38.39

Gene sequence:

>930_bases
ATGAAACAAACGTTTGATCAATTATCCGAAGATTTTTGGGTGACCGATGTACAAGTGGCAACAGTATGGACGAATCCACA
GTCGGCCAGGGATATAGATAAAGATGGGCGAACGAATCCGACAAATGTAAATCAATGGTATGAGCAACTTTCCTATGATG
AACGATTAGCGCTTTGTAATGAAAATAGAGTACAAACACAAGCATTATATGGCGAACCAGTTATAGTAATTAATGAATTA
GATGATTGGGCGGAAGTTATCTTGCCATTTCAAGCTTCGAAGAAAGATAAAAGAGGATATCCTGGCTGGGTGCCAAAGCA
ACAATTAACCATGACAACGAAAACACATTGGGTAAGAGATTCTATGGCGATGGTTATAAAAAACAAGAGTTGGTTAGAAA
CCCAAGAAGGTGAAAAAACACTTGAAATTAGCTTTGCAACCATTCTACCTGTAGTAACTGAACAAGAGATGATACAAGTG
CAAACTCCACATGGTATACAATATATAGCTAAAGAAGATGTACTTATTTTTCCTACAAAGGAAGGAGTACCTACTCGAAC
TGGAGAGGACATCGTTCGAACCGGAATGCTATTTTTACAATTAGATTATTTCTGGGGAGGAATGAGCTCTTTTGGCTACG
ATTGTTCCGGTCTGGCTTATGCGGCTCATAAGGCGAATGGATATTGGATCGCCAGAGATGCAGGTGATCAAGCAGTCGGT
GGAAAAGAAATACCGTTTGATGAGGTACAAGCTGGAGATTTACTGTTTTTTGCCTATGAAGAAGGAAAAGGAAATATTCA
TCATGTAGGAATATATAAAGGAAATGGTGAAATGTTACATTCACCACAAACAGGTAAAGGAATCGAAATCACTCCATTAG
AAGGAACCAAATATGAAAAAGAATTATGTGCAGCACGAAGATATTGGTAA

Upstream 100 bases:

>100_bases
TATCTTTTAGTGAGGATTATGGATTAGGAATCTGCGGAATTCGCTCTGAGTTTTTGAGACAAAACACAATGAAATAATTT
CAGAATGGGGGAGCTGAAAT

Downstream 100 bases:

>100_bases
ATAGGAGGGTTGAGAATGCAAGATCATATTCTAGAAGTTCGTAACTTAAAAAAGTATTTTCAAGTTGGCAAGGGAAGTTT
ATTAAAAGCAGTTGATGATA

Product: polysugar degrading enzyme

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKQTFDQLSEDFWVTDVQVATVWTNPQSARDIDKDGRTNPTNVNQWYEQLSYDERLALCNENRVQTQALYGEPVIVINEL
DDWAEVILPFQASKKDKRGYPGWVPKQQLTMTTKTHWVRDSMAMVIKNKSWLETQEGEKTLEISFATILPVVTEQEMIQV
QTPHGIQYIAKEDVLIFPTKEGVPTRTGEDIVRTGMLFLQLDYFWGGMSSFGYDCSGLAYAAHKANGYWIARDAGDQAVG
GKEIPFDEVQAGDLLFFAYEEGKGNIHHVGIYKGNGEMLHSPQTGKGIEITPLEGTKYEKELCAARRYW

Sequences:

>Translated_309_residues
MKQTFDQLSEDFWVTDVQVATVWTNPQSARDIDKDGRTNPTNVNQWYEQLSYDERLALCNENRVQTQALYGEPVIVINEL
DDWAEVILPFQASKKDKRGYPGWVPKQQLTMTTKTHWVRDSMAMVIKNKSWLETQEGEKTLEISFATILPVVTEQEMIQV
QTPHGIQYIAKEDVLIFPTKEGVPTRTGEDIVRTGMLFLQLDYFWGGMSSFGYDCSGLAYAAHKANGYWIARDAGDQAVG
GKEIPFDEVQAGDLLFFAYEEGKGNIHHVGIYKGNGEMLHSPQTGKGIEITPLEGTKYEKELCAARRYW
>Mature_309_residues
MKQTFDQLSEDFWVTDVQVATVWTNPQSARDIDKDGRTNPTNVNQWYEQLSYDERLALCNENRVQTQALYGEPVIVINEL
DDWAEVILPFQASKKDKRGYPGWVPKQQLTMTTKTHWVRDSMAMVIKNKSWLETQEGEKTLEISFATILPVVTEQEMIQV
QTPHGIQYIAKEDVLIFPTKEGVPTRTGEDIVRTGMLFLQLDYFWGGMSSFGYDCSGLAYAAHKANGYWIARDAGDQAVG
GKEIPFDEVQAGDLLFFAYEEGKGNIHHVGIYKGNGEMLHSPQTGKGIEITPLEGTKYEKELCAARRYW

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=116, Percent_Identity=39.6551724137931, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788001, Length=106, Percent_Identity=34.9056603773585, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 35134; Mature: 35134

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTFDQLSEDFWVTDVQVATVWTNPQSARDIDKDGRTNPTNVNQWYEQLSYDERLALCN
CCCHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECC
ENRVQTQALYGEPVIVINELDDWAEVILPFQASKKDKRGYPGWVPKQQLTMTTKTHWVRD
CCCEEEEEECCCCEEEEECCCCHHHEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
SMAMVIKNKSWLETQEGEKTLEISFATILPVVTEQEMIQVQTPHGIQYIAKEDVLIFPTK
CEEEEEECCCCCCCCCCCEEEEEEHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECC
EGVPTRTGEDIVRTGMLFLQLDYFWGGMSSFGYDCSGLAYAAHKANGYWIARDAGDQAVG
CCCCCCCCHHHHHHCEEEEEEEHHHCCCHHCCCCCCCEEEEEECCCCEEEEECCCCCCCC
GKEIPFDEVQAGDLLFFAYEEGKGNIHHVGIYKGNGEMLHSPQTGKGIEITPLEGTKYEK
CCCCCHHHCCCCCEEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCEEEEECCCCCHHH
ELCAARRYW
HHHHHHCCC
>Mature Secondary Structure
MKQTFDQLSEDFWVTDVQVATVWTNPQSARDIDKDGRTNPTNVNQWYEQLSYDERLALCN
CCCHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECC
ENRVQTQALYGEPVIVINELDDWAEVILPFQASKKDKRGYPGWVPKQQLTMTTKTHWVRD
CCCEEEEEECCCCEEEEECCCCHHHEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
SMAMVIKNKSWLETQEGEKTLEISFATILPVVTEQEMIQVQTPHGIQYIAKEDVLIFPTK
CEEEEEECCCCCCCCCCCEEEEEEHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECC
EGVPTRTGEDIVRTGMLFLQLDYFWGGMSSFGYDCSGLAYAAHKANGYWIARDAGDQAVG
CCCCCCCCHHHHHHCEEEEEEEHHHCCCHHCCCCCCCEEEEEECCCCEEEEECCCCCCCC
GKEIPFDEVQAGDLLFFAYEEGKGNIHHVGIYKGNGEMLHSPQTGKGIEITPLEGTKYEK
CCCCCHHHCCCCCEEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCEEEEECCCCCHHH
ELCAARRYW
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]