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The map label for this gene is opuAB [H]

Identifier: 23100398

GI number: 23100398

Start: 3049972

End: 3050829

Strand: Direct

Name: opuAB [H]

Synonym: OB2943

Alternate gene names: 23100398

Gene position: 3049972-3050829 (Clockwise)

Preceding gene: 23100397

Following gene: 23100399

Centisome position: 84.01

GC content: 36.6

Gene sequence:

>858_bases
ATGGGAATATTTCCAGATATAGAGTTTGAAGCTGGTAAGTATGTAGATCAAGCCGTTAGTTTTATTGATTCTACATTTGA
AGCTTTTTTTGATTTTATTTTCTTTATTGCATCCCGTTCTATTAATCTAATTACAGATGCATTAATGTTTTTGCCTTGGT
GGTTATTCATTATTATTATTATTGCATTAGGATGGTACTTTAAGTCGGCCTTTTCCGGTATTCTTTATGGTGCGTTTATC
TTTATCATCGGAACATTTGGATTATGGGAAGATATGATGATTACCATCTCCATTATTCTCACAGCAGTAATTATATGTTT
ACTTATCGGCATTCCAATTGGTGTAGCAATGTCATTTAGTAAACTATTCTCGACTATTGCCCGCCCAGTACTTGATGCAA
TGCAGACAATGCCTAGTTTTGTCTACCTCATTCCAGCGATTTTCTTCTTTGGCTTAGGTAATGTTTCTGCTATTTTTGCA
ACGCTGATTTATGCTTTACCTCCTGTTATTCGTTTAACGGAGCTTGCTATTCGTGGAGTAGATAAGGAGATTATCGAATC
TGCACATTCCTTTGGTTCATCAAAATGGCAAACATTAAAAAAAGTACAATTACCACAGGCAACACCTACAATTATGGCTG
GTGTGAACCAAACAACTATGATGGCATTATCAATGGTCGTAATCGCTTCGATGGTTGGTGCATCTGGATTAGGTGAACAA
GTACTTGTATCAATTAATCGCATAGATATCGCACTAGGATTCGAAGCTGGAATCAGTATCGTGTTCTTAGCGGTTATCAT
TGACCGTATTACTAACGGTGTTGCACAAAAATTCCAAAGACAGGGTGATAGAGCATGA

Upstream 100 bases:

>100_bases
AATGGAGCAGCTTGCCACTTGGTTGGCTGTTCTTTCTATTTAAAATATCATTTATTAATGAAAGAGAGGTGTAGAACTGC
TAGACGCAGTATCGACGCTT

Downstream 100 bases:

>100_bases
CAACCCATATTAAAGTAGAAAATGTATCGAAAATATTTGGTCCAAAACCTAAATCCGTGATTCCAATGGTGAAAGAAGGA
ATGTCAAAGGCAGACATACT

Product: ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MGIFPDIEFEAGKYVDQAVSFIDSTFEAFFDFIFFIASRSINLITDALMFLPWWLFIIIIIALGWYFKSAFSGILYGAFI
FIIGTFGLWEDMMITISIILTAVIICLLIGIPIGVAMSFSKLFSTIARPVLDAMQTMPSFVYLIPAIFFFGLGNVSAIFA
TLIYALPPVIRLTELAIRGVDKEIIESAHSFGSSKWQTLKKVQLPQATPTIMAGVNQTTMMALSMVVIASMVGASGLGEQ
VLVSINRIDIALGFEAGISIVFLAVIIDRITNGVAQKFQRQGDRA

Sequences:

>Translated_285_residues
MGIFPDIEFEAGKYVDQAVSFIDSTFEAFFDFIFFIASRSINLITDALMFLPWWLFIIIIIALGWYFKSAFSGILYGAFI
FIIGTFGLWEDMMITISIILTAVIICLLIGIPIGVAMSFSKLFSTIARPVLDAMQTMPSFVYLIPAIFFFGLGNVSAIFA
TLIYALPPVIRLTELAIRGVDKEIIESAHSFGSSKWQTLKKVQLPQATPTIMAGVNQTTMMALSMVVIASMVGASGLGEQ
VLVSINRIDIALGFEAGISIVFLAVIIDRITNGVAQKFQRQGDRA
>Mature_284_residues
GIFPDIEFEAGKYVDQAVSFIDSTFEAFFDFIFFIASRSINLITDALMFLPWWLFIIIIIALGWYFKSAFSGILYGAFIF
IIGTFGLWEDMMITISIILTAVIICLLIGIPIGVAMSFSKLFSTIARPVLDAMQTMPSFVYLIPAIFFFGLGNVSAIFAT
LIYALPPVIRLTELAIRGVDKEIIESAHSFGSSKWQTLKKVQLPQATPTIMAGVNQTTMMALSMVVIASMVGASGLGEQV
LVSINRIDIALGFEAGISIVFLAVIIDRITNGVAQKFQRQGDRA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=272, Percent_Identity=41.9117647058824, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1788449, Length=174, Percent_Identity=27.5862068965517, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788451, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31305; Mature: 31173

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GIFPDIEFEAGKYVDQAVSFIDSTFEAFFDFIFFIASRSINLITDALMFLPWWLFIIII
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IALGWYFKSAFSGILYGAFIFIIGTFGLWEDMMITISIILTAVIICLLIGIPIGVAMSFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFSTIARPVLDAMQTMPSFVYLIPAIFFFGLGNVSAIFATLIYALPPVIRLTELAIRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHCC
DKEIIESAHSFGSSKWQTLKKVQLPQATPTIMAGVNQTTMMALSMVVIASMVGASGLGEQ
CHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHH
VLVSINRIDIALGFEAGISIVFLAVIIDRITNGVAQKFQRQGDRA
HHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]