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The map label for this gene is epsM [H]

Identifier: 23100346

GI number: 23100346

Start: 2979246

End: 2979872

Strand: Reverse

Name: epsM [H]

Synonym: OB2891

Alternate gene names: 23100346

Gene position: 2979872-2979246 (Counterclockwise)

Preceding gene: 23100347

Following gene: 23100345

Centisome position: 82.08

GC content: 31.58

Gene sequence:

>627_bases
ATGAGTAATAAAAAATTGTTGATTATTGGTGCTAGTGGTCATGGTAAAGTAATAGCGGATATCGCTAAAAAAATGAATCA
ATGGGATGAAATTGCATTTTTGGATGATAATCAATCAATTAACAATTGTATAGGTCTAAAAGTAATTGGAATTTCCAGTG
ATTTTGTAAATTATATTAATGATTATCAAATCGTTGTTGGGATAGGTAATAATAATGTTAGGGAGAATATTTATAATCAA
TTAGAGTTTCTTGACGCAACAGTAGCAACCTTGATTCATCCTGATGCTGTAATAGGTTTCAATGTAAACATTGGAAAAGG
ATCAGTAGTGATGGCAGGTACAGTAATTAATAGTTGTACAAAGATTGGTAAGGGATGCATTATCAATACCGGTGCAACTA
TAGATCACGACAATTGTATTGGTGACTTTGTTCATATTTCTCCGGGAGTACATTTAGCAGGAACTGTAATAATTGGAAAA
AGTTCTTGGCTTGGTATAGGATCTATTGTTAATAACAATGTAGACATTACAAAAGAATGTATTATCGGAGCCAATTCATT
TGTAAAAGAAAATATAAAAGAAAGCGGTACATATGTAGGTGTACCTACAAGGAGAATTTCAACATGA

Upstream 100 bases:

>100_bases
TTTTTAACTTTTAAAAAGGTCTTTATACGTGAAGGTATAAATTCTGAAACTACTGCTACGATTGAACCATTTCAAGGGAA
TGAAAAGGAAAAGTTTTATA

Downstream 100 bases:

>100_bases
GAGTATTGATTCTTGCTAACTTCGGAATGGGTTTATACAATTTTAGGAGAGAGTTAATTGAGAGATTATTAGAGGAAGAA
TATGAGGTATACATATCCTC

Product: acetyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSNKKLLIIGASGHGKVIADIAKKMNQWDEIAFLDDNQSINNCIGLKVIGISSDFVNYINDYQIVVGIGNNNVRENIYNQ
LEFLDATVATLIHPDAVIGFNVNIGKGSVVMAGTVINSCTKIGKGCIINTGATIDHDNCIGDFVHISPGVHLAGTVIIGK
SSWLGIGSIVNNNVDITKECIIGANSFVKENIKESGTYVGVPTRRIST

Sequences:

>Translated_208_residues
MSNKKLLIIGASGHGKVIADIAKKMNQWDEIAFLDDNQSINNCIGLKVIGISSDFVNYINDYQIVVGIGNNNVRENIYNQ
LEFLDATVATLIHPDAVIGFNVNIGKGSVVMAGTVINSCTKIGKGCIINTGATIDHDNCIGDFVHISPGVHLAGTVIIGK
SSWLGIGSIVNNNVDITKECIIGANSFVKENIKESGTYVGVPTRRIST
>Mature_207_residues
SNKKLLIIGASGHGKVIADIAKKMNQWDEIAFLDDNQSINNCIGLKVIGISSDFVNYINDYQIVVGIGNNNVRENIYNQL
EFLDATVATLIHPDAVIGFNVNIGKGSVVMAGTVINSCTKIGKGCIINTGATIDHDNCIGDFVHISPGVHLAGTVIIGKS
SWLGIGSIVNNNVDITKECIIGANSFVKENIKESGTYVGVPTRRIST

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 22174; Mature: 22043

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKLLIIGASGHGKVIADIAKKMNQWDEIAFLDDNQSINNCIGLKVIGISSDFVNYIN
CCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEECHHHHHHCC
DYQIVVGIGNNNVRENIYNQLEFLDATVATLIHPDAVIGFNVNIGKGSVVMAGTVINSCT
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHHH
KIGKGCIINTGATIDHDNCIGDFVHISPGVHLAGTVIIGKSSWLGIGSIVNNNVDITKEC
HHCCCEEEECCCEECCCCCCCCEEEECCCCEEEEEEEEECCCCEEEHHHHCCCCCEEHHE
IIGANSFVKENIKESGTYVGVPTRRIST
EECCHHHHHHHHHHCCCEEECCCCCCCC
>Mature Secondary Structure 
SNKKLLIIGASGHGKVIADIAKKMNQWDEIAFLDDNQSINNCIGLKVIGISSDFVNYIN
CCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEECHHHHHHCC
DYQIVVGIGNNNVRENIYNQLEFLDATVATLIHPDAVIGFNVNIGKGSVVMAGTVINSCT
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHHH
KIGKGCIINTGATIDHDNCIGDFVHISPGVHLAGTVIIGKSSWLGIGSIVNNNVDITKEC
HHCCCEEEECCCEECCCCCCCCEEEECCCCEEEEEEEEECCCCEEEHHHHCCCCCEEHHE
IIGANSFVKENIKESGTYVGVPTRRIST
EECCHHHHHHHHHHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]