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The map label for this gene is bsdA [H]

Identifier: 23100328

GI number: 23100328

Start: 2958496

End: 2959386

Strand: Direct

Name: bsdA [H]

Synonym: OB2873

Alternate gene names: 23100328

Gene position: 2958496-2959386 (Clockwise)

Preceding gene: 23100313

Following gene: 23100357

Centisome position: 81.49

GC content: 36.48

Gene sequence:

>891_bases
ATGGATATTAGACAACTGCGTTACTTTTATACGATTGCAACAGAAAAACAAATTACAAGAGCAGCAAAAAAACTTCATAT
GGCCCAGCCTCCATTAAGCCAAAGTTTAAAAGCATTAGAAGAAGAAATTGGTGTCCCGCTATTTGAAAGAAATGGCAAGA
AAATGGAACTAACGGATGCAGGTGAAGTTTTATACAATAAAGCAGATTATTTTTTTAAGTTTTTTGATGAAGCGCTTACA
GAAGTTAAAGAAACGGGTGAAGGGCTAAAAGGACAGTTGACCGTAGGCTGTGTAAAAACACTTTTTTCACACGTACCACA
ACATATTAAATCTTTTCGCGAGAAATATCCAAACGTTTCATTTGAATTAAGAGAAGGGGACTCTTACCTTCTTGCCGAGC
AACTGAAAAATCGAAACATTGATTTGGCGATCGTTCGTCTTCCTCTAGATATGGAACCTTATTGTAGCCTCTTTCTACCT
GATGAAAACTATGTGGCTATTATGCCAGAACAATGGGCAAATTCCTATGAAACTGACACCATTACGATGAACGAACTTGC
CGATTTACCTTTAATATTATTAAGAAGAATTAGCGGCGTTGGTCAATACGAGTTGATTATGGACAGATTTGAAAAAAGAG
GACTTACTCCCAATATCGTGACAGTTTGTCCGGATGTGGATATGATTCTTGAATTGGTTAATGTGGAAGTTGGAGCTTCC
ATTGTTCCAGCATCCACGCTTAAAAAACATTCTATTTCAGGTATAAAAAGTTTTGCTATTAAAGACGAAACGATTATTTC
TAAGTCCGCGATTATTTGGCTAAAGGACCGTTATGTTTCCAAAAGCAAACAACGTTTTATCGATTTTTTTGAGGAGTCCA
TTGACAAATGA

Upstream 100 bases:

>100_bases
AGGCTAGTTAAATTTTTTGACTTTTTTGTTATTTGGTACTATTATTTATACATATTTGGATTATGTATTGCTTAGACAAG
AGGTATCGGGGTGAAGAAAG

Downstream 100 bases:

>100_bases
AAAACGGCTAATTCCTTCAAATTTAATGTAAAAAGAAGGAATTAGCCGTTTTTTAATATACTGTATAAACAACGAATCAC
ACTAGCCAACTATGACCACA

Product: transcriptional regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDIRQLRYFYTIATEKQITRAAKKLHMAQPPLSQSLKALEEEIGVPLFERNGKKMELTDAGEVLYNKADYFFKFFDEALT
EVKETGEGLKGQLTVGCVKTLFSHVPQHIKSFREKYPNVSFELREGDSYLLAEQLKNRNIDLAIVRLPLDMEPYCSLFLP
DENYVAIMPEQWANSYETDTITMNELADLPLILLRRISGVGQYELIMDRFEKRGLTPNIVTVCPDVDMILELVNVEVGAS
IVPASTLKKHSISGIKSFAIKDETIISKSAIIWLKDRYVSKSKQRFIDFFEESIDK

Sequences:

>Translated_296_residues
MDIRQLRYFYTIATEKQITRAAKKLHMAQPPLSQSLKALEEEIGVPLFERNGKKMELTDAGEVLYNKADYFFKFFDEALT
EVKETGEGLKGQLTVGCVKTLFSHVPQHIKSFREKYPNVSFELREGDSYLLAEQLKNRNIDLAIVRLPLDMEPYCSLFLP
DENYVAIMPEQWANSYETDTITMNELADLPLILLRRISGVGQYELIMDRFEKRGLTPNIVTVCPDVDMILELVNVEVGAS
IVPASTLKKHSISGIKSFAIKDETIISKSAIIWLKDRYVSKSKQRFIDFFEESIDK
>Mature_296_residues
MDIRQLRYFYTIATEKQITRAAKKLHMAQPPLSQSLKALEEEIGVPLFERNGKKMELTDAGEVLYNKADYFFKFFDEALT
EVKETGEGLKGQLTVGCVKTLFSHVPQHIKSFREKYPNVSFELREGDSYLLAEQLKNRNIDLAIVRLPLDMEPYCSLFLP
DENYVAIMPEQWANSYETDTITMNELADLPLILLRRISGVGQYELIMDRFEKRGLTPNIVTVCPDVDMILELVNVEVGAS
IVPASTLKKHSISGIKSFAIKDETIISKSAIIWLKDRYVSKSKQRFIDFFEESIDK

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=27.8350515463918, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI157672245, Length=294, Percent_Identity=24.8299319727891, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=27.7551020408163, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=26.2096774193548, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788748, Length=250, Percent_Identity=27.6, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=28.2868525896414, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=32.6530612244898, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790399, Length=197, Percent_Identity=29.4416243654822, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790208, Length=149, Percent_Identity=32.2147651006711, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI145693105, Length=300, Percent_Identity=25.3333333333333, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788296, Length=260, Percent_Identity=27.6923076923077, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787806, Length=308, Percent_Identity=21.1038961038961, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789204, Length=145, Percent_Identity=28.9655172413793, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788481, Length=305, Percent_Identity=22.9508196721311, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=141, Percent_Identity=28.3687943262411, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=145, Percent_Identity=29.6551724137931, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082184, Length=147, Percent_Identity=29.2517006802721, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33934; Mature: 33934

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRQLRYFYTIATEKQITRAAKKLHMAQPPLSQSLKALEEEIGVPLFERNGKKMELTDA
CCHHHEEEEHEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCEEEECCH
GEVLYNKADYFFKFFDEALTEVKETGEGLKGQLTVGCVKTLFSHVPQHIKSFREKYPNVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCE
FELREGDSYLLAEQLKNRNIDLAIVRLPLDMEPYCSLFLPDENYVAIMPEQWANSYETDT
EEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCHHHCCCCCCCE
ITMNELADLPLILLRRISGVGQYELIMDRFEKRGLTPNIVTVCPDVDMILELVNVEVGAS
EEHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCC
IVPASTLKKHSISGIKSFAIKDETIISKSAIIWLKDRYVSKSKQRFIDFFEESIDK
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIRQLRYFYTIATEKQITRAAKKLHMAQPPLSQSLKALEEEIGVPLFERNGKKMELTDA
CCHHHEEEEHEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCEEEECCH
GEVLYNKADYFFKFFDEALTEVKETGEGLKGQLTVGCVKTLFSHVPQHIKSFREKYPNVS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCE
FELREGDSYLLAEQLKNRNIDLAIVRLPLDMEPYCSLFLPDENYVAIMPEQWANSYETDT
EEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCHHHCCCCCCCE
ITMNELADLPLILLRRISGVGQYELIMDRFEKRGLTPNIVTVCPDVDMILELVNVEVGAS
EEHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCC
IVPASTLKKHSISGIKSFAIKDETIISKSAIIWLKDRYVSKSKQRFIDFFEESIDK
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]