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The map label for this gene is dapA [C]

Identifier: 23100296

GI number: 23100296

Start: 2924846

End: 2925772

Strand: Reverse

Name: dapA [C]

Synonym: OB2841

Alternate gene names: 23100296

Gene position: 2925772-2924846 (Counterclockwise)

Preceding gene: 23100297

Following gene: 23100295

Centisome position: 80.59

GC content: 36.46

Gene sequence:

>927_bases
ATGAATAGAAAAGCGCCAACTGGAATACTAGGATTCCCTGTGACGCCGTTTGATAACCAAGGGAATATAGATGAATTAGC
ATTATCTCAGAATATCAAGTACTTACTTGACTCAGGATTAGAGGCAATTTTTGTAGCATGTGGTGCTGGAGAATACCATT
CACTGGAAAATGGGGAATATGAGTCTATTATCGAAATTGCTACATCGACAGTGAAAGGACAGGTGCCTGTATACACTGGT
GTCGGAGGTAATCTCAGTGATGCAGTACACAAAGCTAAACTCTCTGAAAAACATGGTGTAGATGGATATTTAATTATGCC
AGCTTACCTTATTAATGGAGAGCAAGAAGGTATTTATCAATATGTACATTCCATTGCTACATCTACGGATTTAAATGCTA
TTGTCTACCAAAGAGATAATGTCGTCATGAATCTAGACACTATAGAAAAATTAGTAGAAATACCACAAATAGTAGGTTTT
AAAGATGGACATGGAAGTATGGAACATATTATTGAATTCACACAAAGTATCGGTAACAAAATCGGTTGGTTAAACGGAAT
GCCAATGGCGGAAGTGACAATGCCAGCTTATATTCCATTAGGATTTGATTCTTATTCATCCGCTATTTCCAACTATATCC
CTCATATTTCAAGAAAATTTTACGATGCATTACTGGAAGGGGATGAAACAACGGTTAATGAAATTTACCAAGAAGTCATT
CTCCCTATTAACCGAATTCGCAGACAAGGGAAAGGATATGCGATTTCTTTGATTAAGGCAGGCATGGAAATTGTTGGTTT
ACCTATAAACAGTAATAATGTGCGTCCACCTGTAACACCTGTAAAGAAAGAGCATTATGAACAACTGGAGGAAATCCTAA
AGAAAGCATTAATTAAATACCCTTCTAATCTAACGAAAAGCCTTTAA

Upstream 100 bases:

>100_bases
ATTCGAAGCGCATCGTGCCATGTATATCCATCTGGATCATGTACGAAAGCGTGTGAAATTAGGAAAAACAAATAATGAAG
AAAATTAGAGGAGGATTTTT

Downstream 100 bases:

>100_bases
TAAATTCAATATAGAAAGGAAGATGGATATGTTAAAAACAGATAAACAGTCATACTTGAATTTCATAAATGGGAGATGGA
AGTCTTCTGAAGGTGGAGAA

Product: 5-dehydro-4-deoxyglucarate dehydratase

Products: NA

Alternate protein names: 5-keto-4-deoxy-glucarate dehydratase; KDGDH

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNRKAPTGILGFPVTPFDNQGNIDELALSQNIKYLLDSGLEAIFVACGAGEYHSLENGEYESIIEIATSTVKGQVPVYTG
VGGNLSDAVHKAKLSEKHGVDGYLIMPAYLINGEQEGIYQYVHSIATSTDLNAIVYQRDNVVMNLDTIEKLVEIPQIVGF
KDGHGSMEHIIEFTQSIGNKIGWLNGMPMAEVTMPAYIPLGFDSYSSAISNYIPHISRKFYDALLEGDETTVNEIYQEVI
LPINRIRRQGKGYAISLIKAGMEIVGLPINSNNVRPPVTPVKKEHYEQLEEILKKALIKYPSNLTKSL

Sequences:

>Translated_308_residues
MNRKAPTGILGFPVTPFDNQGNIDELALSQNIKYLLDSGLEAIFVACGAGEYHSLENGEYESIIEIATSTVKGQVPVYTG
VGGNLSDAVHKAKLSEKHGVDGYLIMPAYLINGEQEGIYQYVHSIATSTDLNAIVYQRDNVVMNLDTIEKLVEIPQIVGF
KDGHGSMEHIIEFTQSIGNKIGWLNGMPMAEVTMPAYIPLGFDSYSSAISNYIPHISRKFYDALLEGDETTVNEIYQEVI
LPINRIRRQGKGYAISLIKAGMEIVGLPINSNNVRPPVTPVKKEHYEQLEEILKKALIKYPSNLTKSL
>Mature_308_residues
MNRKAPTGILGFPVTPFDNQGNIDELALSQNIKYLLDSGLEAIFVACGAGEYHSLENGEYESIIEIATSTVKGQVPVYTG
VGGNLSDAVHKAKLSEKHGVDGYLIMPAYLINGEQEGIYQYVHSIATSTDLNAIVYQRDNVVMNLDTIEKLVEIPQIVGF
KDGHGSMEHIIEFTQSIGNKIGWLNGMPMAEVTMPAYIPLGFDSYSSAISNYIPHISRKFYDALLEGDETTVNEIYQEVI
LPINRIRRQGKGYAISLIKAGMEIVGLPINSNNVRPPVTPVKKEHYEQLEEILKKALIKYPSNLTKSL

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Escherichia coli, GI1788823, Length=294, Percent_Identity=24.1496598639456, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082415, Length=295, Percent_Identity=25.0847457627119, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786463, Length=283, Percent_Identity=23.321554770318, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789620, Length=295, Percent_Identity=21.0169491525424, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): KDGD_OCEIH (Q8EMK1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693763.1
- ProteinModelPortal:   Q8EMK1
- GeneID:   1016003
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2841
- NMPDR:   fig|221109.1.peg.2844
- HOGENOM:   HBG358848
- OMA:   LPHYLID
- ProtClustDB:   PRK03620
- BioCyc:   OIHE221109:OB2841-MONOMER
- BRENDA:   4.2.1.41
- HAMAP:   MF_00694
- InterPro:   IPR013785
- InterPro:   IPR017655
- InterPro:   IPR002220
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- TIGRFAMs:   TIGR03249

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.41

Molecular weight: Translated: 34005; Mature: 34005

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKAPTGILGFPVTPFDNQGNIDELALSQNIKYLLDSGLEAIFVACGAGEYHSLENGEY
CCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
ESIIEIATSTVKGQVPVYTGVGGNLSDAVHKAKLSEKHGVDGYLIMPAYLINGEQEGIYQ
HHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCEEEECEEEECCCHHHHHH
YVHSIATSTDLNAIVYQRDNVVMNLDTIEKLVEIPQIVGFKDGHGSMEHIIEFTQSIGNK
HHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
IGWLNGMPMAEVTMPAYIPLGFDSYSSAISNYIPHISRKFYDALLEGDETTVNEIYQEVI
CCCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LPINRIRRQGKGYAISLIKAGMEIVGLPINSNNVRPPVTPVKKEHYEQLEEILKKALIKY
HHHHHHHHCCCCEEEHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
PSNLTKSL
CCHHHHCC
>Mature Secondary Structure
MNRKAPTGILGFPVTPFDNQGNIDELALSQNIKYLLDSGLEAIFVACGAGEYHSLENGEY
CCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCH
ESIIEIATSTVKGQVPVYTGVGGNLSDAVHKAKLSEKHGVDGYLIMPAYLINGEQEGIYQ
HHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCEEEECEEEECCCHHHHHH
YVHSIATSTDLNAIVYQRDNVVMNLDTIEKLVEIPQIVGFKDGHGSMEHIIEFTQSIGNK
HHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
IGWLNGMPMAEVTMPAYIPLGFDSYSSAISNYIPHISRKFYDALLEGDETTVNEIYQEVI
CCCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LPINRIRRQGKGYAISLIKAGMEIVGLPINSNNVRPPVTPVKKEHYEQLEEILKKALIKY
HHHHHHHHCCCCEEEHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
PSNLTKSL
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376