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The map label for this gene is kduI

Identifier: 23100268

GI number: 23100268

Start: 2892855

End: 2893688

Strand: Reverse

Name: kduI

Synonym: OB2813

Alternate gene names: 23100268

Gene position: 2893688-2892855 (Counterclockwise)

Preceding gene: 23100269

Following gene: 23100267

Centisome position: 79.7

GC content: 31.53

Gene sequence:

>834_bases
ATGGAAATAAGATATGCAACAAATCCAAGAGATTTTAAAAATTACGATAATGAGCGAGTTAGAGAGGATTTCTTAATAAA
CAATCTTTTTGAAAAAGGAAATATAAATATGGTCTATTCTCACTATGACCGTTTAATTGTTGGAGGTGCTGTCCCTACAA
ATTCACCTTTACATTTGGAAGACAAAGAAACATTGAAAACAGAATACTTTTTAGAGAGAAGAGAGGTTGGCTTCTTTAAT
ATTTCAAATAATAAAGGGAAAATCACTGTGGATGGTGAAGTATATGAATTAGATCATCGAGATTGTTTATATGTAGGAAA
AGAAAATAAAAACATTTTAATTGAAAGTATAGATTCAGATAACCCAGCAAGATTTTATATTGTTTCTGCAACAGCTCATA
AGAACTATCCTACTCAAAAAAAATCACTACAAGAAGCAACTCATAGACATTTGGGAGATGACTCTCAGTCGAATAAACGA
GATCTTTATCAATATATACATGCAGATGGAATCCAAAGTTGCCAATTAATGATGGGAATGACTTTCTTATCGGAAAATAA
TATGTGGAATACAATGCCACCACATATTCATGACAGAAGAATGGAAGCTTATTTATATTTTGATATAGACGAAGATGAAA
AGATTATGCATTTTATGGGACAACCAAATGATACAAGAAATATTGTAGTATCAAATGAACAAGCGGTACTCTCACCACCA
TGGTCTATTCACTCAGGTGTAGGTACTAAAAATTATACATTTATTTGGGCGATGGCTGGTGAAAATTATACATTTGATGA
TATGGATCATGTGGTTAAAAACCAGCTAAAATAG

Upstream 100 bases:

>100_bases
ATTTTTAGCATCCAGTGCATCTGATTATATGAATGGATTTACTATAGCAGTGGATGGCGGATATTTAGGAAGATAAAAAT
AGTAGAAAGGGGAATGTAAA

Downstream 100 bases:

>100_bases
TAAGTAACTATTCTGGATAAAAGAATAGTTGTTGGTATCAACTATAATAATCGGGAGAATAAATAGTTCACACGAATAGG
TGTGGACTATTTTATTTGTA

Product: 5-keto-4-deoxyuronate isomerase

Products: NA

Alternate protein names: 5-keto-4-deoxyuronate isomerase; DKI isomerase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEIRYATNPRDFKNYDNERVREDFLINNLFEKGNINMVYSHYDRLIVGGAVPTNSPLHLEDKETLKTEYFLERREVGFFN
ISNNKGKITVDGEVYELDHRDCLYVGKENKNILIESIDSDNPARFYIVSATAHKNYPTQKKSLQEATHRHLGDDSQSNKR
DLYQYIHADGIQSCQLMMGMTFLSENNMWNTMPPHIHDRRMEAYLYFDIDEDEKIMHFMGQPNDTRNIVVSNEQAVLSPP
WSIHSGVGTKNYTFIWAMAGENYTFDDMDHVVKNQLK

Sequences:

>Translated_277_residues
MEIRYATNPRDFKNYDNERVREDFLINNLFEKGNINMVYSHYDRLIVGGAVPTNSPLHLEDKETLKTEYFLERREVGFFN
ISNNKGKITVDGEVYELDHRDCLYVGKENKNILIESIDSDNPARFYIVSATAHKNYPTQKKSLQEATHRHLGDDSQSNKR
DLYQYIHADGIQSCQLMMGMTFLSENNMWNTMPPHIHDRRMEAYLYFDIDEDEKIMHFMGQPNDTRNIVVSNEQAVLSPP
WSIHSGVGTKNYTFIWAMAGENYTFDDMDHVVKNQLK
>Mature_277_residues
MEIRYATNPRDFKNYDNERVREDFLINNLFEKGNINMVYSHYDRLIVGGAVPTNSPLHLEDKETLKTEYFLERREVGFFN
ISNNKGKITVDGEVYELDHRDCLYVGKENKNILIESIDSDNPARFYIVSATAHKNYPTQKKSLQEATHRHLGDDSQSNKR
DLYQYIHADGIQSCQLMMGMTFLSENNMWNTMPPHIHDRRMEAYLYFDIDEDEKIMHFMGQPNDTRNIVVSNEQAVLSPP
WSIHSGVGTKNYTFIWAMAGENYTFDDMDHVVKNQLK

Specific function: Pectin degradation. [C]

COG id: COG3717

COG function: function code G; 5-keto 4-deoxyuronate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kduI family

Homologues:

Organism=Escherichia coli, GI1789209, Length=279, Percent_Identity=49.8207885304659, Blast_Score=295, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDUI_OCEIH (Q8EMM9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693735.1
- ProteinModelPortal:   Q8EMM9
- SMR:   Q8EMM9
- GeneID:   1016958
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2813
- NMPDR:   fig|221109.1.peg.2815
- HOGENOM:   HBG301328
- OMA:   MPAHTHD
- ProtClustDB:   PRK00924
- BioCyc:   OIHE221109:OB2813-MONOMER
- BRENDA:   5.3.1.17
- HAMAP:   MF_00687
- InterPro:   IPR011051
- InterPro:   IPR007045
- InterPro:   IPR021120
- PIRSF:   PIRSF006625

Pfam domain/function: PF04962 KduI; SSF51182 RmlC_like_cupin

EC number: =5.3.1.17

Molecular weight: Translated: 32409; Mature: 32409

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRYATNPRDFKNYDNERVREDFLINNLFEKGNINMVYSHYDRLIVGGAVPTNSPLHLE
CEEEECCCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCEEEC
DKETLKTEYFLERREVGFFNISNNKGKITVDGEVYELDHRDCLYVGKENKNILIESIDSD
CHHHHHHHHHHHHHCCEEEEECCCCCEEEECCEEEEECCCCEEEECCCCCEEEEEECCCC
NPARFYIVSATAHKNYPTQKKSLQEATHRHLGDDSQSNKRDLYQYIHADGIQSCQLMMGM
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
TFLSENNMWNTMPPHIHDRRMEAYLYFDIDEDEKIMHFMGQPNDTRNIVVSNEQAVLSPP
HEECCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHCCCCCCCCEEEEECCCEEECCC
WSIHSGVGTKNYTFIWAMAGENYTFDDMDHVVKNQLK
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEIRYATNPRDFKNYDNERVREDFLINNLFEKGNINMVYSHYDRLIVGGAVPTNSPLHLE
CEEEECCCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCEEEC
DKETLKTEYFLERREVGFFNISNNKGKITVDGEVYELDHRDCLYVGKENKNILIESIDSD
CHHHHHHHHHHHHHCCEEEEECCCCCEEEECCEEEEECCCCEEEECCCCCEEEEEECCCC
NPARFYIVSATAHKNYPTQKKSLQEATHRHLGDDSQSNKRDLYQYIHADGIQSCQLMMGM
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
TFLSENNMWNTMPPHIHDRRMEAYLYFDIDEDEKIMHFMGQPNDTRNIVVSNEQAVLSPP
HEECCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHCCCCCCCCEEEEECCCEEECCC
WSIHSGVGTKNYTFIWAMAGENYTFDDMDHVVKNQLK
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376