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The map label for this gene is sigK [H]

Identifier: 23100115

GI number: 23100115

Start: 2728296

End: 2728859

Strand: Direct

Name: sigK [H]

Synonym: OB2660

Alternate gene names: 23100115

Gene position: 2728296-2728859 (Clockwise)

Preceding gene: 23100114

Following gene: 23100116

Centisome position: 75.15

GC content: 32.45

Gene sequence:

>564_bases
ATGCGAATAGATGACACACAACTATTTCGGCGTATTCAGGAAAAAGATGAAGAAGCGTTAGAGCAATTATATGATCGCTA
TGAAAAACTACTCTTTTCATTTTCTCTTCGAATGACGAACAATGAAACAATCGCAGAAGAGGCTGTTCAAGATGTTTTTA
CAAAGTTATGGACAAAAAAACTTCCATATAAAAAGGAAAAAGGAAAGTTCTCTTCTTGGCTACTAACTATTACGAGGAAC
GCTTGTTTAGATTTAATTAGAAAAACAAAGACAGACAATGTTGAATTCCAAGAAAGAGATAGCTTAAAAGAAGACAATGA
ACCTACACCAGAAGAACATGTAGAATGGAATGAAAAAAGACAACAAATCAAATCTGCAATGCAGAGTTTAAATGATGAAC
AAAAAGAAATTATCGAATTATTTTATTTTAAAGGATTATCACAATCCAAAATATCTGAAGCATGCAATATACCTTTAGGA
ACAGTGAAAGGAAGAGTACGATTAGCGCTAAACCATCTAAACAAACAAATGGAAAATACGAATGAAAGGGGGATTCAATC
ATGA

Upstream 100 bases:

>100_bases
ATGATCCAAAGCATGTAAAATGTCCGTTATCTATTTAAACATTTTATATACCTCCTTACTCTATGGTATCTTTAAATCAA
ACAACTAAAAGGAGAATCAG

Downstream 100 bases:

>100_bases
CAAAGCTTACTTGCGATAATTTAATCGATTATATTAATCATCAATTAACCGAAGAAGAAAGAAAATCTTTTGAACAGCAT
CTTGAAGAATGCGAAGAATG

Product: RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MRIDDTQLFRRIQEKDEEALEQLYDRYEKLLFSFSLRMTNNETIAEEAVQDVFTKLWTKKLPYKKEKGKFSSWLLTITRN
ACLDLIRKTKTDNVEFQERDSLKEDNEPTPEEHVEWNEKRQQIKSAMQSLNDEQKEIIELFYFKGLSQSKISEACNIPLG
TVKGRVRLALNHLNKQMENTNERGIQS

Sequences:

>Translated_187_residues
MRIDDTQLFRRIQEKDEEALEQLYDRYEKLLFSFSLRMTNNETIAEEAVQDVFTKLWTKKLPYKKEKGKFSSWLLTITRN
ACLDLIRKTKTDNVEFQERDSLKEDNEPTPEEHVEWNEKRQQIKSAMQSLNDEQKEIIELFYFKGLSQSKISEACNIPLG
TVKGRVRLALNHLNKQMENTNERGIQS
>Mature_187_residues
MRIDDTQLFRRIQEKDEEALEQLYDRYEKLLFSFSLRMTNNETIAEEAVQDVFTKLWTKKLPYKKEKGKFSSWLLTITRN
ACLDLIRKTKTDNVEFQERDSLKEDNEPTPEEHVEWNEKRQQIKSAMQSLNDEQKEIIELFYFKGLSQSKISEACNIPLG
TVKGRVRLALNHLNKQMENTNERGIQS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 22194; Mature: 22194

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDDTQLFRRIQEKDEEALEQLYDRYEKLLFSFSLRMTNNETIAEEAVQDVFTKLWTKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
LPYKKEKGKFSSWLLTITRNACLDLIRKTKTDNVEFQERDSLKEDNEPTPEEHVEWNEKR
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHCCHHHHH
QQIKSAMQSLNDEQKEIIELFYFKGLSQSKISEACNIPLGTVKGRVRLALNHLNKQMENT
HHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NERGIQS
CCCCCCC
>Mature Secondary Structure
MRIDDTQLFRRIQEKDEEALEQLYDRYEKLLFSFSLRMTNNETIAEEAVQDVFTKLWTKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
LPYKKEKGKFSSWLLTITRNACLDLIRKTKTDNVEFQERDSLKEDNEPTPEEHVEWNEKR
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHCCHHHHH
QQIKSAMQSLNDEQKEIIELFYFKGLSQSKISEACNIPLGTVKGRVRLALNHLNKQMENT
HHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NERGIQS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA