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The map label for this gene is nplT [H]

Identifier: 23100016

GI number: 23100016

Start: 2618420

End: 2620171

Strand: Direct

Name: nplT [H]

Synonym: OB2561

Alternate gene names: 23100016

Gene position: 2618420-2620171 (Clockwise)

Preceding gene: 23100004

Following gene: 23100033

Centisome position: 72.12

GC content: 33.9

Gene sequence:

>1752_bases
ATGCTAAAAGAAGCAGTATTTCATCGACCAAAGAATAATTATGCTTACCTTTACAAAGAGAATACGTTACACATTCGTTT
ACAAACAAAGAAAGATGATGTAGTAAAAGCCACACTTATCTATGCAGATCCTTATAATTGGGATGATTCAGGATGGATTT
ATAACAAGGCAGACATGCGTCTCATAGCTTCCGATCAGCTATTCGATTATTGGCAAATTGAAATTTATTGTAAAACACGT
CGAATAAGATATGGATTTCAATTATCTGATACTAAAGAAACTCTTATTTATACAGAGAAAGGATTTTATAAAGAAATTCC
AAATGATGACACTGCTTATTACTTCTGCTTCCCTTTTTTAAACCCAATAGATCAATTTCAAGCACCAGAATGGGTAAAGG
AAACTGTATGGTATCAAATTTTTCCTGAACGATTTGCGAACGGAAATGAAACAATTAATCCTCCTGGAACTTTACCTTGG
GGAAGCACTACCCCTACACCTACAGATTTTTTTGGTGGTGACTTTCAAGGTATAATCAATCACTTGGATTATCTTGTAGA
TTTAGGTATTTCCGGTATTTATTTTACGCCTATATTTAAAGCATTTTCTAACCATAAATACGATACGATTGACTATATGG
AAATCGATCCTCAATTTGGGGATAAACAGACATTTCGAAAGTTAGTTAAAGCTTGTCATCAACGAGGAATTCGCATTATG
CTTGATGCTGTATTTAATCATAGTGGCTATTTCTTTGAACCATTTCAGGATGTGTTAAAAAACCAAGAGAAGTCTATTTA
TAAGGAATGGTTTCATATCGAGAGTTTTCCTTTGAGAACAGACCCAGCACCAAACTATGACACCTTTGCATATGTAGGTT
CAATGCCAAAACTAAATACAGAGCACCCGGAAGTGAAATCATATTTACTTAAAGTAGCATCATATTGGATTGAAGAGTTT
GATATCGATGGGTGGCGATTAGACGTAGCTAATGAAGTGGATCACCAATTTTGGAGAGATTTCCGTAGACAAGTGAAATC
CATAAAGCCAGATGTCTATATTCTCGGAGAAATATGGCATGATTCGATGCCCTGGTTACAAGGAGATCAATTTGACGCAG
TAATGAACTATCCACTTACCAGTGCTGCACTTGATTTTATTGCAAAGGAAAAAATAGATGTTAAAGAATTTGTCCAACAA
ATTTCTAATGTTCTACTTTCTTATCCACAGAACGTGAATGAAGTAGCATTTAATCTATTAGGCAGTCATGATACTCCCAG
AGTTTTAACACATTGCAACAATCAAGTCGAAAAAGCAAAACTTCTATATATATTATTATTAACGATGCCAGGTTCTCCTT
GTATTTATTATGGAGATGAAATTGGAATGACTGGAGAAGGGGATCCTGGTTGCAGAGAATGTATGGTTTGGGACGAGAAT
GAACAAAATGTAGAATTAAAATCTTTCCTTAAAAAATTAATTCATGTGCGGAAAACGGAAAAGGCTGTTGCAAATCAAGG
AGAATTTTCATTTGTAAAAACTAGTGATAATTATTTCATTTATAAACTTTTTCAACAGCATATGGAAATTTATATTATTA
TAAACCCAAGTAATCAGGCTCTCGAAGTATCACTTCCGGTAACCAAGTGGAACAGTGCCTTCGATTTATGGGAGGAAGAT
GATTTCAATATTAACACTCCAATAAAATGTGAGGAAAATAGTTTCAAAATAATCAAAGTAAATTATTCATAA

Upstream 100 bases:

>100_bases
AATTTAAGGGCAATGCTTTGTAATTACACAACAACCCCGGTTTCCAGCAGTACCACGTTGGAAGTCGGGGTTATTTTATT
TTTATAGGAGGAGAAAACTG

Downstream 100 bases:

>100_bases
CCGGTATATTTTTTTCAAAGAGGCTGGGAATTTAAAGATAAGTTTTAACTTTACCATAGGCGGCGACTTCAGCGGGGATG
GGAGCTTTTTATATTGACGG

Product: cyclomaltodextrinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MLKEAVFHRPKNNYAYLYKENTLHIRLQTKKDDVVKATLIYADPYNWDDSGWIYNKADMRLIASDQLFDYWQIEIYCKTR
RIRYGFQLSDTKETLIYTEKGFYKEIPNDDTAYYFCFPFLNPIDQFQAPEWVKETVWYQIFPERFANGNETINPPGTLPW
GSTTPTPTDFFGGDFQGIINHLDYLVDLGISGIYFTPIFKAFSNHKYDTIDYMEIDPQFGDKQTFRKLVKACHQRGIRIM
LDAVFNHSGYFFEPFQDVLKNQEKSIYKEWFHIESFPLRTDPAPNYDTFAYVGSMPKLNTEHPEVKSYLLKVASYWIEEF
DIDGWRLDVANEVDHQFWRDFRRQVKSIKPDVYILGEIWHDSMPWLQGDQFDAVMNYPLTSAALDFIAKEKIDVKEFVQQ
ISNVLLSYPQNVNEVAFNLLGSHDTPRVLTHCNNQVEKAKLLYILLLTMPGSPCIYYGDEIGMTGEGDPGCRECMVWDEN
EQNVELKSFLKKLIHVRKTEKAVANQGEFSFVKTSDNYFIYKLFQQHMEIYIIINPSNQALEVSLPVTKWNSAFDLWEED
DFNINTPIKCEENSFKIIKVNYS

Sequences:

>Translated_583_residues
MLKEAVFHRPKNNYAYLYKENTLHIRLQTKKDDVVKATLIYADPYNWDDSGWIYNKADMRLIASDQLFDYWQIEIYCKTR
RIRYGFQLSDTKETLIYTEKGFYKEIPNDDTAYYFCFPFLNPIDQFQAPEWVKETVWYQIFPERFANGNETINPPGTLPW
GSTTPTPTDFFGGDFQGIINHLDYLVDLGISGIYFTPIFKAFSNHKYDTIDYMEIDPQFGDKQTFRKLVKACHQRGIRIM
LDAVFNHSGYFFEPFQDVLKNQEKSIYKEWFHIESFPLRTDPAPNYDTFAYVGSMPKLNTEHPEVKSYLLKVASYWIEEF
DIDGWRLDVANEVDHQFWRDFRRQVKSIKPDVYILGEIWHDSMPWLQGDQFDAVMNYPLTSAALDFIAKEKIDVKEFVQQ
ISNVLLSYPQNVNEVAFNLLGSHDTPRVLTHCNNQVEKAKLLYILLLTMPGSPCIYYGDEIGMTGEGDPGCRECMVWDEN
EQNVELKSFLKKLIHVRKTEKAVANQGEFSFVKTSDNYFIYKLFQQHMEIYIIINPSNQALEVSLPVTKWNSAFDLWEED
DFNINTPIKCEENSFKIIKVNYS
>Mature_583_residues
MLKEAVFHRPKNNYAYLYKENTLHIRLQTKKDDVVKATLIYADPYNWDDSGWIYNKADMRLIASDQLFDYWQIEIYCKTR
RIRYGFQLSDTKETLIYTEKGFYKEIPNDDTAYYFCFPFLNPIDQFQAPEWVKETVWYQIFPERFANGNETINPPGTLPW
GSTTPTPTDFFGGDFQGIINHLDYLVDLGISGIYFTPIFKAFSNHKYDTIDYMEIDPQFGDKQTFRKLVKACHQRGIRIM
LDAVFNHSGYFFEPFQDVLKNQEKSIYKEWFHIESFPLRTDPAPNYDTFAYVGSMPKLNTEHPEVKSYLLKVASYWIEEF
DIDGWRLDVANEVDHQFWRDFRRQVKSIKPDVYILGEIWHDSMPWLQGDQFDAVMNYPLTSAALDFIAKEKIDVKEFVQQ
ISNVLLSYPQNVNEVAFNLLGSHDTPRVLTHCNNQVEKAKLLYILLLTMPGSPCIYYGDEIGMTGEGDPGCRECMVWDEN
EQNVELKSFLKKLIHVRKTEKAVANQGEFSFVKTSDNYFIYKLFQQHMEIYIIINPSNQALEVSLPVTKWNSAFDLWEED
DFNINTPIKCEENSFKIIKVNYS

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=393, Percent_Identity=23.9185750636132, Blast_Score=94, Evalue=5e-19,
Organism=Escherichia coli, GI1786604, Length=525, Percent_Identity=30.2857142857143, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1790687, Length=391, Percent_Identity=27.8772378516624, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789995, Length=488, Percent_Identity=23.7704918032787, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322245, Length=407, Percent_Identity=20.3931203931204, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321726, Length=407, Percent_Identity=21.6216216216216, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=405, Percent_Identity=20.2469135802469, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=405, Percent_Identity=20, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=228, Percent_Identity=23.2456140350877, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=228, Percent_Identity=23.2456140350877, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=228, Percent_Identity=23.2456140350877, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45549022, Length=410, Percent_Identity=24.390243902439, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24586587, Length=409, Percent_Identity=24.9388753056235, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583745, Length=406, Percent_Identity=25.3694581280788, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24586597, Length=407, Percent_Identity=22.1130221130221, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221330053, Length=182, Percent_Identity=27.4725274725275, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586599, Length=225, Percent_Identity=26.2222222222222, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24583747, Length=228, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583749, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 68554; Mature: 68554

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEAVFHRPKNNYAYLYKENTLHIRLQTKKDDVVKATLIYADPYNWDDSGWIYNKADMR
CCCHHHHCCCCCCEEEEEECCEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEECCCEE
LIASDQLFDYWQIEIYCKTRRIRYGFQLSDTKETLIYTEKGFYKEIPNDDTAYYFCFPFL
EEECCCCCEEEEEEEEEEEEEEEECEEECCCCEEEEEECCCCHHCCCCCCCEEEEEECCC
NPIDQFQAPEWVKETVWYQIFPERFANGNETINPPGTLPWGSTTPTPTDFFGGDFQGIIN
CHHHHHCCCHHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
HLDYLVDLGISGIYFTPIFKAFSNHKYDTIDYMEIDPQFGDKQTFRKLVKACHQRGIRIM
HHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
LDAVFNHSGYFFEPFQDVLKNQEKSIYKEWFHIESFPLRTDPAPNYDTFAYVGSMPKLNT
EEEHHCCCCCEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCC
EHPEVKSYLLKVASYWIEEFDIDGWRLDVANEVDHQFWRDFRRQVKSIKPDVYILGEIWH
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DSMPWLQGDQFDAVMNYPLTSAALDFIAKEKIDVKEFVQQISNVLLSYPQNVNEVAFNLL
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
GSHDTPRVLTHCNNQVEKAKLLYILLLTMPGSPCIYYGDEIGMTGEGDPGCRECMVWDEN
CCCCCCHHHHHCCCHHHHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEECCC
EQNVELKSFLKKLIHVRKTEKAVANQGEFSFVKTSDNYFIYKLFQQHMEIYIIINPSNQA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEHHHCCEEEEEECCCCCE
LEVSLPVTKWNSAFDLWEEDDFNINTPIKCEENSFKIIKVNYS
EEEEECCEECCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECC
>Mature Secondary Structure
MLKEAVFHRPKNNYAYLYKENTLHIRLQTKKDDVVKATLIYADPYNWDDSGWIYNKADMR
CCCHHHHCCCCCCEEEEEECCEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEECCCEE
LIASDQLFDYWQIEIYCKTRRIRYGFQLSDTKETLIYTEKGFYKEIPNDDTAYYFCFPFL
EEECCCCCEEEEEEEEEEEEEEEECEEECCCCEEEEEECCCCHHCCCCCCCEEEEEECCC
NPIDQFQAPEWVKETVWYQIFPERFANGNETINPPGTLPWGSTTPTPTDFFGGDFQGIIN
CHHHHHCCCHHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
HLDYLVDLGISGIYFTPIFKAFSNHKYDTIDYMEIDPQFGDKQTFRKLVKACHQRGIRIM
HHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
LDAVFNHSGYFFEPFQDVLKNQEKSIYKEWFHIESFPLRTDPAPNYDTFAYVGSMPKLNT
EEEHHCCCCCEECHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCC
EHPEVKSYLLKVASYWIEEFDIDGWRLDVANEVDHQFWRDFRRQVKSIKPDVYILGEIWH
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DSMPWLQGDQFDAVMNYPLTSAALDFIAKEKIDVKEFVQQISNVLLSYPQNVNEVAFNLL
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
GSHDTPRVLTHCNNQVEKAKLLYILLLTMPGSPCIYYGDEIGMTGEGDPGCRECMVWDEN
CCCCCCHHHHHCCCHHHHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEECCC
EQNVELKSFLKKLIHVRKTEKAVANQGEFSFVKTSDNYFIYKLFQQHMEIYIIINPSNQA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEHHHCCEEEEEECCCCCE
LEVSLPVTKWNSAFDLWEEDDFNINTPIKCEENSFKIIKVNYS
EEEEECCEECCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]