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The map label for this gene is clpP

Identifier: 23099911

GI number: 23099911

Start: 2513422

End: 2514003

Strand: Direct

Name: clpP

Synonym: OB2456

Alternate gene names: 23099911

Gene position: 2513422-2514003 (Clockwise)

Preceding gene: 23099910

Following gene: 23099976

Centisome position: 69.23

GC content: 36.6

Gene sequence:

>582_bases
ATGAATTTAGTACCTACAGTTATTGAACAAACTAACCGTGGAGAGCGCGCATATGATATTTACTCTCGTTTACTTAAAGA
TCGTATTATCTTATTAGGAAGCGGAATTGATGATAATGTGTCCAATTCCATTGTAGCACAGCTACTTTTCTTAGAAGCTG
AAGACCCAGATAAAGATATCTCCTTATACATTAATTCACCTGGTGGTTCGATCACAGCTGGGATGGCGATTTATGATACG
ATGAATTTCATTAAGCCAAACGTATCTACTATTTGTATCGGTATGGCAGCATCTATGGGTGCTTTCCTACTTACAGCTGG
TGAAAAAGGCAAACGATTTGCTTTACCAAATAGTGAAATCATGATTCACCAACCTTTAGGTGGTACGCAAGGTCAAGCTA
CAGATATTGAAATTCATGCAAAACGTATTATTGATATTAAACGTCGTATGAATGAAATCATGGCTGAGAAAACAGGTCAA
CCTATTGAAGTTATTGAACGTGATACAGAACGTGATAATTTCATGACTGCTGATCAGTCTGTGGAATACGGCTTAATTGA
TAAAATTCTAGAACGTAAATAG

Upstream 100 bases:

>100_bases
TAAACTTTTTGTTTGACCTTTATTGACCAATAAGCTATGATAAATGTATACACATTCCATGGAAGAACTATTTCAATTGG
AAATTAAAGGAGGATGTATG

Downstream 100 bases:

>100_bases
TCATTAAAAATGTTGAGCTTACTCTAGCTCAACATTTTTTGTGTCTTTTAAGATTAATATCCTTCGCAACCTACCACGAT
TGTATCGTTGAAGAACAATA

Product: ATP-dependent Clp protease proteolytic subunit

Products: NA

Alternate protein names: Endopeptidase Clp

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MNLVPTVIEQTNRGERAYDIYSRLLKDRIILLGSGIDDNVSNSIVAQLLFLEAEDPDKDISLYINSPGGSITAGMAIYDT
MNFIKPNVSTICIGMAASMGAFLLTAGEKGKRFALPNSEIMIHQPLGGTQGQATDIEIHAKRIIDIKRRMNEIMAEKTGQ
PIEVIERDTERDNFMTADQSVEYGLIDKILERK

Sequences:

>Translated_193_residues
MNLVPTVIEQTNRGERAYDIYSRLLKDRIILLGSGIDDNVSNSIVAQLLFLEAEDPDKDISLYINSPGGSITAGMAIYDT
MNFIKPNVSTICIGMAASMGAFLLTAGEKGKRFALPNSEIMIHQPLGGTQGQATDIEIHAKRIIDIKRRMNEIMAEKTGQ
PIEVIERDTERDNFMTADQSVEYGLIDKILERK
>Mature_193_residues
MNLVPTVIEQTNRGERAYDIYSRLLKDRIILLGSGIDDNVSNSIVAQLLFLEAEDPDKDISLYINSPGGSITAGMAIYDT
MNFIKPNVSTICIGMAASMGAFLLTAGEKGKRFALPNSEIMIHQPLGGTQGQATDIEIHAKRIIDIKRRMNEIMAEKTGQ
PIEVIERDTERDNFMTADQSVEYGLIDKILERK

Specific function: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. ClpXP is involved in the complete degradation of the Site-2 clipped anti- sigma-

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family

Homologues:

Organism=Homo sapiens, GI5174419, Length=190, Percent_Identity=54.2105263157895, Blast_Score=225, Evalue=2e-59,
Organism=Escherichia coli, GI1786641, Length=193, Percent_Identity=71.5025906735751, Blast_Score=297, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17538017, Length=186, Percent_Identity=54.3010752688172, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20129427, Length=192, Percent_Identity=56.25, Blast_Score=234, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLPP_OCEIH (Q8ENM5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693377.1
- ProteinModelPortal:   Q8ENM5
- SMR:   Q8ENM5
- MEROPS:   S14.001
- GeneID:   1017205
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2456
- NMPDR:   fig|221109.1.peg.2455
- HOGENOM:   HBG558421
- OMA:   DNVLEKS
- ProtClustDB:   PRK00277
- BioCyc:   OIHE221109:OB2456-MONOMER
- BRENDA:   3.4.21.92
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00444
- InterPro:   IPR001907
- InterPro:   IPR018215
- PANTHER:   PTHR10381
- PRINTS:   PR00127
- TIGRFAMs:   TIGR00493

Pfam domain/function: PF00574 CLP_protease

EC number: =3.4.21.92

Molecular weight: Translated: 21406; Mature: 21406

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00382 CLP_PROTEASE_HIS; PS00381 CLP_PROTEASE_SER

Important sites: ACT_SITE 98-98 ACT_SITE 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVPTVIEQTNRGERAYDIYSRLLKDRIILLGSGIDDNVSNSIVAQLLFLEAEDPDKDI
CCCCCHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHEECCCCCCCE
SLYINSPGGSITAGMAIYDTMNFIKPNVSTICIGMAASMGAFLLTAGEKGKRFALPNSEI
EEEEECCCCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEECCCCCCEEECCCCCE
MIHQPLGGTQGQATDIEIHAKRIIDIKRRMNEIMAEKTGQPIEVIERDTERDNFMTADQS
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEECCCCC
VEYGLIDKILERK
CCHHHHHHHHHCC
>Mature Secondary Structure
MNLVPTVIEQTNRGERAYDIYSRLLKDRIILLGSGIDDNVSNSIVAQLLFLEAEDPDKDI
CCCCCHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHEECCCCCCCE
SLYINSPGGSITAGMAIYDTMNFIKPNVSTICIGMAASMGAFLLTAGEKGKRFALPNSEI
EEEEECCCCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEECCCCCCEEECCCCCE
MIHQPLGGTQGQATDIEIHAKRIIDIKRRMNEIMAEKTGQPIEVIERDTERDNFMTADQS
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEECCCCC
VEYGLIDKILERK
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376