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The map label for this gene is aroK

Identifier: 23099910

GI number: 23099910

Start: 2512190

End: 2512741

Strand: Direct

Name: aroK

Synonym: OB2455

Alternate gene names: 23099910

Gene position: 2512190-2512741 (Clockwise)

Preceding gene: 23099909

Following gene: 23099911

Centisome position: 69.2

GC content: 32.25

Gene sequence:

>552_bases
ATGGGGAAGTTTCCATTAAGAGAACAAAGTATAGTTTTAATAGGTTTTATGGGCGTTGGAAAAACGACGATTGGTAAGAC
GGTAGCAAAAAAATTATACCGTGATTTTATAGATATTGATGAAGAAATCGAAAAAAGATTCAAGATGCCAACTAAAGATA
TTTTTCGATTATATGGAGAAAAAACATTTCGCAATGCGGAAAAAGATATTATATCTGAAATATGCCAACAACGATTAAAA
GTTGTTTCTTTAGGTGGAGGAGCATTTTTACAAAAGGAAATACAAGAAATTTGTTTAAACTCTAGTATTATATTTTATCT
CGATTTGTCTTGGGATCGTTGGAAAGACCGTATCGAACTTTTAATGGAAAGTCGACCAGTACTTCAAGGCAAGACTATGA
AGGAAATGAAACAATTATATGAAGACAGAAAGCCAATATATGAGCAACATCATTCTAGAGTAGATACAGATTATCATGAG
ATAGAAGAAGTTGCCGATTATATCGCCGAATCACTAAAATTAGCATGGGATATATATGAACCTACTTCATGA

Upstream 100 bases:

>100_bases
AAACTTGCAAAAAAAGCCATTCAGCATCATCCTTAAAATTCGTGCAAACTTAGATTTATTCCATTATAATAACGTAAAGA
TTGTATGTAGGAGGAACAAC

Downstream 100 bases:

>100_bases
GAAAGCTTACCTATTCTGGTTAATTACTTTAGTTATAGCCATCATAGTACCAATATTTATATGGACTAAAACAAAGCAAG
CATCAAAAGAAAAAGTCAAC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MGKFPLREQSIVLIGFMGVGKTTIGKTVAKKLYRDFIDIDEEIEKRFKMPTKDIFRLYGEKTFRNAEKDIISEICQQRLK
VVSLGGGAFLQKEIQEICLNSSIIFYLDLSWDRWKDRIELLMESRPVLQGKTMKEMKQLYEDRKPIYEQHHSRVDTDYHE
IEEVADYIAESLKLAWDIYEPTS

Sequences:

>Translated_183_residues
MGKFPLREQSIVLIGFMGVGKTTIGKTVAKKLYRDFIDIDEEIEKRFKMPTKDIFRLYGEKTFRNAEKDIISEICQQRLK
VVSLGGGAFLQKEIQEICLNSSIIFYLDLSWDRWKDRIELLMESRPVLQGKTMKEMKQLYEDRKPIYEQHHSRVDTDYHE
IEEVADYIAESLKLAWDIYEPTS
>Mature_182_residues
GKFPLREQSIVLIGFMGVGKTTIGKTVAKKLYRDFIDIDEEIEKRFKMPTKDIFRLYGEKTFRNAEKDIISEICQQRLKV
VSLGGGAFLQKEIQEICLNSSIIFYLDLSWDRWKDRIELLMESRPVLQGKTMKEMKQLYEDRKPIYEQHHSRVDTDYHEI
EEVADYIAESLKLAWDIYEPTS

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=163, Percent_Identity=31.2883435582822, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786587, Length=172, Percent_Identity=27.3255813953488, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_OCEIH (Q8ENM6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693376.1
- ProteinModelPortal:   Q8ENM6
- SMR:   Q8ENM6
- GeneID:   1017208
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2455
- NMPDR:   fig|221109.1.peg.2453
- HOGENOM:   HBG335815
- OMA:   MIISERV
- ProtClustDB:   PRK00131
- BioCyc:   OIHE221109:OB2455-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 21608; Mature: 21477

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 40-40 BINDING 64-64 BINDING 86-86 BINDING 125-125 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKFPLREQSIVLIGFMGVGKTTIGKTVAKKLYRDFIDIDEEIEKRFKMPTKDIFRLYGE
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
KTFRNAEKDIISEICQQRLKVVSLGGGAFLQKEIQEICLNSSIIFYLDLSWDRWKDRIEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
LMESRPVLQGKTMKEMKQLYEDRKPIYEQHHSRVDTDYHEIEEVADYIAESLKLAWDIYE
HHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PTS
CCC
>Mature Secondary Structure 
GKFPLREQSIVLIGFMGVGKTTIGKTVAKKLYRDFIDIDEEIEKRFKMPTKDIFRLYGE
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
KTFRNAEKDIISEICQQRLKVVSLGGGAFLQKEIQEICLNSSIIFYLDLSWDRWKDRIEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
LMESRPVLQGKTMKEMKQLYEDRKPIYEQHHSRVDTDYHEIEEVADYIAESLKLAWDIYE
HHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PTS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376