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The map label for this gene is rnhA [H]

Identifier: 23099909

GI number: 23099909

Start: 2511733

End: 2512125

Strand: Direct

Name: rnhA [H]

Synonym: OB2454

Alternate gene names: 23099909

Gene position: 2511733-2512125 (Clockwise)

Preceding gene: 23099903

Following gene: 23099910

Centisome position: 69.18

GC content: 35.37

Gene sequence:

>393_bases
ATGATTGAGGTATATACAGATGGAGCAACATTAGGGAATCCAGGCATTGGCGGAGCGGGAATCTATATAAAAGATGGCAA
ACAAAGCTATCAATATTCTATACCGCTTCCTTCTATGTCAAATCATGAAGCAGAATTCCATGCAATCATTCATGGACTAC
AAGTATGTAAGGAACATTTTCCTAACGAGATATTGTCATTTCGGACAGATTCTAAGGTAGCAGCAGACTTAATTGAAAAA
GGCCATACTAAAAATATAGTGTTTCAATCCTTATTGCATACAATAAATCAAGTCTCATCAGAGTTTCCATTCTTCTTTAT
CAAATGGATTCCTGAAAAGAAGAATTATCATGCGGATAAACTTGCAAAAAAAGCCATTCAGCATCATCCTTAA

Upstream 100 bases:

>100_bases
TATTTAGTATGTTACTAATATTTTTACACTGTTTTCATTTTATTTGAAAATCTTATATACTGTATAAGTATTTTAAAGTT
AACAGTTTGAGGTGTTCATA

Downstream 100 bases:

>100_bases
AATTCGTGCAAACTTAGATTTATTCCATTATAATAACGTAAAGATTGTATGTAGGAGGAACAACATGGGGAAGTTTCCAT
TAAGAGAACAAAGTATAGTT

Product: hypothetical protein

Products: 5'-phosphomonoester [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MIEVYTDGATLGNPGIGGAGIYIKDGKQSYQYSIPLPSMSNHEAEFHAIIHGLQVCKEHFPNEILSFRTDSKVAADLIEK
GHTKNIVFQSLLHTINQVSSEFPFFFIKWIPEKKNYHADKLAKKAIQHHP

Sequences:

>Translated_130_residues
MIEVYTDGATLGNPGIGGAGIYIKDGKQSYQYSIPLPSMSNHEAEFHAIIHGLQVCKEHFPNEILSFRTDSKVAADLIEK
GHTKNIVFQSLLHTINQVSSEFPFFFIKWIPEKKNYHADKLAKKAIQHHP
>Mature_130_residues
MIEVYTDGATLGNPGIGGAGIYIKDGKQSYQYSIPLPSMSNHEAEFHAIIHGLQVCKEHFPNEILSFRTDSKVAADLIEK
GHTKNIVFQSLLHTINQVSSEFPFFFIKWIPEKKNYHADKLAKKAIQHHP

Specific function: Not known; does not have RNase H activity [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: 3.1.26.4 [C]

Molecular weight: Translated: 14661; Mature: 14661

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVYTDGATLGNPGIGGAGIYIKDGKQSYQYSIPLPSMSNHEAEFHAIIHGLQVCKEHF
CEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
PNEILSFRTDSKVAADLIEKGHTKNIVFQSLLHTINQVSSEFPFFFIKWIPEKKNYHADK
CHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
LAKKAIQHHP
HHHHHHHCCC
>Mature Secondary Structure
MIEVYTDGATLGNPGIGGAGIYIKDGKQSYQYSIPLPSMSNHEAEFHAIIHGLQVCKEHF
CEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
PNEILSFRTDSKVAADLIEKGHTKNIVFQSLLHTINQVSSEFPFFFIKWIPEKKNYHADK
CHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
LAKKAIQHHP
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Sulfhydryl reagents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RNA-DNA hybrid; H2O [C]

Specific reaction: RNA-DNA hybrid + H2O = 5'-phosphomonoester [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: (NH4)2SO4; Dextran [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9888800 [H]