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The map label for this gene is gcvH

Identifier: 23099843

GI number: 23099843

Start: 2439612

End: 2439992

Strand: Reverse

Name: gcvH

Synonym: OB2388

Alternate gene names: 23099843

Gene position: 2439992-2439612 (Counterclockwise)

Preceding gene: 23099844

Following gene: 23099841

Centisome position: 67.21

GC content: 36.75

Gene sequence:

>381_bases
ATGAGTTTGCCACAAGATTTATTGTATTCTAAGGAGCATGAATGGGTAAAACAAGAAGATGGAAAATTACGCATTGGGAT
TACAGACTTTGCTCAAGATGAATTAGGAGACATTGTGTTTGTTGAACTTCCTGAAATTGGTGAAGAATTAAAAGTCGATG
ATCCTTTTGGTAGTGTAGAATCTGTAAAGACAGTATCTGAGCTCTATGCACCCGTAAGTGGAAAAGTAGTCGAAATCAAC
GATGACTTGGAAGATAGCCCAGAGTATGTTAATGAATCTCCATATGAAAAAGCTTGGATGATAGTGATTGAACCATCTGA
TGAAAAGGAACTTGAAGAGCTATTATCCGCAGAGAATTACGAGGCATTTATACAAGACTAA

Upstream 100 bases:

>100_bases
AGAAGATTCTTTTGAGAAGGTCTGGAAGTAAATTTTAACTAGAAAAATGATAGTATAAATTGTATAGTTAAATTGCAATG
ATAATTTGGAGGGATTTTTT

Downstream 100 bases:

>100_bases
TTTTTAGGCTGACTTTTACACGTTAATTGTGTATAGTCAGCCTTTGTTTTATCATATAATGATTATATGTAAAGGTTCTA
TGTCAAATGTGGTGGTGGGT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSLPQDLLYSKEHEWVKQEDGKLRIGITDFAQDELGDIVFVELPEIGEELKVDDPFGSVESVKTVSELYAPVSGKVVEIN
DDLEDSPEYVNESPYEKAWMIVIEPSDEKELEELLSAENYEAFIQD

Sequences:

>Translated_126_residues
MSLPQDLLYSKEHEWVKQEDGKLRIGITDFAQDELGDIVFVELPEIGEELKVDDPFGSVESVKTVSELYAPVSGKVVEIN
DDLEDSPEYVNESPYEKAWMIVIEPSDEKELEELLSAENYEAFIQD
>Mature_125_residues
SLPQDLLYSKEHEWVKQEDGKLRIGITDFAQDELGDIVFVELPEIGEELKVDDPFGSVESVKTVSELYAPVSGKVVEIND
DLEDSPEYVNESPYEKAWMIVIEPSDEKELEELLSAENYEAFIQD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=48.3050847457627, Blast_Score=125, Evalue=7e-30,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=47.4576271186441, Blast_Score=123, Evalue=5e-29,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=52.3809523809524, Blast_Score=136, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=50, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=47.8991596638655, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=42.6229508196721, Blast_Score=104, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=48.780487804878, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_OCEIH (Q8ENT9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693309.1
- ProteinModelPortal:   Q8ENT9
- SMR:   Q8ENT9
- GeneID:   1015284
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2388
- NMPDR:   fig|221109.1.peg.2385
- HOGENOM:   HBG693789
- OMA:   FCKEGEV
- ProtClustDB:   PRK01202
- BioCyc:   OIHE221109:OB2388-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14356; Mature: 14225

Theoretical pI: Translated: 3.67; Mature: 3.67

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPQDLLYSKEHEWVKQEDGKLRIGITDFAQDELGDIVFVELPEIGEELKVDDPFGSVE
CCCCHHHHHCCCCCHHHCCCCEEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPVSGKVVEINDDLEDSPEYVNESPYEKAWMIVIEPSDEKELEELLSAENY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCC
EAFIQD
EEEECC
>Mature Secondary Structure 
SLPQDLLYSKEHEWVKQEDGKLRIGITDFAQDELGDIVFVELPEIGEELKVDDPFGSVE
CCCHHHHHCCCCCHHHCCCCEEEEEECCCCHHCCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPVSGKVVEINDDLEDSPEYVNESPYEKAWMIVIEPSDEKELEELLSAENY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCC
EAFIQD
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376