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The map label for this gene is gatA2 [H]

Identifier: 23099818

GI number: 23099818

Start: 2413638

End: 2415071

Strand: Direct

Name: gatA2 [H]

Synonym: OB2363

Alternate gene names: 23099818

Gene position: 2413638-2415071 (Clockwise)

Preceding gene: 23099814

Following gene: 23099819

Centisome position: 66.48

GC content: 36.96

Gene sequence:

>1434_bases
ATGGATGATACGCAAATAATCCAAATGGATGCGACACAGCTTTCTAAGGCTATATTAAGTTCAGAACTTACAAGTGTGAA
AGCAGTTGCTGCATTTATCAAACATATTCATGAGGTAAATCCAATTATAAATGCCCTTGTAGAAGATCGGTTTATTGAAG
CAATTGAAGAGGCAAAGGAGTATGACAACCTACTAAAGAACGGACAAAAACGCGGCCCATTACACGGCGTTCCAATAAGT
ATCAAGGAGTCTCTTCATGTTACTGGACTAAAAACAACTGGAGGATTAGAACATAGACAAGATTTAATCGCTATTGAGGA
TGCAGCTGTCGTAAAGAAATTAAAAGAAGCTGGCGCCATCATTATAGGAAAAACAAATACTCCAGCACTATGCTTTTGTC
AGGAGACAGACAATAAATTATATGGTCGAACAAATAACCCATGGGATATTTCTAAATCGGCTGGTGGATCGAGCGGTGGT
GAAGGTGCATTATTAGCAGTCGGTGGAGCAGCGGTTGGCATTGGTTCTGATGTAGGAGGATCTATACGTTTTCCTGCTCA
TTTTAATGGAGTTATTGGTTTTAAACCTGGGAAAGACCAAATATCTATGGATGGGCATTTTCCAAGTATACAGCATGATT
TACAAGCACGTATGTTAACCATTGGACCAATGGGTAAGTCCGTACAAGATATGAGGTTACTATACGACATTCTAAGCCCA
TCCAATATAGAATCACAAAAATTGAAGGATTTTAAATTAGAAATCTTACCTGGTAATTCTGGGTATCCGTTATCCATTGA
AACCGTTGATATATTAAACCAATTAGAATATTTTCTAGAAAAATCATTTCCAACAAAACGAATAATGCCTCCATACTTTA
AAGATAGTGCATTAATTTGGCAAGAAATCATGTCCATCAATGGTAGTAAACTAATAGAAGATGAAGCATACAACAATGAT
CGTTCGGGAGTATATTCTTCCTTCTTAAAAGAAAAATTAACACAGAGAACATCGGTCCATCCGTATTTATCTTGGGCAAT
TATAGGTGCCAAGATGTTTAAACCATCTCATAAACGAGTGAACGAAATTTTAACAATCATTGAACAAGGCGATGGAGTGA
TAGAAACATACTTAGATAACCGTCTTTTAATTTTTCCAGTGTATCATGAAACCGCATTACCTCATGGAAAAGTATTTAAA
GAAATCTTTTCGATACGAAAGACTTACCTCCAATATATGCCTTATGTAGCATATGCGAATGTGTGGGGACTTCCTTCTTT
GACCATTCCTGTTGGGGAATCTAAAAATGGCCTACCCATTAGCATTCAAATAATGAGCAAACCTGGAAATGAGGATGCTA
TCTTTCGATTAGGCAGATTAATCGAGAAAAAGTATCGTGGCTTTAAATTAGCTCCCATACCAAACGTGGATTAG

Upstream 100 bases:

>100_bases
AAATGAGTATATAGATATCATATCATATTCTATAGGTGGAATTGTAAAATCGTGATATATTAAAAGTATCAACAATACAT
CAGCGAGGAGTAACTATATT

Downstream 100 bases:

>100_bases
AAAAAGCTCCCATTATATTTTTTCAATGGTATGCACATACTGAAAATATAGGGGAGTGTTAGAATGAAAAAACAAAAATA
CGATGAAATATCGAATGTTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MDDTQIIQMDATQLSKAILSSELTSVKAVAAFIKHIHEVNPIINALVEDRFIEAIEEAKEYDNLLKNGQKRGPLHGVPIS
IKESLHVTGLKTTGGLEHRQDLIAIEDAAVVKKLKEAGAIIIGKTNTPALCFCQETDNKLYGRTNNPWDISKSAGGSSGG
EGALLAVGGAAVGIGSDVGGSIRFPAHFNGVIGFKPGKDQISMDGHFPSIQHDLQARMLTIGPMGKSVQDMRLLYDILSP
SNIESQKLKDFKLEILPGNSGYPLSIETVDILNQLEYFLEKSFPTKRIMPPYFKDSALIWQEIMSINGSKLIEDEAYNND
RSGVYSSFLKEKLTQRTSVHPYLSWAIIGAKMFKPSHKRVNEILTIIEQGDGVIETYLDNRLLIFPVYHETALPHGKVFK
EIFSIRKTYLQYMPYVAYANVWGLPSLTIPVGESKNGLPISIQIMSKPGNEDAIFRLGRLIEKKYRGFKLAPIPNVD

Sequences:

>Translated_477_residues
MDDTQIIQMDATQLSKAILSSELTSVKAVAAFIKHIHEVNPIINALVEDRFIEAIEEAKEYDNLLKNGQKRGPLHGVPIS
IKESLHVTGLKTTGGLEHRQDLIAIEDAAVVKKLKEAGAIIIGKTNTPALCFCQETDNKLYGRTNNPWDISKSAGGSSGG
EGALLAVGGAAVGIGSDVGGSIRFPAHFNGVIGFKPGKDQISMDGHFPSIQHDLQARMLTIGPMGKSVQDMRLLYDILSP
SNIESQKLKDFKLEILPGNSGYPLSIETVDILNQLEYFLEKSFPTKRIMPPYFKDSALIWQEIMSINGSKLIEDEAYNND
RSGVYSSFLKEKLTQRTSVHPYLSWAIIGAKMFKPSHKRVNEILTIIEQGDGVIETYLDNRLLIFPVYHETALPHGKVFK
EIFSIRKTYLQYMPYVAYANVWGLPSLTIPVGESKNGLPISIQIMSKPGNEDAIFRLGRLIEKKYRGFKLAPIPNVD
>Mature_477_residues
MDDTQIIQMDATQLSKAILSSELTSVKAVAAFIKHIHEVNPIINALVEDRFIEAIEEAKEYDNLLKNGQKRGPLHGVPIS
IKESLHVTGLKTTGGLEHRQDLIAIEDAAVVKKLKEAGAIIIGKTNTPALCFCQETDNKLYGRTNNPWDISKSAGGSSGG
EGALLAVGGAAVGIGSDVGGSIRFPAHFNGVIGFKPGKDQISMDGHFPSIQHDLQARMLTIGPMGKSVQDMRLLYDILSP
SNIESQKLKDFKLEILPGNSGYPLSIETVDILNQLEYFLEKSFPTKRIMPPYFKDSALIWQEIMSINGSKLIEDEAYNND
RSGVYSSFLKEKLTQRTSVHPYLSWAIIGAKMFKPSHKRVNEILTIIEQGDGVIETYLDNRLLIFPVYHETALPHGKVFK
EIFSIRKTYLQYMPYVAYANVWGLPSLTIPVGESKNGLPISIQIMSKPGNEDAIFRLGRLIEKKYRGFKLAPIPNVD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=29.6747967479675, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI166795287, Length=210, Percent_Identity=36.6666666666667, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI222831590, Length=247, Percent_Identity=25.9109311740891, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=29.4, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17556264, Length=234, Percent_Identity=34.1880341880342, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538252, Length=256, Percent_Identity=34.375, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71990152, Length=233, Percent_Identity=35.1931330472103, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17538254, Length=208, Percent_Identity=34.1346153846154, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=265, Percent_Identity=32.0754716981132, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556278, Length=265, Percent_Identity=32.0754716981132, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=453, Percent_Identity=23.1788079470199, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32565337, Length=509, Percent_Identity=22.3968565815324, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=235, Percent_Identity=31.9148936170213, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319685, Length=142, Percent_Identity=37.3239436619718, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=30.6122448979592, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=30.6122448979592, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=30.6122448979592, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=30.6122448979592, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24648435, Length=495, Percent_Identity=30.3030303030303, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24648437, Length=495, Percent_Identity=30.3030303030303, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45550774, Length=495, Percent_Identity=30.3030303030303, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=27.906976744186, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=28.968253968254, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI21356731, Length=495, Percent_Identity=27.2727272727273, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24648441, Length=429, Percent_Identity=28.9044289044289, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24648439, Length=429, Percent_Identity=28.9044289044289, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24648113, Length=214, Percent_Identity=34.5794392523364, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 52796; Mature: 52796

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDTQIIQMDATQLSKAILSSELTSVKAVAAFIKHIHEVNPIINALVEDRFIEAIEEAKE
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDNLLKNGQKRGPLHGVPISIKESLHVTGLKTTGGLEHRQDLIAIEDAAVVKKLKEAGAI
HHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHEEEEHHHHHHHHHHHCCEE
IIGKTNTPALCFCQETDNKLYGRTNNPWDISKSAGGSSGGEGALLAVGGAAVGIGSDVGG
EEECCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCC
SIRFPAHFNGVIGFKPGKDQISMDGHFPSIQHDLQARMLTIGPMGKSVQDMRLLYDILSP
CEECCCCCCCEEECCCCCCCEEECCCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHCC
SNIESQKLKDFKLEILPGNSGYPLSIETVDILNQLEYFLEKSFPTKRIMPPYFKDSALIW
CCCCHHHHCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
QEIMSINGSKLIEDEAYNNDRSGVYSSFLKEKLTQRTSVHPYLSWAIIGAKMFKPSHKRV
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
NEILTIIEQGDGVIETYLDNRLLIFPVYHETALPHGKVFKEIFSIRKTYLQYMPYVAYAN
HHHHHHHHCCCCEEEHHHCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VWGLPSLTIPVGESKNGLPISIQIMSKPGNEDAIFRLGRLIEKKYRGFKLAPIPNVD
HCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCC
>Mature Secondary Structure
MDDTQIIQMDATQLSKAILSSELTSVKAVAAFIKHIHEVNPIINALVEDRFIEAIEEAKE
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDNLLKNGQKRGPLHGVPISIKESLHVTGLKTTGGLEHRQDLIAIEDAAVVKKLKEAGAI
HHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHEEEEHHHHHHHHHHHCCEE
IIGKTNTPALCFCQETDNKLYGRTNNPWDISKSAGGSSGGEGALLAVGGAAVGIGSDVGG
EEECCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCCCCC
SIRFPAHFNGVIGFKPGKDQISMDGHFPSIQHDLQARMLTIGPMGKSVQDMRLLYDILSP
CEECCCCCCCEEECCCCCCCEEECCCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHCC
SNIESQKLKDFKLEILPGNSGYPLSIETVDILNQLEYFLEKSFPTKRIMPPYFKDSALIW
CCCCHHHHCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
QEIMSINGSKLIEDEAYNNDRSGVYSSFLKEKLTQRTSVHPYLSWAIIGAKMFKPSHKRV
HHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
NEILTIIEQGDGVIETYLDNRLLIFPVYHETALPHGKVFKEIFSIRKTYLQYMPYVAYAN
HHHHHHHHCCCCEEEHHHCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VWGLPSLTIPVGESKNGLPISIQIMSKPGNEDAIFRLGRLIEKKYRGFKLAPIPNVD
HCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]