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The map label for this gene is dapF

Identifier: 23099809

GI number: 23099809

Start: 2406943

End: 2407803

Strand: Reverse

Name: dapF

Synonym: OB2354

Alternate gene names: 23099809

Gene position: 2407803-2406943 (Counterclockwise)

Preceding gene: 23099810

Following gene: 23099808

Centisome position: 66.32

GC content: 36.35

Gene sequence:

>861_bases
ATGCAGATACCTTTTACAAAAATGCATGGATTAGGAAATAATTATGTTTATATTGACTTATTTGATTATGCTATAGATGA
AAAGCTATATCATGATCTGGCCATTCAAATTTCAGATGTTTATACAGGTATTGGCTCGGATGGGATGATTCTAATTCACC
CCACTACGGATGCTGATGTGGGCATGCGTATTTTTAATAAGGATGGATCAGAAGGTAAAAGCTGTGGGAATGGATTACGT
TGTACAGCAAAATACGCATATGAAAATGGAATTGTTACGAATAAGAAGTTCCATATTCAAACGAAAGCAAATATTGTTGA
AGCCGAAGTGTCTGTAGATAATAATAAAGTAAATCAAGTAACAATCGATATGGGAGAACCTATTTTAGCAAGAAATGAAA
TTCCGATGATGGGAAATAATGATGTAAAAGTTGTAAATGAACCATTTGTTGTAGCTGGAGAAACACAATATATTACTGCA
GTCTCAATGGGAAATCCCCATGGTGTATTCTTTGTTGATGATATTGAAAAGGCTCCATTAGAAACACTCGGACCTACTAT
TGAAAAGGATATGCGTTTTCCGGAAAGTATTAATGTGGAATTTATCGAAGTTGTCTCGCCAACCGAACTCAATTTTCGTG
TATGGGAGCGTGGATCTGGAATAACTCAGGCTTGTGGAACAGGTGCGTGTGCTGCTGTAGTTGCCGCAACTTTAAATGAA
AAAGTAACGAAGTCACACCCAATTGTTGTTCATCTTGCTGGAGGAGATTTAAATATTGAATGGAAAGAAGATAACCATGT
TTGGATGACTGGCCCAGCAGAGGTAATTGCAACTGGACTATTTTATTATAAAGAGGAATAA

Upstream 100 bases:

>100_bases
GCAGAAGGTATCCCGACATTAAAGCCTATATATCGAACTTTAGATGATCTAGGATTACCACTAGTTAATTAACTTTTCAA
AAGAAAGCAGGAGATTAGGG

Downstream 100 bases:

>100_bases
CTATTTCAAACGCTTGAAACTCTGTAGAACATGTGTATACTAAAATTATGGAACAATATAAGGAGGTTCGTCATGATTAT
AAATATAACTGAAAGTGCAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MQIPFTKMHGLGNNYVYIDLFDYAIDEKLYHDLAIQISDVYTGIGSDGMILIHPTTDADVGMRIFNKDGSEGKSCGNGLR
CTAKYAYENGIVTNKKFHIQTKANIVEAEVSVDNNKVNQVTIDMGEPILARNEIPMMGNNDVKVVNEPFVVAGETQYITA
VSMGNPHGVFFVDDIEKAPLETLGPTIEKDMRFPESINVEFIEVVSPTELNFRVWERGSGITQACGTGACAAVVAATLNE
KVTKSHPIVVHLAGGDLNIEWKEDNHVWMTGPAEVIATGLFYYKEE

Sequences:

>Translated_286_residues
MQIPFTKMHGLGNNYVYIDLFDYAIDEKLYHDLAIQISDVYTGIGSDGMILIHPTTDADVGMRIFNKDGSEGKSCGNGLR
CTAKYAYENGIVTNKKFHIQTKANIVEAEVSVDNNKVNQVTIDMGEPILARNEIPMMGNNDVKVVNEPFVVAGETQYITA
VSMGNPHGVFFVDDIEKAPLETLGPTIEKDMRFPESINVEFIEVVSPTELNFRVWERGSGITQACGTGACAAVVAATLNE
KVTKSHPIVVHLAGGDLNIEWKEDNHVWMTGPAEVIATGLFYYKEE
>Mature_286_residues
MQIPFTKMHGLGNNYVYIDLFDYAIDEKLYHDLAIQISDVYTGIGSDGMILIHPTTDADVGMRIFNKDGSEGKSCGNGLR
CTAKYAYENGIVTNKKFHIQTKANIVEAEVSVDNNKVNQVTIDMGEPILARNEIPMMGNNDVKVVNEPFVVAGETQYITA
VSMGNPHGVFFVDDIEKAPLETLGPTIEKDMRFPESINVEFIEVVSPTELNFRVWERGSGITQACGTGACAAVVAATLNE
KVTKSHPIVVHLAGGDLNIEWKEDNHVWMTGPAEVIATGLFYYKEE

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=285, Percent_Identity=36.8421052631579, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_OCEIH (Q8ENX2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693275.1
- ProteinModelPortal:   Q8ENX2
- SMR:   Q8ENX2
- GeneID:   1017278
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2354
- NMPDR:   fig|221109.1.peg.2350
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   OIHE221109:OB2354-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31495; Mature: 31495

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPFTKMHGLGNNYVYIDLFDYAIDEKLYHDLAIQISDVYTGIGSDGMILIHPTTDADV
CCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHHEEEEEEEEEECCCCCCEEEEECCCCCCC
GMRIFNKDGSEGKSCGNGLRCTAKYAYENGIVTNKKFHIQTKANIVEAEVSVDNNKVNQV
CEEEEECCCCCCCCCCCCCEEEEEEEECCCEEECCEEEEEECCEEEEEEEEECCCCEEEE
TIDMGEPILARNEIPMMGNNDVKVVNEPFVVAGETQYITAVSMGNPHGVFFVDDIEKAPL
EEECCCCEEECCCCCCCCCCCEEEEECCEEEECCCEEEEEEECCCCCEEEEEECCCCCCH
ETLGPTIEKDMRFPESINVEFIEVVSPTELNFRVWERGSGITQACGTGACAAVVAATLNE
HHHCCCHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHH
KVTKSHPIVVHLAGGDLNIEWKEDNHVWMTGPAEVIATGLFYYKEE
HHCCCCCEEEEEECCCEEEEEECCCEEEEECCHHHHEEEEEEEECC
>Mature Secondary Structure
MQIPFTKMHGLGNNYVYIDLFDYAIDEKLYHDLAIQISDVYTGIGSDGMILIHPTTDADV
CCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHHEEEEEEEEEECCCCCCEEEEECCCCCCC
GMRIFNKDGSEGKSCGNGLRCTAKYAYENGIVTNKKFHIQTKANIVEAEVSVDNNKVNQV
CEEEEECCCCCCCCCCCCCEEEEEEEECCCEEECCEEEEEECCEEEEEEEEECCCCEEEE
TIDMGEPILARNEIPMMGNNDVKVVNEPFVVAGETQYITAVSMGNPHGVFFVDDIEKAPL
EEECCCCEEECCCCCCCCCCCEEEEECCEEEECCCEEEEEEECCCCCEEEEEECCCCCCH
ETLGPTIEKDMRFPESINVEFIEVVSPTELNFRVWERGSGITQACGTGACAAVVAATLNE
HHHCCCHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHH
KVTKSHPIVVHLAGGDLNIEWKEDNHVWMTGPAEVIATGLFYYKEE
HHCCCCCEEEEEECCCEEEEEECCCEEEEECCHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376