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The map label for this gene is pckA

Identifier: 23099770

GI number: 23099770

Start: 2365719

End: 2367302

Strand: Direct

Name: pckA

Synonym: OB2315

Alternate gene names: 23099770

Gene position: 2365719-2367302 (Clockwise)

Preceding gene: 23099767

Following gene: 23099771

Centisome position: 65.16

GC content: 35.92

Gene sequence:

>1584_bases
ATGAAAACTGTTGATAAGTCTTATATGAAAGAATTACAAACAAATCATATTCACCACAATCTATCGGTAGCACAGCTCGT
TGAAAAAATTCTGTATAGACAAGAAGGAATTCTTACATCTACCGGAGCAGTACGCGCCACTACAGGAACTTATACTGGTC
GATCTCCGGAAGATAAATTTACCGTTAAAGATGAAGTTTCGGATCAACATGTTAACTGGGGTAAAGTTAATAAACCCATC
CAGGAGGAAGTATTCAACCAATTATTATCTAAAGTGATTTCTTATTTAAATGAAAAACAAGAAATATTTAAATTTCAAGG
ATTTGCGGGGTCTGATCATACTTACCGACTCCCTATTCAAGTGATTAATGAATATGCTTGGCATAATTTATTTTCTAGAC
AACTATTCATTACTCCAACGGAAGAGGAATTAAACAATCACCAAGCAGAATTTACAGTTATTTCAGCTCCAGGATTTAAG
GCTGATCCAGCTATTGATGGAACAAATTCAGAAACATTTATATTAATTTCTTTTAAGAAAAGAATTGTACTTATCGGTGG
TACTGAATATGCCGGAGAAATTAAAAAGTCCATTTTTTCTGTGATGAATTATTTATTACCACAGCAAGATGTGCTTTCTA
TGCATTGCTCCGCTAATGTAGGCCAGGAAGGTGACGTTGCATTATTTTTCGGGTTATCAGGTACCGGGAAAACGACATTA
TCCGCAGACCCCTACCGAAAATTAATCGGTGATGACGAACATGGTTGGAGTCCAAATGGTGTATTCAATATTGAAGGCGG
TTGTTATGCGAAATGTATCAATCTCTCAGAAGAAAAGGAACCACAAATTTATAACGCAATCCGTTACGGTACTGTGTTAG
AGAATGTGATTTTAAATGACAATAGCAGAGAACCCGATTATGATGATACTTCATTAACAGAAAACACACGTGCTGCTTAT
CCTTTAGAGAATATAGACAATATTATTAATCCTAGTGTTGCAGGGCATCCAAATACAATTATTTTCTTAACTGCTGACGC
ATCAGGCACATTGCCTCCGATTAGCAAACTGACAAAAGAACAAGCGATGTATCATTTTCTAAGCGGATATACAAGTAAAT
TAGCAGGAACAGAACGAGGAGTAACAGAACCACTAGCTACTTTCTCTGCTTGTTTTGGTTCTCCATTCTTACCATTAGCT
CCATCTAAATATGCAGAAATGCTTGGAAAGAAAATTGATATGTTTGATACAAATGTATTTTTGATTAATACCGGATGGAC
TGGCGGATCATATGGAGTGGGTGACCGTATTAAACTTTCCTTCACCAGAGCAATGGTTCACTCTGCATTAGAAGGAGAAT
TAAATTCTATAGAAACAATCACTGATGAAATTTTCGGTTTACAGATTCCTGCTCATGTTCCTGGAGTCCCTGATGAATTA
TTAGTACCTAAACAGACATGGGGTAATAAAGAAGCATATATGAAAGAAGCACAAACACTTGCATTAAAATTCCACGAAAA
CTTTAAGAAATTCACTCTAGCTAGTGAAACGATTAAGCAAGCTGGTCCTTTATATAAAGGATAA

Upstream 100 bases:

>100_bases
GGACTATTTATTTTAATGTGTTATACTAATAATCAGATTTAATAAATATGGATCGTTAAGAGATTAAAACTACTCTAATT
AATAGTAAGGAGATTATACC

Downstream 100 bases:

>100_bases
TTTAAACATGTTTACTCATCCCATTAAGGATTAATATAAATGAGTGAAGTACTCGTTCGATATTTGCGAACAACCAGTCT
CAATACGAGGCTGGTTTTTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MKTVDKSYMKELQTNHIHHNLSVAQLVEKILYRQEGILTSTGAVRATTGTYTGRSPEDKFTVKDEVSDQHVNWGKVNKPI
QEEVFNQLLSKVISYLNEKQEIFKFQGFAGSDHTYRLPIQVINEYAWHNLFSRQLFITPTEEELNNHQAEFTVISAPGFK
ADPAIDGTNSETFILISFKKRIVLIGGTEYAGEIKKSIFSVMNYLLPQQDVLSMHCSANVGQEGDVALFFGLSGTGKTTL
SADPYRKLIGDDEHGWSPNGVFNIEGGCYAKCINLSEEKEPQIYNAIRYGTVLENVILNDNSREPDYDDTSLTENTRAAY
PLENIDNIINPSVAGHPNTIIFLTADASGTLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTEPLATFSACFGSPFLPLA
PSKYAEMLGKKIDMFDTNVFLINTGWTGGSYGVGDRIKLSFTRAMVHSALEGELNSIETITDEIFGLQIPAHVPGVPDEL
LVPKQTWGNKEAYMKEAQTLALKFHENFKKFTLASETIKQAGPLYKG

Sequences:

>Translated_527_residues
MKTVDKSYMKELQTNHIHHNLSVAQLVEKILYRQEGILTSTGAVRATTGTYTGRSPEDKFTVKDEVSDQHVNWGKVNKPI
QEEVFNQLLSKVISYLNEKQEIFKFQGFAGSDHTYRLPIQVINEYAWHNLFSRQLFITPTEEELNNHQAEFTVISAPGFK
ADPAIDGTNSETFILISFKKRIVLIGGTEYAGEIKKSIFSVMNYLLPQQDVLSMHCSANVGQEGDVALFFGLSGTGKTTL
SADPYRKLIGDDEHGWSPNGVFNIEGGCYAKCINLSEEKEPQIYNAIRYGTVLENVILNDNSREPDYDDTSLTENTRAAY
PLENIDNIINPSVAGHPNTIIFLTADASGTLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTEPLATFSACFGSPFLPLA
PSKYAEMLGKKIDMFDTNVFLINTGWTGGSYGVGDRIKLSFTRAMVHSALEGELNSIETITDEIFGLQIPAHVPGVPDEL
LVPKQTWGNKEAYMKEAQTLALKFHENFKKFTLASETIKQAGPLYKG
>Mature_527_residues
MKTVDKSYMKELQTNHIHHNLSVAQLVEKILYRQEGILTSTGAVRATTGTYTGRSPEDKFTVKDEVSDQHVNWGKVNKPI
QEEVFNQLLSKVISYLNEKQEIFKFQGFAGSDHTYRLPIQVINEYAWHNLFSRQLFITPTEEELNNHQAEFTVISAPGFK
ADPAIDGTNSETFILISFKKRIVLIGGTEYAGEIKKSIFSVMNYLLPQQDVLSMHCSANVGQEGDVALFFGLSGTGKTTL
SADPYRKLIGDDEHGWSPNGVFNIEGGCYAKCINLSEEKEPQIYNAIRYGTVLENVILNDNSREPDYDDTSLTENTRAAY
PLENIDNIINPSVAGHPNTIIFLTADASGTLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTEPLATFSACFGSPFLPLA
PSKYAEMLGKKIDMFDTNVFLINTGWTGGSYGVGDRIKLSFTRAMVHSALEGELNSIETITDEIFGLQIPAHVPGVPDEL
LVPKQTWGNKEAYMKEAQTLALKFHENFKKFTLASETIKQAGPLYKG

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=504, Percent_Identity=48.8095238095238, Blast_Score=481, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6322950, Length=517, Percent_Identity=51.063829787234, Blast_Score=494, Evalue=1e-140,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_OCEIH (Q8EP04)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693236.1
- ProteinModelPortal:   Q8EP04
- SMR:   Q8EP04
- GeneID:   1016661
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2315
- NMPDR:   fig|221109.1.peg.2312
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- BioCyc:   OIHE221109:OB2315-MONOMER
- BRENDA:   4.1.1.49
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58559; Mature: 58559

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVDKSYMKELQTNHIHHNLSVAQLVEKILYRQEGILTSTGAVRATTGTYTGRSPEDKF
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCCE
TVKDEVSDQHVNWGKVNKPIQEEVFNQLLSKVISYLNEKQEIFKFQGFAGSDHTYRLPIQ
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEECHHH
VINEYAWHNLFSRQLFITPTEEELNNHQAEFTVISAPGFKADPAIDGTNSETFILISFKK
HHHHHHHHHHHCCCEEECCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECC
RIVLIGGTEYAGEIKKSIFSVMNYLLPQQDVLSMHCSANVGQEGDVALFFGLSGTGKTTL
EEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
SADPYRKLIGDDEHGWSPNGVFNIEGGCYAKCINLSEEKEPQIYNAIRYGTVLENVILND
CCCHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
NSREPDYDDTSLTENTRAAYPLENIDNIINPSVAGHPNTIIFLTADASGTLPPISKLTKE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
QAMYHFLSGYTSKLAGTERGVTEPLATFSACFGSPFLPLAPSKYAEMLGKKIDMFDTNVF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEECCCEE
LINTGWTGGSYGVGDRIKLSFTRAMVHSALEGELNSIETITDEIFGLQIPAHVPGVPDEL
EEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCCHHH
LVPKQTWGNKEAYMKEAQTLALKFHENFKKFTLASETIKQAGPLYKG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTVDKSYMKELQTNHIHHNLSVAQLVEKILYRQEGILTSTGAVRATTGTYTGRSPEDKF
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCCE
TVKDEVSDQHVNWGKVNKPIQEEVFNQLLSKVISYLNEKQEIFKFQGFAGSDHTYRLPIQ
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEECHHH
VINEYAWHNLFSRQLFITPTEEELNNHQAEFTVISAPGFKADPAIDGTNSETFILISFKK
HHHHHHHHHHHCCCEEECCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECC
RIVLIGGTEYAGEIKKSIFSVMNYLLPQQDVLSMHCSANVGQEGDVALFFGLSGTGKTTL
EEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
SADPYRKLIGDDEHGWSPNGVFNIEGGCYAKCINLSEEKEPQIYNAIRYGTVLENVILND
CCCHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
NSREPDYDDTSLTENTRAAYPLENIDNIINPSVAGHPNTIIFLTADASGTLPPISKLTKE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
QAMYHFLSGYTSKLAGTERGVTEPLATFSACFGSPFLPLAPSKYAEMLGKKIDMFDTNVF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEECCCEE
LINTGWTGGSYGVGDRIKLSFTRAMVHSALEGELNSIETITDEIFGLQIPAHVPGVPDEL
EEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCCHHH
LVPKQTWGNKEAYMKEAQTLALKFHENFKKFTLASETIKQAGPLYKG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376