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The map label for this gene is leuS

Identifier: 23099762

GI number: 23099762

Start: 2357022

End: 2359436

Strand: Reverse

Name: leuS

Synonym: OB2307

Alternate gene names: 23099762

Gene position: 2359436-2357022 (Counterclockwise)

Preceding gene: 23099764

Following gene: 23099760

Centisome position: 64.99

GC content: 37.47

Gene sequence:

>2415_bases
ATGAGTTTTCAGCATCGTCAAATAGAAAAGAAATGGCAAGATTATTGGGAAGACAATAAAACTTTTAAAACAGATACGTT
CTCTAATAAGGAAAAGTTCTATGCATTAGATATGTTTCCTTATCCATCAGGGCAGGGATTACACGTAGGGCATCCAGAGG
GATATACCGCTACAGATATTTTAGCCCGTATGAAACGTATGCAAGGATATGAAGTAATGCACCCAATGGGTTGGGATGCG
TTTGGTCTTCCTGCTGAGCAATATGCAATTGATACTGGTAATAGCCCTGCAGAATTTACAAATCAAAATATTAATACGTT
TAAACGTCAGATTAAAGAATTAGGTTTCTCTTATGATTGGGATAGAGAAATAAATACAACTGATCCAAATTACTATAAAT
GGACACAATGGATCTTTAAAAAATTATATGAAAATGACCTAGCATATATGGATGAAGTCGCGGTGAACTGGTGTCCGGCT
TTAGGCACAGTGCTTGCGAATGAAGAAGTAATTGATGGTAAAAGCGAAAGAGGCGGACATCCTGTAATCCGTAAGCCAAT
GAAACAATGGATGTTAAAAATTACGGCATACGCTGACCGTTTGTTAGAAGATTTAAATGAATTAGATTGGCCAGATAGCC
TAAAAGAAATGCAACGAAATTGGATTGGACGCTCAGAAGGTGCGGAAGTTACTTTCTCTATAAAAGGAAACGAAGGGACG
TTTACTGTATTTACTACACGTCCGGATACATTATTTGGAGCAACATATGCTGTTGTCGCTCCGGAACATCCTTTAGTAAA
AGAAATAACAACGTTGAGTCAAAAAGAAGCAGTTCAAAACTATTTAGATGAAATACAGACCAAATCAGATTTGGAAAGAA
CAGATTTAGCTAAAGATAAGACTGGTGTATTTACGGGAGCGTATGCAATTAATCCGGTTAATGGAGAAGAAATGCCAATC
TGGGTTGCGGATTATGTATTAATGAGCTATGGAACGGGAGCAATTATGGCTGTTCCTGCGCATGATGAACGTGATTATGA
ATTTGCAAATACATTTGGCTTACCTATTAAAGAAGTAGTCGCTGGTGGGAATATAGATGATGAGGCTTATACTGGTGATG
GTGAACATGTTAATTCTGAATTCTTGAATGGGTTGGGTAAGGAAGAAGCAATAACGAAAATGATTGATTGGCTAGAAGCT
AATGGTAGTGGGGAGAAAAAAATAACTTATCGTCTACGTGACTGGTTATTTGCTCGTCAACGCTATTGGGGAGAACCAAT
TCCAATTATTCATTGGGAAGACGGTACAATGACAGCTGTACCTGATGAGGATTTACCATTGACACTTCCTCAAGTTGCTG
AAATAAAACCTTCAGGTACGGGAGAATCGCCATTAGCAAATAATACTGATTGGGTAAATGTAGTTGACCCAGAGACTGGA
TTGAAAGGACGTCGTGAAACCAATACAATGCCACAGTGGGCCGGTAGTTGTTGGTATTTCTTACGTTATATTGATCCAAA
TAATACAGAGCGATTGGCAGATCCAAAGGCATTAGAAGAATGGCTACCTATCGATATATATATAGGTGGAGCAGAACATG
CGGTTCTCCATCTGTTATATGCTCGTTTCTGGCATAAATTCTTATTTGATATTGGAGTCGTCTCCACAAAAGAACCATTC
CAAAAGCTATATAATCAAGGCATGATTCTTGGTGAAGGCAACGAAAAAATGAGTAAATCCAAAGGGAACGTAGTGAATCC
TGATGATATAATCGATTCGCATGGCGCAGACACATTACGTTTATACGAGATGTTTATGGGACCGCTAGATGCTTCTGTTG
CTTGGTCTACAAACGGATTAGATGGATCGCGTAGATTTTTAGATCGTGTATGGAGATTGTATGTAGGTGATGATGGTTCG
CTTACAGATAAAATTATTGATGATGAATCCACTGAATTAGACAAGATTTATCATGAAACAGTGAAAAAAGTGACAGATGA
CTTTGAACATATGCATTTTAATACTGGTATTTCACAAATGATGGTATTTATTAATGAATGCTATAAAGCGAATAAAATTC
CTAAAATTTATGCGGAAGGCTTTGTTAAATTACTGTCACCAGTAGCACCACATTTAAGTGAAGAAATTTGGCAAAAACTT
GGACACTCAGAATCAATAAGTAAGGCTACTTGGCCAGCATATGATGAATCAAAATTAGTAGAAGATGAAGTGGAAGTCGT
TCTACAGATCATGGGTAAGGTTCGATCTAAAATTCAGGTTCCTGTTGATATTAGTAAGGATGATCTTGAAAAAGCGGCAT
TAGATGACGAAAGCATGCAAAAATGGTTAGAAGGAAAAACTATCCGTAAAGTTATCGTTGTGCCAGGAAAGCTTGTAAAT
ATAGTAGCAAATTAA

Upstream 100 bases:

>100_bases
TAAAAGCTTCTCGTCCCTGTTTATGAGGGATGAGTGGCTTTTTTTAGTTTACTCAAAAAATTAAAGTGTAAGCAGTTTTA
CTATGAAAGGAAGGGTTGAC

Downstream 100 bases:

>100_bases
AAAAAGAAGCATTGATCTGGAAATGGTCAATGCTTCTTTTTATAGAGGTTATTCATCTTTATTTTCCTCTGTTGGATTTT
CCGTTGGATTATCTGATTTC

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MSFQHRQIEKKWQDYWEDNKTFKTDTFSNKEKFYALDMFPYPSGQGLHVGHPEGYTATDILARMKRMQGYEVMHPMGWDA
FGLPAEQYAIDTGNSPAEFTNQNINTFKRQIKELGFSYDWDREINTTDPNYYKWTQWIFKKLYENDLAYMDEVAVNWCPA
LGTVLANEEVIDGKSERGGHPVIRKPMKQWMLKITAYADRLLEDLNELDWPDSLKEMQRNWIGRSEGAEVTFSIKGNEGT
FTVFTTRPDTLFGATYAVVAPEHPLVKEITTLSQKEAVQNYLDEIQTKSDLERTDLAKDKTGVFTGAYAINPVNGEEMPI
WVADYVLMSYGTGAIMAVPAHDERDYEFANTFGLPIKEVVAGGNIDDEAYTGDGEHVNSEFLNGLGKEEAITKMIDWLEA
NGSGEKKITYRLRDWLFARQRYWGEPIPIIHWEDGTMTAVPDEDLPLTLPQVAEIKPSGTGESPLANNTDWVNVVDPETG
LKGRRETNTMPQWAGSCWYFLRYIDPNNTERLADPKALEEWLPIDIYIGGAEHAVLHLLYARFWHKFLFDIGVVSTKEPF
QKLYNQGMILGEGNEKMSKSKGNVVNPDDIIDSHGADTLRLYEMFMGPLDASVAWSTNGLDGSRRFLDRVWRLYVGDDGS
LTDKIIDDESTELDKIYHETVKKVTDDFEHMHFNTGISQMMVFINECYKANKIPKIYAEGFVKLLSPVAPHLSEEIWQKL
GHSESISKATWPAYDESKLVEDEVEVVLQIMGKVRSKIQVPVDISKDDLEKAALDDESMQKWLEGKTIRKVIVVPGKLVN
IVAN

Sequences:

>Translated_804_residues
MSFQHRQIEKKWQDYWEDNKTFKTDTFSNKEKFYALDMFPYPSGQGLHVGHPEGYTATDILARMKRMQGYEVMHPMGWDA
FGLPAEQYAIDTGNSPAEFTNQNINTFKRQIKELGFSYDWDREINTTDPNYYKWTQWIFKKLYENDLAYMDEVAVNWCPA
LGTVLANEEVIDGKSERGGHPVIRKPMKQWMLKITAYADRLLEDLNELDWPDSLKEMQRNWIGRSEGAEVTFSIKGNEGT
FTVFTTRPDTLFGATYAVVAPEHPLVKEITTLSQKEAVQNYLDEIQTKSDLERTDLAKDKTGVFTGAYAINPVNGEEMPI
WVADYVLMSYGTGAIMAVPAHDERDYEFANTFGLPIKEVVAGGNIDDEAYTGDGEHVNSEFLNGLGKEEAITKMIDWLEA
NGSGEKKITYRLRDWLFARQRYWGEPIPIIHWEDGTMTAVPDEDLPLTLPQVAEIKPSGTGESPLANNTDWVNVVDPETG
LKGRRETNTMPQWAGSCWYFLRYIDPNNTERLADPKALEEWLPIDIYIGGAEHAVLHLLYARFWHKFLFDIGVVSTKEPF
QKLYNQGMILGEGNEKMSKSKGNVVNPDDIIDSHGADTLRLYEMFMGPLDASVAWSTNGLDGSRRFLDRVWRLYVGDDGS
LTDKIIDDESTELDKIYHETVKKVTDDFEHMHFNTGISQMMVFINECYKANKIPKIYAEGFVKLLSPVAPHLSEEIWQKL
GHSESISKATWPAYDESKLVEDEVEVVLQIMGKVRSKIQVPVDISKDDLEKAALDDESMQKWLEGKTIRKVIVVPGKLVN
IVAN
>Mature_803_residues
SFQHRQIEKKWQDYWEDNKTFKTDTFSNKEKFYALDMFPYPSGQGLHVGHPEGYTATDILARMKRMQGYEVMHPMGWDAF
GLPAEQYAIDTGNSPAEFTNQNINTFKRQIKELGFSYDWDREINTTDPNYYKWTQWIFKKLYENDLAYMDEVAVNWCPAL
GTVLANEEVIDGKSERGGHPVIRKPMKQWMLKITAYADRLLEDLNELDWPDSLKEMQRNWIGRSEGAEVTFSIKGNEGTF
TVFTTRPDTLFGATYAVVAPEHPLVKEITTLSQKEAVQNYLDEIQTKSDLERTDLAKDKTGVFTGAYAINPVNGEEMPIW
VADYVLMSYGTGAIMAVPAHDERDYEFANTFGLPIKEVVAGGNIDDEAYTGDGEHVNSEFLNGLGKEEAITKMIDWLEAN
GSGEKKITYRLRDWLFARQRYWGEPIPIIHWEDGTMTAVPDEDLPLTLPQVAEIKPSGTGESPLANNTDWVNVVDPETGL
KGRRETNTMPQWAGSCWYFLRYIDPNNTERLADPKALEEWLPIDIYIGGAEHAVLHLLYARFWHKFLFDIGVVSTKEPFQ
KLYNQGMILGEGNEKMSKSKGNVVNPDDIIDSHGADTLRLYEMFMGPLDASVAWSTNGLDGSRRFLDRVWRLYVGDDGSL
TDKIIDDESTELDKIYHETVKKVTDDFEHMHFNTGISQMMVFINECYKANKIPKIYAEGFVKLLSPVAPHLSEEIWQKLG
HSESISKATWPAYDESKLVEDEVEVVLQIMGKVRSKIQVPVDISKDDLEKAALDDESMQKWLEGKTIRKVIVVPGKLVNI
VAN

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=872, Percent_Identity=33.0275229357798, Blast_Score=470, Evalue=1e-132,
Organism=Escherichia coli, GI1786861, Length=866, Percent_Identity=42.6096997690531, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=391, Percent_Identity=24.8081841432225, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71997510, Length=813, Percent_Identity=29.2742927429274, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI71997517, Length=806, Percent_Identity=29.4044665012407, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI212645227, Length=335, Percent_Identity=28.955223880597, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17510661, Length=936, Percent_Identity=20.5128205128205, Blast_Score=118, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323414, Length=875, Percent_Identity=35.4285714285714, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321531, Length=369, Percent_Identity=23.3062330623306, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355409, Length=873, Percent_Identity=30.6987399770905, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_OCEIH (Q8EP12)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693228.1
- ProteinModelPortal:   Q8EP12
- SMR:   Q8EP12
- GeneID:   1015873
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2307
- NMPDR:   fig|221109.1.peg.2304
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- ProtClustDB:   PRK00390
- BioCyc:   OIHE221109:OB2307-MONOMER
- BRENDA:   6.1.1.4
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 91702; Mature: 91571

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 579-579

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQHRQIEKKWQDYWEDNKTFKTDTFSNKEKFYALDMFPYPSGQGLHVGHPEGYTATDI
CCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LARMKRMQGYEVMHPMGWDAFGLPAEQYAIDTGNSPAEFTNQNINTFKRQIKELGFSYDW
HHHHHHHCCCEEECCCCCCCCCCCHHHEEECCCCCCHHHCCCCHHHHHHHHHHCCCCCCC
DREINTTDPNYYKWTQWIFKKLYENDLAYMDEVAVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCC
PVIRKPMKQWMLKITAYADRLLEDLNELDWPDSLKEMQRNWIGRSEGAEVTFSIKGNEGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCE
FTVFTTRPDTLFGATYAVVAPEHPLVKEITTLSQKEAVQNYLDEIQTKSDLERTDLAKDK
EEEEECCCCCHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
TGVFTGAYAINPVNGEEMPIWVADYVLMSYGTGAIMAVPAHDERDYEFANTFGLPIKEVV
CCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCHHHHH
AGGNIDDEAYTGDGEHVNSEFLNGLGKEEAITKMIDWLEANGSGEKKITYRLRDWLFARQ
CCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
RYWGEPIPIIHWEDGTMTAVPDEDLPLTLPQVAEIKPSGTGESPLANNTDWVNVVDPETG
HHCCCCCCEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
LKGRRETNTMPQWAGSCWYFLRYIDPNNTERLADPKALEEWLPIDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHH
ARFWHKFLFDIGVVSTKEPFQKLYNQGMILGEGNEKMSKSKGNVVNPDDIIDSHGADTLR
HHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHCCCCCHHHH
LYEMFMGPLDASVAWSTNGLDGSRRFLDRVWRLYVGDDGSLTDKIIDDESTELDKIYHET
HHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHEEECCCCCCCHHHCCCCCHHHHHHHHHH
VKKVTDDFEHMHFNTGISQMMVFINECYKANKIPKIYAEGFVKLLSPVAPHLSEEIWQKL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GHSESISKATWPAYDESKLVEDEVEVVLQIMGKVRSKIQVPVDISKDDLEKAALDDESMQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCHHHHH
KWLEGKTIRKVIVVPGKLVNIVAN
HHHCCCCEEEEEECCCHHHHEECC
>Mature Secondary Structure 
SFQHRQIEKKWQDYWEDNKTFKTDTFSNKEKFYALDMFPYPSGQGLHVGHPEGYTATDI
CCCHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCCCCCEECCCCCCCCHHHH
LARMKRMQGYEVMHPMGWDAFGLPAEQYAIDTGNSPAEFTNQNINTFKRQIKELGFSYDW
HHHHHHHCCCEEECCCCCCCCCCCHHHEEECCCCCCHHHCCCCHHHHHHHHHHCCCCCCC
DREINTTDPNYYKWTQWIFKKLYENDLAYMDEVAVNWCPALGTVLANEEVIDGKSERGGH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCC
PVIRKPMKQWMLKITAYADRLLEDLNELDWPDSLKEMQRNWIGRSEGAEVTFSIKGNEGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCE
FTVFTTRPDTLFGATYAVVAPEHPLVKEITTLSQKEAVQNYLDEIQTKSDLERTDLAKDK
EEEEECCCCCHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
TGVFTGAYAINPVNGEEMPIWVADYVLMSYGTGAIMAVPAHDERDYEFANTFGLPIKEVV
CCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCHHHHH
AGGNIDDEAYTGDGEHVNSEFLNGLGKEEAITKMIDWLEANGSGEKKITYRLRDWLFARQ
CCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
RYWGEPIPIIHWEDGTMTAVPDEDLPLTLPQVAEIKPSGTGESPLANNTDWVNVVDPETG
HHCCCCCCEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
LKGRRETNTMPQWAGSCWYFLRYIDPNNTERLADPKALEEWLPIDIYIGGAEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHH
ARFWHKFLFDIGVVSTKEPFQKLYNQGMILGEGNEKMSKSKGNVVNPDDIIDSHGADTLR
HHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHCCCCCHHHH
LYEMFMGPLDASVAWSTNGLDGSRRFLDRVWRLYVGDDGSLTDKIIDDESTELDKIYHET
HHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHEEECCCCCCCHHHCCCCCHHHHHHHHHH
VKKVTDDFEHMHFNTGISQMMVFINECYKANKIPKIYAEGFVKLLSPVAPHLSEEIWQKL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GHSESISKATWPAYDESKLVEDEVEVVLQIMGKVRSKIQVPVDISKDDLEKAALDDESMQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCHHHHH
KWLEGKTIRKVIVVPGKLVNIVAN
HHHCCCCEEEEEECCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376