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The map label for this gene is rimK [C]

Identifier: 23099514

GI number: 23099514

Start: 2084856

End: 2085746

Strand: Direct

Name: rimK [C]

Synonym: OB2059

Alternate gene names: 23099514

Gene position: 2084856-2085746 (Clockwise)

Preceding gene: 23099486

Following gene: 23099515

Centisome position: 57.43

GC content: 33.56

Gene sequence:

>891_bases
ATGAAAAAGACTGGGTGGATTATTGTCAATGGCAATCTAAAAACTGATAAATTCATCGACTATGCAAAAATGATACAAGC
TGCTGCAATAAAAAGACATATCGATATCAGTATTATGAAAAACAACGATATTTTACTTGATTTATCTGATAAGCAACCGC
TACATTTACATAAACAAACATATACTTTACCTGATTTTGCATTCTTTATTGATAAAGATATCTATTTGGCACAATATCTG
GAACAACTTGGAATACCTGTCTTCAACAGTTCTTCTACGATTGAGATGAGTGATGATAAGATTCAGACGTATCAAAGACT
CACCTCTCTAGATATACCTATTCCACATACCATAATTGCTCCCAAAATCTATACCAAAGGTGTACTTTCTGATGATTATC
TACAATCCATTATTCATAAACTAGGTATACCATTAGTTGTTAAAGAAGCGTTTGGTTCTTTTGGTGAACAAGTTTACCTC
GTGAATAATGTTGAGGAACTTAAAGAATTGACGAATAATTTACAAGGAAAGCCATTTTTATTTCAATCTTATATTTCCTC
TAGCCATGGTAGAGATATTCGATTACAAGTGGTAGGGAATGAAGTCATTGCAGGTATGGAACGGAATAATAATCAAGATT
TTAGAGCCAATATAACCAATGGTGGGGATATGAAACCATTTACGCCTGATCAAAAAGCTATAAATATTGCTGTTGATGCC
GCACAAGCCATTAATGCTGATTTTGCTGGAGTGGACTTATTATTTGGTCCGGAGAACTCCTATTTTGTATGTGAAATTAA
CGCTAATGCCCATATTCGTAATTTGACAGAATGTACGGGTGTGAATATAGCGGATAAAATGATAACCTATATCGAAAGGA
AAATAAGATGA

Upstream 100 bases:

>100_bases
TCTCTATTATTAAAATATTGTTCTATTTGTTCAAATAACATACTTTCCCTACTTTCCATTCCATCATATAATAATTATAG
CAGAAAGGATGATTCGTTTA

Downstream 100 bases:

>100_bases
CTTCAGGTTGGTTAATATACTCAGAAAAAGACGCAATAAAAAATGCAAGCTATATTGAATGGTTTCAATTAGAGGCAACA
AAACAAGGCCTTTCCTTACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKKTGWIIVNGNLKTDKFIDYAKMIQAAAIKRHIDISIMKNNDILLDLSDKQPLHLHKQTYTLPDFAFFIDKDIYLAQYL
EQLGIPVFNSSSTIEMSDDKIQTYQRLTSLDIPIPHTIIAPKIYTKGVLSDDYLQSIIHKLGIPLVVKEAFGSFGEQVYL
VNNVEELKELTNNLQGKPFLFQSYISSSHGRDIRLQVVGNEVIAGMERNNNQDFRANITNGGDMKPFTPDQKAINIAVDA
AQAINADFAGVDLLFGPENSYFVCEINANAHIRNLTECTGVNIADKMITYIERKIR

Sequences:

>Translated_296_residues
MKKTGWIIVNGNLKTDKFIDYAKMIQAAAIKRHIDISIMKNNDILLDLSDKQPLHLHKQTYTLPDFAFFIDKDIYLAQYL
EQLGIPVFNSSSTIEMSDDKIQTYQRLTSLDIPIPHTIIAPKIYTKGVLSDDYLQSIIHKLGIPLVVKEAFGSFGEQVYL
VNNVEELKELTNNLQGKPFLFQSYISSSHGRDIRLQVVGNEVIAGMERNNNQDFRANITNGGDMKPFTPDQKAINIAVDA
AQAINADFAGVDLLFGPENSYFVCEINANAHIRNLTECTGVNIADKMITYIERKIR
>Mature_296_residues
MKKTGWIIVNGNLKTDKFIDYAKMIQAAAIKRHIDISIMKNNDILLDLSDKQPLHLHKQTYTLPDFAFFIDKDIYLAQYL
EQLGIPVFNSSSTIEMSDDKIQTYQRLTSLDIPIPHTIIAPKIYTKGVLSDDYLQSIIHKLGIPLVVKEAFGSFGEQVYL
VNNVEELKELTNNLQGKPFLFQSYISSSHGRDIRLQVVGNEVIAGMERNNNQDFRANITNGGDMKPFTPDQKAINIAVDA
AQAINADFAGVDLLFGPENSYFVCEINANAHIRNLTECTGVNIADKMITYIERKIR

Specific function: Responsible For The Addition Of Glutamate Residues To The C-Terminus Of Ribosomal Protein S6. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=203, Percent_Identity=32.0197044334975, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI134254462, Length=203, Percent_Identity=29.5566502463054, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787076, Length=298, Percent_Identity=31.5436241610738, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33387; Mature: 33387

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTGWIIVNGNLKTDKFIDYAKMIQAAAIKRHIDISIMKNNDILLDLSDKQPLHLHKQT
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEECCCCCEEEEEEE
YTLPDFAFFIDKDIYLAQYLEQLGIPVFNSSSTIEMSDDKIQTYQRLTSLDIPIPHTIIA
CCCCCEEEECCCCHHHHHHHHHHCCEEECCCCEEEECCHHHHHHHHHHHCCCCCCCEEEC
PKIYTKGVLSDDYLQSIIHKLGIPLVVKEAFGSFGEQVYLVNNVEELKELTNNLQGKPFL
CCHHHCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCH
FQSYISSSHGRDIRLQVVGNEVIAGMERNNNQDFRANITNGGDMKPFTPDQKAINIAVDA
HHHHHCCCCCCEEEEEEECCHHEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEH
AQAINADFAGVDLLFGPENSYFVCEINANAHIRNLTECTGVNIADKMITYIERKIR
HHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTGWIIVNGNLKTDKFIDYAKMIQAAAIKRHIDISIMKNNDILLDLSDKQPLHLHKQT
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEECCCCCEEEEEEE
YTLPDFAFFIDKDIYLAQYLEQLGIPVFNSSSTIEMSDDKIQTYQRLTSLDIPIPHTIIA
CCCCCEEEECCCCHHHHHHHHHHCCEEECCCCEEEECCHHHHHHHHHHHCCCCCCCEEEC
PKIYTKGVLSDDYLQSIIHKLGIPLVVKEAFGSFGEQVYLVNNVEELKELTNNLQGKPFL
CCHHHCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCH
FQSYISSSHGRDIRLQVVGNEVIAGMERNNNQDFRANITNGGDMKPFTPDQKAINIAVDA
HHHHHCCCCCCEEEEEEECCHHEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEH
AQAINADFAGVDLLFGPENSYFVCEINANAHIRNLTECTGVNIADKMITYIERKIR
HHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]