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The map label for this gene is mtnN

Identifier: 23099455

GI number: 23099455

Start: 2024930

End: 2025619

Strand: Reverse

Name: mtnN

Synonym: OB2000

Alternate gene names: 23099455

Gene position: 2025619-2024930 (Counterclockwise)

Preceding gene: 23099456

Following gene: 23099444

Centisome position: 55.79

GC content: 36.67

Gene sequence:

>690_bases
ATGACAATAGGAATTATTGGAGCAATGGACGAAGAGATTGCACTTTTAAAACAACAAATGAAAGATATGGAAGAATTAGA
GGTAGCAGGTTGCTACTTTTATACAGGGCATCTACATGACAAGAGTGTCGTATTACTATTATCGGGTATCGGAAAAGTAA
ATGCTGCTATGGCTACAACAATTCTACATGAGCGATTTTCCCCATCTATGATTATAAATACTGGTTCTGCAGGTGGGTTT
GCTAGCGATTTACAAGTAGGAGACGTAGTAATTTCTGAAGAAGTATTGCATCATGATGTGGACGCAACAGCGTTTGATTA
TGTATATGGACAAGTTCCTGGTATGCCTGCAACGTATAAAGCAGATCAACGACTAGTTGAATTGTCCTCTGAAGTTATGA
AGGATATAGAAATTAACTCAAGAATCGGTGTTATTGCAACAGGTGATTCATTTATGCAACGAAAGGATCAAACGGATATT
GTTAAACAACGATTTCCAGGTATGCTAGCGTTAGAGATGGAAGCAGCCTCTATCGCACAAGTATGTTATAGATACAATAC
GCCTTTTGTTATTACTCGTGCATTATCTGATATTGCAGGACAAGAATCCTCTGTATCGTTTGATCAGTTTTTACAGACAG
CTGGTAAAAATGCCGCTCAATTAATTATAAATATGGTAAAAAAAATATAA

Upstream 100 bases:

>100_bases
GTTTCGTGTATACCTTCAATGGGTTGATGGTGAAGGTTGGCAGCCTACCAAAGTAGAAGAGTTAATTGAGAATGATAAAA
AATAGTTTTTGGGGGTCACT

Downstream 100 bases:

>100_bases
TTTAATAAACACTACAAAACGCAGCCAAATAATCTCAATTAATTTGGCTGCGTTTTGCATTTTACATAAAATGTGTTTAT
GCAGTGATACTTAAAAAATT

Product: 5'-methylthioadenosine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTIGIIGAMDEEIALLKQQMKDMEELEVAGCYFYTGHLHDKSVVLLLSGIGKVNAAMATTILHERFSPSMIINTGSAGGF
ASDLQVGDVVISEEVLHHDVDATAFDYVYGQVPGMPATYKADQRLVELSSEVMKDIEINSRIGVIATGDSFMQRKDQTDI
VKQRFPGMLALEMEAASIAQVCYRYNTPFVITRALSDIAGQESSVSFDQFLQTAGKNAAQLIINMVKKI

Sequences:

>Translated_229_residues
MTIGIIGAMDEEIALLKQQMKDMEELEVAGCYFYTGHLHDKSVVLLLSGIGKVNAAMATTILHERFSPSMIINTGSAGGF
ASDLQVGDVVISEEVLHHDVDATAFDYVYGQVPGMPATYKADQRLVELSSEVMKDIEINSRIGVIATGDSFMQRKDQTDI
VKQRFPGMLALEMEAASIAQVCYRYNTPFVITRALSDIAGQESSVSFDQFLQTAGKNAAQLIINMVKKI
>Mature_228_residues
TIGIIGAMDEEIALLKQQMKDMEELEVAGCYFYTGHLHDKSVVLLLSGIGKVNAAMATTILHERFSPSMIINTGSAGGFA
SDLQVGDVVISEEVLHHDVDATAFDYVYGQVPGMPATYKADQRLVELSSEVMKDIEINSRIGVIATGDSFMQRKDQTDIV
KQRFPGMLALEMEAASIAQVCYRYNTPFVITRALSDIAGQESSVSFDQFLQTAGKNAAQLIINMVKKI

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=229, Percent_Identity=45.8515283842795, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_OCEIH (Q8EPT8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692921.1
- HSSP:   P0AF12
- ProteinModelPortal:   Q8EPT8
- SMR:   Q8EPT8
- GeneID:   1018481
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2000
- NMPDR:   fig|221109.1.peg.1999
- HOGENOM:   HBG367723
- OMA:   AMEQEVT
- BioCyc:   OIHE221109:OB2000-MONOMER
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 25035; Mature: 24904

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGIIGAMDEEIALLKQQMKDMEELEVAGCYFYTGHLHDKSVVLLLSGIGKVNAAMATT
CEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHEEEECCCCHHHHHHHHH
ILHERFSPSMIINTGSAGGFASDLQVGDVVISEEVLHHDVDATAFDYVYGQVPGMPATYK
HHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHH
ADQRLVELSSEVMKDIEINSRIGVIATGDSFMQRKDQTDIVKQRFPGMLALEMEAASIAQ
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHHHHHCCCEEEEEHHHHHHHH
VCYRYNTPFVITRALSDIAGQESSVSFDQFLQTAGKNAAQLIINMVKKI
HHHHCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHC
>Mature Secondary Structure 
TIGIIGAMDEEIALLKQQMKDMEELEVAGCYFYTGHLHDKSVVLLLSGIGKVNAAMATT
EEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHEEEECCCCHHHHHHHHH
ILHERFSPSMIINTGSAGGFASDLQVGDVVISEEVLHHDVDATAFDYVYGQVPGMPATYK
HHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHH
ADQRLVELSSEVMKDIEINSRIGVIATGDSFMQRKDQTDIVKQRFPGMLALEMEAASIAQ
HHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHHHHHCCCEEEEEHHHHHHHH
VCYRYNTPFVITRALSDIAGQESSVSFDQFLQTAGKNAAQLIINMVKKI
HHHHCCCCEEHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376