Click here to switch to the map view.

The map label for this gene is murE2

Identifier: 23099453

GI number: 23099453

Start: 2022367

End: 2023845

Strand: Direct

Name: murE2

Synonym: OB1998

Alternate gene names: 23099453

Gene position: 2022367-2023845 (Clockwise)

Preceding gene: 23099452

Following gene: 23099454

Centisome position: 55.7

GC content: 33.4

Gene sequence:

>1479_bases
TTGAATACTTATGAACTTATTTCATCCTTAAAAATAAAACAACAAATAGGTAAATTACCTAATACTATTTCCTCAATTTC
ACACAATTCTCGTCAAGTGGACGATAACTCCTTATTTATTTGTATAAGTGGCTTTACTGTAGATGGACATAATTTTGTAG
CCGAGGCTGTAGAGAATGGTGCAACGTGTATTGTAGCAGAAAGAGAAGTTAATCTCGATACAAATAAAGCGACTTTAATT
ATAGTTCCCGATACAAATAAAGCTATAGCAACACTTGCTGCTACTTTTTATAAACATCCATCTCATAATTTAACGATATA
CGGTGTTACAGGAACAAATGGAAAAACTACAGTGACAACCATTATTAATCATTTATTAACGGATAATGAACTTTCTACTG
CCCTGATTGGAACGAATGGATTCCAGATAAATAAAGATATCTATTCTAGCAGTAATAATACAACAAGTGATGTTTTAACA
AATCAACATATGCTATATGAAGCAAAAAATCAAGGTGTGACACATGTGGCAATGGAAGTATCTTCACATGGATTAGAACA
AGGCCGTCTATGGGGGATTGATTTTACCGTTGCTACCTTCACCAACCTGTCCCATGAACATTTGGATTATCATGGTAGTA
TGGAAGAATATGCTGCTACCAAGGTGCAATTATTTAGTTCGCTAGCAATTCAACCTAATAAACAAAAAATTGCAGTGTTA
AATCGAGATGATAATTGGTACGAATATTTTCAGAAACGAACGCCTGTACAAATCGTTTCGTATGGAATTTATGAACAGGC
TGATTTCAGGGCTACCGCTATAAAATATTATAAAGACAGAACTTCATTTGAACTATTAACACCAGAGGGAAGCTTTCAAG
TACAAACAAAGCTTATGGGAGAGTTTAATGTTTATAATGTTTTAGCAGCGATCGCATCCGTTTATGTAAAAGAATCAATT
CCTTTAACAGATTTAGTTAAATCAATTGAAGGGACTCCTACCCCACCTGGAAGAATGGAAAAATTAGAAAGTAGTGGAAA
TAGACATATCTATATTGACTATGCTCATACTCCAGATGCACTAACAAAGGCAATTAATAGTTTACTTCCTTTTAAACAAA
ACAAATTAATTGTTGTTGTAGGAACAGGTGGTGATAGAGACAAAAGTATACGTCCGTTATTAGCCAAAGCTGCATCTGTC
GCAGATTATGTTATATTAACGATTAATGATCCAAGACATGAAGATCCGAATAATATATTACAAGACCTTGAAAACGGAAT
GTTACATCATCAATATACGTGTCTTGAAAATCGAAAAGAAGCTATTCGTGAGGCAATAAATTATTCTTCTCCCAACGATA
TTATTTTAATTGCAGGGAAAGGAAAAGAGCCATACCAGATTATTAAAAATAAGAAGGTAGCTCATAATGATGCTACTATT
GCACTTGAATGCAGTAATGAATTATTTCCAAATATATGA

Upstream 100 bases:

>100_bases
TTGGATTCTTTTTTCTATATTTTTCAGATTAATAAATATGGAAATTGGAAATATTACTAACAACTTAAATATGATAACTT
AAAAATAAGGAGACATCACC

Downstream 100 bases:

>100_bases
ATGAATTTCTTTAGAGGTTTTATATCCAATATTACTCTGGCTAATTCCCGTTAACCAAGGAACTCTTTAGCGTAGATTTT
TTTTAATGCATCGATTTTAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase 2

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNTYELISSLKIKQQIGKLPNTISSISHNSRQVDDNSLFICISGFTVDGHNFVAEAVENGATCIVAEREVNLDTNKATLI
IVPDTNKAIATLAATFYKHPSHNLTIYGVTGTNGKTTVTTIINHLLTDNELSTALIGTNGFQINKDIYSSSNNTTSDVLT
NQHMLYEAKNQGVTHVAMEVSSHGLEQGRLWGIDFTVATFTNLSHEHLDYHGSMEEYAATKVQLFSSLAIQPNKQKIAVL
NRDDNWYEYFQKRTPVQIVSYGIYEQADFRATAIKYYKDRTSFELLTPEGSFQVQTKLMGEFNVYNVLAAIASVYVKESI
PLTDLVKSIEGTPTPPGRMEKLESSGNRHIYIDYAHTPDALTKAINSLLPFKQNKLIVVVGTGGDRDKSIRPLLAKAASV
ADYVILTINDPRHEDPNNILQDLENGMLHHQYTCLENRKEAIREAINYSSPNDIILIAGKGKEPYQIIKNKKVAHNDATI
ALECSNELFPNI

Sequences:

>Translated_492_residues
MNTYELISSLKIKQQIGKLPNTISSISHNSRQVDDNSLFICISGFTVDGHNFVAEAVENGATCIVAEREVNLDTNKATLI
IVPDTNKAIATLAATFYKHPSHNLTIYGVTGTNGKTTVTTIINHLLTDNELSTALIGTNGFQINKDIYSSSNNTTSDVLT
NQHMLYEAKNQGVTHVAMEVSSHGLEQGRLWGIDFTVATFTNLSHEHLDYHGSMEEYAATKVQLFSSLAIQPNKQKIAVL
NRDDNWYEYFQKRTPVQIVSYGIYEQADFRATAIKYYKDRTSFELLTPEGSFQVQTKLMGEFNVYNVLAAIASVYVKESI
PLTDLVKSIEGTPTPPGRMEKLESSGNRHIYIDYAHTPDALTKAINSLLPFKQNKLIVVVGTGGDRDKSIRPLLAKAASV
ADYVILTINDPRHEDPNNILQDLENGMLHHQYTCLENRKEAIREAINYSSPNDIILIAGKGKEPYQIIKNKKVAHNDATI
ALECSNELFPNI
>Mature_492_residues
MNTYELISSLKIKQQIGKLPNTISSISHNSRQVDDNSLFICISGFTVDGHNFVAEAVENGATCIVAEREVNLDTNKATLI
IVPDTNKAIATLAATFYKHPSHNLTIYGVTGTNGKTTVTTIINHLLTDNELSTALIGTNGFQINKDIYSSSNNTTSDVLT
NQHMLYEAKNQGVTHVAMEVSSHGLEQGRLWGIDFTVATFTNLSHEHLDYHGSMEEYAATKVQLFSSLAIQPNKQKIAVL
NRDDNWYEYFQKRTPVQIVSYGIYEQADFRATAIKYYKDRTSFELLTPEGSFQVQTKLMGEFNVYNVLAAIASVYVKESI
PLTDLVKSIEGTPTPPGRMEKLESSGNRHIYIDYAHTPDALTKAINSLLPFKQNKLIVVVGTGGDRDKSIRPLLAKAASV
ADYVILTINDPRHEDPNNILQDLENGMLHHQYTCLENRKEAIREAINYSSPNDIILIAGKGKEPYQIIKNKKVAHNDATI
ALECSNELFPNI

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=478, Percent_Identity=34.1004184100418, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786274, Length=379, Percent_Identity=25.065963060686, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE2_OCEIH (Q8CZE4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692919.1
- ProteinModelPortal:   Q8CZE4
- SMR:   Q8CZE4
- GeneID:   1018478
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1998
- NMPDR:   fig|221109.1.peg.1997
- HOGENOM:   HBG602753
- OMA:   EDYLAHK
- BioCyc:   OIHE221109:OB1998-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: 6.3.2.13

Molecular weight: Translated: 54714; Mature: 54714

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 181-181 BINDING 187-187 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTYELISSLKIKQQIGKLPNTISSISHNSRQVDDNSLFICISGFTVDGHNFVAEAVENG
CCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHCC
ATCIVAEREVNLDTNKATLIIVPDTNKAIATLAATFYKHPSHNLTIYGVTGTNGKTTVTT
CEEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHH
IINHLLTDNELSTALIGTNGFQINKDIYSSSNNTTSDVLTNQHMLYEAKNQGVTHVAMEV
HHHHHHCCCCCCEEEEECCCEEECHHHHCCCCCCHHHHHCCCCEEEEECCCCEEEEEEEH
SSHGLEQGRLWGIDFTVATFTNLSHEHLDYHGSMEEYAATKVQLFSSLAIQPNKQKIAVL
HHCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
NRDDNWYEYFQKRTPVQIVSYGIYEQADFRATAIKYYKDRTSFELLTPEGSFQVQTKLMG
ECCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEEEECC
EFNVYNVLAAIASVYVKESIPLTDLVKSIEGTPTPPGRMEKLESSGNRHIYIDYAHTPDA
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCHH
LTKAINSLLPFKQNKLIVVVGTGGDRDKSIRPLLAKAASVADYVILTINDPRHEDPNNIL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
QDLENGMLHHQYTCLENRKEAIREAINYSSPNDIILIAGKGKEPYQIIKNKKVAHNDATI
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEECCCCEE
ALECSNELFPNI
EEEECCCCCCCC
>Mature Secondary Structure
MNTYELISSLKIKQQIGKLPNTISSISHNSRQVDDNSLFICISGFTVDGHNFVAEAVENG
CCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHCC
ATCIVAEREVNLDTNKATLIIVPDTNKAIATLAATFYKHPSHNLTIYGVTGTNGKTTVTT
CEEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHH
IINHLLTDNELSTALIGTNGFQINKDIYSSSNNTTSDVLTNQHMLYEAKNQGVTHVAMEV
HHHHHHCCCCCCEEEEECCCEEECHHHHCCCCCCHHHHHCCCCEEEEECCCCEEEEEEEH
SSHGLEQGRLWGIDFTVATFTNLSHEHLDYHGSMEEYAATKVQLFSSLAIQPNKQKIAVL
HHCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
NRDDNWYEYFQKRTPVQIVSYGIYEQADFRATAIKYYKDRTSFELLTPEGSFQVQTKLMG
ECCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEEEECC
EFNVYNVLAAIASVYVKESIPLTDLVKSIEGTPTPPGRMEKLESSGNRHIYIDYAHTPDA
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCHH
LTKAINSLLPFKQNKLIVVVGTGGDRDKSIRPLLAKAASVADYVILTINDPRHEDPNNIL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
QDLENGMLHHQYTCLENRKEAIREAINYSSPNDIILIAGKGKEPYQIIKNKKVAHNDATI
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEECCCCEE
ALECSNELFPNI
EEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376