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The map label for this gene is aroE

Identifier: 23099442

GI number: 23099442

Start: 2013167

End: 2013997

Strand: Reverse

Name: aroE

Synonym: OB1987

Alternate gene names: 23099442

Gene position: 2013997-2013167 (Counterclockwise)

Preceding gene: 23099443

Following gene: 23099441

Centisome position: 55.47

GC content: 34.9

Gene sequence:

>831_bases
TTGACGTTATCTTTAAAATTGATTGGTTATCCAATAGAACATTCAATGTCACCATGGATTCATAATGAATTTCTAAAACG
TTCAAATCTTGAAGGTACGTATGAACTTTTTGAAATAAGCCCGGAAGAAAGCTTTGAAGATAATGTAACAACACTCAAAA
AATCTGTCTTGACGGGTTTTAATGTTACGGTACCTTATAAACAAAAGATTATGCAATTCTTAGATGAAGTTGATGATACA
GCAAATTTAATGGGGGCAGTAAATACGGTATCTATTAGAGACGGGAAATGGATTGGATATAATACAGATGGTATAGGTTA
TTTACGATCTTTATATGCAGCATATCCTTTCTTAAAGGGAGTAACGAATAAGCGTGTATTAATCCTTGGAGCTGGTGGAG
CGGCGCGTGGTATTTTCCATGCCCTAGTAAATGAAGGCTATAATAATATTAAAATTGCTAACCGGACTTTAAGCCGAGCT
GAATCAATAATTGGAACAAATAAACAAGCTTTAGCAATCAGTTTGGAAGAAGCTGCAGAAGAGTTACATCAATTTGATTT
AGTTATTCAAACGTCTGCCGTTGGAATGAATGAACCACGCTCGATTATTATATTAGACAGGATTAATGAAGATACGGTTG
TTAGTGATATCGTATATCAACCGTTAGAAACCCATTTTCTGCAATTAGCAAAACAACGCACTCCCTATATTCACCATGGA
CACACTATGTTGTTATATCAAGCACAAGCAGCTTTTGAAATATGGACAGGAACAAATGTCAATGTATCCGGAATGGATAT
GCAAATCGAACAAATATTGAAAGGAAGATAA

Upstream 100 bases:

>100_bases
AGTGTGCAAGGAAAAGGAGCAACTGTACAAGCTCATACTTGGAAAGGCATTTCTATTACTGTACGCAAGTCACTTATTTA
AGATGTGGGAGGGGAGAACG

Downstream 100 bases:

>100_bases
TAATGCTAACAGGAAAACAAAGAAGATTTTTACGAGCTCAAGCAAATCAACTTAAACCTATTTTTCAAGTCGGGAAAGAT
GGGGTTAATGATAATATGGT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTLSLKLIGYPIEHSMSPWIHNEFLKRSNLEGTYELFEISPEESFEDNVTTLKKSVLTGFNVTVPYKQKIMQFLDEVDDT
ANLMGAVNTVSIRDGKWIGYNTDGIGYLRSLYAAYPFLKGVTNKRVLILGAGGAARGIFHALVNEGYNNIKIANRTLSRA
ESIIGTNKQALAISLEEAAEELHQFDLVIQTSAVGMNEPRSIIILDRINEDTVVSDIVYQPLETHFLQLAKQRTPYIHHG
HTMLLYQAQAAFEIWTGTNVNVSGMDMQIEQILKGR

Sequences:

>Translated_276_residues
MTLSLKLIGYPIEHSMSPWIHNEFLKRSNLEGTYELFEISPEESFEDNVTTLKKSVLTGFNVTVPYKQKIMQFLDEVDDT
ANLMGAVNTVSIRDGKWIGYNTDGIGYLRSLYAAYPFLKGVTNKRVLILGAGGAARGIFHALVNEGYNNIKIANRTLSRA
ESIIGTNKQALAISLEEAAEELHQFDLVIQTSAVGMNEPRSIIILDRINEDTVVSDIVYQPLETHFLQLAKQRTPYIHHG
HTMLLYQAQAAFEIWTGTNVNVSGMDMQIEQILKGR
>Mature_275_residues
TLSLKLIGYPIEHSMSPWIHNEFLKRSNLEGTYELFEISPEESFEDNVTTLKKSVLTGFNVTVPYKQKIMQFLDEVDDTA
NLMGAVNTVSIRDGKWIGYNTDGIGYLRSLYAAYPFLKGVTNKRVLILGAGGAARGIFHALVNEGYNNIKIANRTLSRAE
SIIGTNKQALAISLEEAAEELHQFDLVIQTSAVGMNEPRSIIILDRINEDTVVSDIVYQPLETHFLQLAKQRTPYIHHGH
TMLLYQAQAAFEIWTGTNVNVSGMDMQIEQILKGR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=273, Percent_Identity=33.6996336996337, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787983, Length=269, Percent_Identity=32.3420074349442, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320332, Length=269, Percent_Identity=29.368029739777, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_OCEIH (Q8EPU9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692908.1
- HSSP:   Q58484
- ProteinModelPortal:   Q8EPU9
- SMR:   Q8EPU9
- GeneID:   1018464
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1987
- NMPDR:   fig|221109.1.peg.1987
- HOGENOM:   HBG553408
- OMA:   APRISIV
- BioCyc:   OIHE221109:OB1987-MONOMER
- BRENDA:   1.1.1.25
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30966; Mature: 30835

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSLKLIGYPIEHSMSPWIHNEFLKRSNLEGTYELFEISPEESFEDNVTTLKKSVLTGF
CEEEEEEEECCHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
NVTVPYKQKIMQFLDEVDDTANLMGAVNTVSIRDGKWIGYNTDGIGYLRSLYAAYPFLKG
CEECCHHHHHHHHHHHCCHHHHHHHCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHC
VTNKRVLILGAGGAARGIFHALVNEGYNNIKIANRTLSRAESIIGTNKQALAISLEEAAE
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEHHHHHH
ELHQFDLVIQTSAVGMNEPRSIIILDRINEDTVVSDIVYQPLETHFLQLAKQRTPYIHHG
HHHHHHEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECC
HTMLLYQAQAAFEIWTGTNVNVSGMDMQIEQILKGR
CEEEEEEECEEEEEECCCCCEECCCCHHHHHHHCCC
>Mature Secondary Structure 
TLSLKLIGYPIEHSMSPWIHNEFLKRSNLEGTYELFEISPEESFEDNVTTLKKSVLTGF
EEEEEEEECCHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
NVTVPYKQKIMQFLDEVDDTANLMGAVNTVSIRDGKWIGYNTDGIGYLRSLYAAYPFLKG
CEECCHHHHHHHHHHHCCHHHHHHHCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHC
VTNKRVLILGAGGAARGIFHALVNEGYNNIKIANRTLSRAESIIGTNKQALAISLEEAAE
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEHHHHHH
ELHQFDLVIQTSAVGMNEPRSIIILDRINEDTVVSDIVYQPLETHFLQLAKQRTPYIHHG
HHHHHHEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECC
HTMLLYQAQAAFEIWTGTNVNVSGMDMQIEQILKGR
CEEEEEEECEEEEEECCCCCEECCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376