Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 23099281

GI number: 23099281

Start: 1861779

End: 1862882

Strand: Reverse

Name: dacB [H]

Synonym: OB1826

Alternate gene names: 23099281

Gene position: 1862882-1861779 (Counterclockwise)

Preceding gene: 23099282

Following gene: 23099280

Centisome position: 51.31

GC content: 34.24

Gene sequence:

>1104_bases
TTGATGCGTGTATTCATAGGATTATTAACATCATTAATATTATTCATGAGTTTTCCTTCGTATGGACAAGCATTGCCGTC
TGTATCAGCAAATAACGCAGTATTAATTGAACAATCAACAGGCAGAGTGTTATTTGAGAAAAATGCAAACGAAGAAGCTT
CCATAGCAAGCATTACAAAAATCATGACAGCAATTATTGCTATAGAATCTGGGATGTTAGATGAACAAGTAACTACTTCG
AGAAAAGCAATTTATACTGAAGGATCCTCTATATATCTAGAACAAGGAGAAGAAATTCCTCTAAAAGATTTAGTTTATGG
ATTAATGCTTCGATCAGGAAATGATGCAGCGGTAGCAATAGCTGAACATGTTGGAGGTAGTGAAGAAGGATTTGTTTACT
TGATGAACGAAAAAGCCAAGTGGTTAGGAATGGAAAACACGCATTTTGATAATCCTCATGGTTTGGATTCAGACACACAT
TACTCTAGTGCATATGATATGGCAATTCTTATGAAATATGCAATGGATAATCCGATGTTTAGAGAGGTTAGTCAATCCGA
TTCATATCTTTCGAATAACAGAACCTATCATTGGCAAAACAAAAATAAACTGTTGACTCGTTTATATGAATATTGTACAG
GCGGTAAGACTGGTTATACGAAGGCTACTGGACGAACATTAGTCACTTCAGCTCATAAAAATGGAATGGATTTAATTGCA
GTTACTTTAGATGCTCCAGATGATTGGCAAGATCATATTGGTATGTTTGAGTGGGGATTTGAACAATTCGAAATGAAAAC
AGTTCAAGAAGAAGGTACGGCACTTTATCATATAGAACCTAAGACAAGTAAAGTATATGGGAATTATAATAAAGCTGTAA
ACCTCCCATTATTAAAGGACGGTAATGACAATGTAGATGTGAAGAATTATTTTTTATCCGATGTACGTAGTGATTCAAAA
CAAGCGATTGGGAAATCTGTTTATTATATCAACAATCAAGAAGTGTATGAATCGTATATTTATCCAGGAGAACAACCGAA
TAACTCTATCTTTGATAACCTAAAAACCGTATTTGAATCCATCATTGGAAGAGAATCAATATGA

Upstream 100 bases:

>100_bases
ATCTTCTTGCAGAATTATCAAATCTCTTAAATAAAGAATGACATAACCATTTCTGACTTGCATAAGATAATTATAGAAAA
GAATTTTAGGAGGACAAGAA

Downstream 100 bases:

>100_bases
TCAATTGGATTTGGATCTCCATGGCAGTAATTGGGATCGTTTATGCTATGTTTAGCGGAAATATGGAAGAGGTAAATCAA
GCAATCTTTGAAAGTGCTGG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MMRVFIGLLTSLILFMSFPSYGQALPSVSANNAVLIEQSTGRVLFEKNANEEASIASITKIMTAIIAIESGMLDEQVTTS
RKAIYTEGSSIYLEQGEEIPLKDLVYGLMLRSGNDAAVAIAEHVGGSEEGFVYLMNEKAKWLGMENTHFDNPHGLDSDTH
YSSAYDMAILMKYAMDNPMFREVSQSDSYLSNNRTYHWQNKNKLLTRLYEYCTGGKTGYTKATGRTLVTSAHKNGMDLIA
VTLDAPDDWQDHIGMFEWGFEQFEMKTVQEEGTALYHIEPKTSKVYGNYNKAVNLPLLKDGNDNVDVKNYFLSDVRSDSK
QAIGKSVYYINNQEVYESYIYPGEQPNNSIFDNLKTVFESIIGRESI

Sequences:

>Translated_367_residues
MMRVFIGLLTSLILFMSFPSYGQALPSVSANNAVLIEQSTGRVLFEKNANEEASIASITKIMTAIIAIESGMLDEQVTTS
RKAIYTEGSSIYLEQGEEIPLKDLVYGLMLRSGNDAAVAIAEHVGGSEEGFVYLMNEKAKWLGMENTHFDNPHGLDSDTH
YSSAYDMAILMKYAMDNPMFREVSQSDSYLSNNRTYHWQNKNKLLTRLYEYCTGGKTGYTKATGRTLVTSAHKNGMDLIA
VTLDAPDDWQDHIGMFEWGFEQFEMKTVQEEGTALYHIEPKTSKVYGNYNKAVNLPLLKDGNDNVDVKNYFLSDVRSDSK
QAIGKSVYYINNQEVYESYIYPGEQPNNSIFDNLKTVFESIIGRESI
>Mature_367_residues
MMRVFIGLLTSLILFMSFPSYGQALPSVSANNAVLIEQSTGRVLFEKNANEEASIASITKIMTAIIAIESGMLDEQVTTS
RKAIYTEGSSIYLEQGEEIPLKDLVYGLMLRSGNDAAVAIAEHVGGSEEGFVYLMNEKAKWLGMENTHFDNPHGLDSDTH
YSSAYDMAILMKYAMDNPMFREVSQSDSYLSNNRTYHWQNKNKLLTRLYEYCTGGKTGYTKATGRTLVTSAHKNGMDLIA
VTLDAPDDWQDHIGMFEWGFEQFEMKTVQEEGTALYHIEPKTSKVYGNYNKAVNLPLLKDGNDNVDVKNYFLSDVRSDSK
QAIGKSVYYINNQEVYESYIYPGEQPNNSIFDNLKTVFESIIGRESI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=277, Percent_Identity=36.101083032491, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786851, Length=256, Percent_Identity=35.15625, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082022, Length=262, Percent_Identity=32.824427480916, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082054, Length=231, Percent_Identity=30.7359307359307, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41272; Mature: 41272

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRVFIGLLTSLILFMSFPSYGQALPSVSANNAVLIEQSTGRVLFEKNANEEASIASITK
CHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHH
IMTAIIAIESGMLDEQVTTSRKAIYTEGSSIYLEQGEEIPLKDLVYGLMLRSGNDAAVAI
HHHHHHHHHCCCCCHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEE
AEHVGGSEEGFVYLMNEKAKWLGMENTHFDNPHGLDSDTHYSSAYDMAILMKYAMDNPMF
EHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
REVSQSDSYLSNNRTYHWQNKNKLLTRLYEYCTGGKTGYTKATGRTLVTSAHKNGMDLIA
HHHHHCCCHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEE
VTLDAPDDWQDHIGMFEWGFEQFEMKTVQEEGTALYHIEPKTSKVYGNYNKAVNLPLLKD
EEECCCCCHHHHCCHHHCCHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECEEEEC
GNDNVDVKNYFLSDVRSDSKQAIGKSVYYINNQEVYESYIYPGEQPNNSIFDNLKTVFES
CCCCCCHHHHHHHHHCCCHHHHHCCEEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IIGRESI
HHCCCCC
>Mature Secondary Structure
MMRVFIGLLTSLILFMSFPSYGQALPSVSANNAVLIEQSTGRVLFEKNANEEASIASITK
CHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHH
IMTAIIAIESGMLDEQVTTSRKAIYTEGSSIYLEQGEEIPLKDLVYGLMLRSGNDAAVAI
HHHHHHHHHCCCCCHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEE
AEHVGGSEEGFVYLMNEKAKWLGMENTHFDNPHGLDSDTHYSSAYDMAILMKYAMDNPMF
EHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
REVSQSDSYLSNNRTYHWQNKNKLLTRLYEYCTGGKTGYTKATGRTLVTSAHKNGMDLIA
HHHHHCCCHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEE
VTLDAPDDWQDHIGMFEWGFEQFEMKTVQEEGTALYHIEPKTSKVYGNYNKAVNLPLLKD
EEECCCCCHHHHCCHHHCCHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECEEEEC
GNDNVDVKNYFLSDVRSDSKQAIGKSVYYINNQEVYESYIYPGEQPNNSIFDNLKTVFES
CCCCCCHHHHHHHHHCCCHHHHHCCEEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IIGRESI
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]