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The map label for this gene is aroF

Identifier: 23099240

GI number: 23099240

Start: 1822335

End: 1823504

Strand: Reverse

Name: aroF

Synonym: OB1785

Alternate gene names: 23099240

Gene position: 1823504-1822335 (Counterclockwise)

Preceding gene: 23099241

Following gene: 23099239

Centisome position: 50.23

GC content: 40.34

Gene sequence:

>1170_bases
TTGCGATACATAACATCTGGCGAATCTCATGGTAAACAATTAACAACGATAATCGAAGGTCTACCTTCACAATTACCAAT
TACTGCAGAAGATATTAACGCTTCTTTACGTCGAAGACAAGGTGGACACGGTAGAGGGAAGCGTATGCAAATAGAGAAAG
ATACAGTAGATATTGTAAGTGGTGTTCGTCATGGGTATACATTAGGATCTCCACTATCATTAGTAATCCGCAATGATGAT
TTTAAACATTGGACAGATATTATGGGCGAAGAACCAATGGAAGATCCTTCAAAAATGCGCCGTGTAGTTACAAAACCAAG
ACCTGGACATGCAGATCTAAACGGTGCACTTAAATACGGACATAGAGATATGAGAAACGTATTGGAACGTTCTTCTGCAA
GAGAAACGGCCGCTCGTGTTGCTGCGGGTGCTGTTGCTAAAAAACTACTAAAAGAACTAGGAATTGAAGTTAGCGGTTAT
GTAAAGGAAATTGCTGGGATAGAAGCTAGTGATCTACCAGAATTAAACGCACAAGAACGTGCAGATAAGGCAAACCAATC
ACCAGTAATGGTTCTAGACGCTACTGTTGAAGAGCAAATGACAAATGCAATCGATCAAGCTAAAAAAGAAGGAGATTCCA
TCGGTGGTGTCTGTGAAGTATATGTAGAAGGGATGCCAGCAGGAGTTGGATCTTATGTACACTATGATCGGAAGTTAGAT
AGTAGATTAGCAGGCAGTGTCGTAAGTATTAATGCCTTTAAGGGTGTTGAATTCGGAATCGGATTTGAAGCGGCGAAACG
TAATGGAAGTGAAGTGCATGATGAAATAGCATGGGATGAAACGCATGGTTACTACCGTACTACAAATAGATTAGGTGGTT
TTGAAGGTGGAATGACGACTGGGATGCCTATTATTGTTCGAGGGGTTATGAAACCAATCCCAACACTCATGAAGAGACCG
TTAACTAGTGTTGATATCGAAACAAAAGAACCTTTTAAAGCTACAGTAGAACGGTCGGATGCATGTGCTGTTCCTGCAGC
ATCAGTAGTTATGGAACATGTAGTAGCATTCGAATTAGCAAAAGCCCTTACCGAACAATTTTCTAGCGATCAATTGCCAC
AATTAAAAAAAGCAATTGATGATTACAGAGAAGAAATTAGGTGCTTCTAA

Upstream 100 bases:

>100_bases
TAAGAAAATAAAATAAATCATGTGAAAGACTACCCCTAATAAACTTATCTATGATATAGTAATTAGAAAACTTTCAACTA
TTTAAGAAGGAGAATGTAAA

Downstream 100 bases:

>100_bases
AAATGGAAGAAATGACAGTGCAATCGAACCAAAGCTCGTACCCAATTTATATTGGTCAAGGCTTACGATATCAACTAAGT
TCCTATATTGAAAAAAAATA

Product: chorismate synthase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate phospholyase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRYITSGESHGKQLTTIIEGLPSQLPITAEDINASLRRRQGGHGRGKRMQIEKDTVDIVSGVRHGYTLGSPLSLVIRNDD
FKHWTDIMGEEPMEDPSKMRRVVTKPRPGHADLNGALKYGHRDMRNVLERSSARETAARVAAGAVAKKLLKELGIEVSGY
VKEIAGIEASDLPELNAQERADKANQSPVMVLDATVEEQMTNAIDQAKKEGDSIGGVCEVYVEGMPAGVGSYVHYDRKLD
SRLAGSVVSINAFKGVEFGIGFEAAKRNGSEVHDEIAWDETHGYYRTTNRLGGFEGGMTTGMPIIVRGVMKPIPTLMKRP
LTSVDIETKEPFKATVERSDACAVPAASVVMEHVVAFELAKALTEQFSSDQLPQLKKAIDDYREEIRCF

Sequences:

>Translated_389_residues
MRYITSGESHGKQLTTIIEGLPSQLPITAEDINASLRRRQGGHGRGKRMQIEKDTVDIVSGVRHGYTLGSPLSLVIRNDD
FKHWTDIMGEEPMEDPSKMRRVVTKPRPGHADLNGALKYGHRDMRNVLERSSARETAARVAAGAVAKKLLKELGIEVSGY
VKEIAGIEASDLPELNAQERADKANQSPVMVLDATVEEQMTNAIDQAKKEGDSIGGVCEVYVEGMPAGVGSYVHYDRKLD
SRLAGSVVSINAFKGVEFGIGFEAAKRNGSEVHDEIAWDETHGYYRTTNRLGGFEGGMTTGMPIIVRGVMKPIPTLMKRP
LTSVDIETKEPFKATVERSDACAVPAASVVMEHVVAFELAKALTEQFSSDQLPQLKKAIDDYREEIRCF
>Mature_389_residues
MRYITSGESHGKQLTTIIEGLPSQLPITAEDINASLRRRQGGHGRGKRMQIEKDTVDIVSGVRHGYTLGSPLSLVIRNDD
FKHWTDIMGEEPMEDPSKMRRVVTKPRPGHADLNGALKYGHRDMRNVLERSSARETAARVAAGAVAKKLLKELGIEVSGY
VKEIAGIEASDLPELNAQERADKANQSPVMVLDATVEEQMTNAIDQAKKEGDSIGGVCEVYVEGMPAGVGSYVHYDRKLD
SRLAGSVVSINAFKGVEFGIGFEAAKRNGSEVHDEIAWDETHGYYRTTNRLGGFEGGMTTGMPIIVRGVMKPIPTLMKRP
LTSVDIETKEPFKATVERSDACAVPAASVVMEHVVAFELAKALTEQFSSDQLPQLKKAIDDYREEIRCF

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; seventh step. [C]

COG id: COG0082

COG function: function code E; Chorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chorismate synthase family

Homologues:

Organism=Escherichia coli, GI1788669, Length=365, Percent_Identity=37.8082191780822, Blast_Score=164, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6321290, Length=379, Percent_Identity=34.0369393139842, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROC_OCEIH (Q8EQB6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692706.1
- ProteinModelPortal:   Q8EQB6
- SMR:   Q8EQB6
- GeneID:   1018279
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1785
- NMPDR:   fig|221109.1.peg.1786
- HOGENOM:   HBG292336
- OMA:   GSEAHDE
- ProtClustDB:   PRK05382
- BioCyc:   OIHE221109:OB1785-MONOMER
- BRENDA:   4.2.3.5
- HAMAP:   MF_00300_B
- InterPro:   IPR000453
- InterPro:   IPR020541
- PANTHER:   PTHR21085
- PIRSF:   PIRSF001456
- TIGRFAMs:   TIGR00033

Pfam domain/function: PF01264 Chorismate_synt; SSF103263 Chorismate_synth

EC number: =4.2.3.5

Molecular weight: Translated: 42684; Mature: 42684

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00787 CHORISMATE_SYNTHASE_1; PS00788 CHORISMATE_SYNTHASE_2; PS00789 CHORISMATE_SYNTHASE_3; PS00464 RIBOSOMAL_L22

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYITSGESHGKQLTTIIEGLPSQLPITAEDINASLRRRQGGHGRGKRMQIEKDTVDIVS
CCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHH
GVRHGYTLGSPLSLVIRNDDFKHWTDIMGEEPMEDPSKMRRVVTKPRPGHADLNGALKYG
HHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHC
HRDMRNVLERSSARETAARVAAGAVAKKLLKELGIEVSGYVKEIAGIEASDLPELNAQER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHH
ADKANQSPVMVLDATVEEQMTNAIDQAKKEGDSIGGVCEVYVEGMPAGVGSYVHYDRKLD
HHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
SRLAGSVVSINAFKGVEFGIGFEAAKRNGSEVHDEIAWDETHGYYRTTNRLGGFEGGMTT
HHHCCCEEEEECCCCCEECCCHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
GMPIIVRGVMKPIPTLMKRPLTSVDIETKEPFKATVERSDACAVPAASVVMEHVVAFELA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KALTEQFSSDQLPQLKKAIDDYREEIRCF
HHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRYITSGESHGKQLTTIIEGLPSQLPITAEDINASLRRRQGGHGRGKRMQIEKDTVDIVS
CCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHH
GVRHGYTLGSPLSLVIRNDDFKHWTDIMGEEPMEDPSKMRRVVTKPRPGHADLNGALKYG
HHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHC
HRDMRNVLERSSARETAARVAAGAVAKKLLKELGIEVSGYVKEIAGIEASDLPELNAQER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHH
ADKANQSPVMVLDATVEEQMTNAIDQAKKEGDSIGGVCEVYVEGMPAGVGSYVHYDRKLD
HHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
SRLAGSVVSINAFKGVEFGIGFEAAKRNGSEVHDEIAWDETHGYYRTTNRLGGFEGGMTT
HHHCCCEEEEECCCCCEECCCHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
GMPIIVRGVMKPIPTLMKRPLTSVDIETKEPFKATVERSDACAVPAASVVMEHVVAFELA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KALTEQFSSDQLPQLKKAIDDYREEIRCF
HHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376