BacMap

Definition	Oceanobacillus iheyensis HTE831, complete genome.
Accession	NC_004193
Length	3,630,528

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The map label for this gene is yngF [H]

Identifier: 23099153

GI number: 23099153

Start: 1739568

End: 1740347

Strand: Direct

Name: yngF [H]

Synonym: OB1698

Alternate gene names: 23099153

Gene position: 1739568-1740347 (Clockwise)

Preceding gene: 23099152

Following gene: 23099154

Centisome position: 47.92

GC content: 38.72

Gene sequence:

>780_bases
ATGGACTATTTAAACTATGAAATAGTGGAGAAGTATACTGGAATCATAACTTTACAGCGTCTTCAAGCGGCAAATGCGTT
ATCTGAAAGTCTCTTAAATGAATTAAATCAACTACTCGATGAAATCCAACAAAATGACACTATTCGTTGTGTGATTGTAA
CAGGCGCAGGAACAAAGGCTTTTTGTGCTGGTGCAGACTTAAAAGAGCGAAAAGGTATGAGTGATGATCAAGTGATTCAC
GCCGTTAAACAAATCGGTGAGACGATTAATCGAATAGAGAAGTTACAAGTACCAGTAATTGCTGCTATAAATGGGGTTGC
TCTCGGTGGTGGGTTAGAACTAGCGTTAGCATGCGACATTCGGATAGCCGTGAATAATACTAAGTTAGGATTAACAGAAA
CATCTTTAGCAATTATACCCGGAGCCGGAGGAACACAACGATTACCAAGGACAATTGGTGTCGGTCATGCTAAAAGACTT
ATATACTCTGCTGTTCCAGTAAATACCGAAGAAGCTTTACGTCTTCATTTGATTGAGCGATCGGTCAGCCAAGAGGAATT
ATTAGATGAAGCTGTTCAATTAGCAAAAAGAATTGCATCTAATGGACCGCTTGCTGTGCAGTTAGCAAAGGGTGTTATCA
ACAATGGATTAGATACAACCTTAGAAAATGGATTAGAAATAGAACATTTATCTTACCAGAAAGTAATACCTACTCATGAT
AGGCGAGAAGGTCTTCAAGCTTTTGCAGAAAAGAGAAAACCAGATTACCAAGGTAGATAA

Upstream 100 bases:

>100_bases
TCTGCGGGCACATTGAAGCAATGGAAAGTAAACGAAGGAGATTTTGTAAACGAAGGTGACGTTCTAGCAATCATAGAGTA
ATCGTTATTAGGAGGATTCG

Downstream 100 bases:

>100_bases
CAGTAAAGGGAGGAGCGAGTCTATGGATTATATTCAGGAATTACAACAACGAATAGAAAAAATAGAAAAAGGCGGAAAGC
AAAAGTATCATGACAGTAAC

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDYLNYEIVEKYTGIITLQRLQAANALSESLLNELNQLLDEIQQNDTIRCVIVTGAGTKAFCAGADLKERKGMSDDQVIH
AVKQIGETINRIEKLQVPVIAAINGVALGGGLELALACDIRIAVNNTKLGLTETSLAIIPGAGGTQRLPRTIGVGHAKRL
IYSAVPVNTEEALRLHLIERSVSQEELLDEAVQLAKRIASNGPLAVQLAKGVINNGLDTTLENGLEIEHLSYQKVIPTHD
RREGLQAFAEKRKPDYQGR

Sequences:

>Translated_259_residues
MDYLNYEIVEKYTGIITLQRLQAANALSESLLNELNQLLDEIQQNDTIRCVIVTGAGTKAFCAGADLKERKGMSDDQVIH
AVKQIGETINRIEKLQVPVIAAINGVALGGGLELALACDIRIAVNNTKLGLTETSLAIIPGAGGTQRLPRTIGVGHAKRL
IYSAVPVNTEEALRLHLIERSVSQEELLDEAVQLAKRIASNGPLAVQLAKGVINNGLDTTLENGLEIEHLSYQKVIPTHD
RREGLQAFAEKRKPDYQGR
>Mature_259_residues
MDYLNYEIVEKYTGIITLQRLQAANALSESLLNELNQLLDEIQQNDTIRCVIVTGAGTKAFCAGADLKERKGMSDDQVIH
AVKQIGETINRIEKLQVPVIAAINGVALGGGLELALACDIRIAVNNTKLGLTETSLAIIPGAGGTQRLPRTIGVGHAKRL
IYSAVPVNTEEALRLHLIERSVSQEELLDEAVQLAKRIASNGPLAVQLAKGVINNGLDTTLENGLEIEHLSYQKVIPTHD
RREGLQAFAEKRKPDYQGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=47.4509803921569, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=37.0656370656371, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=35.0806451612903, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI68989263, Length=185, Percent_Identity=38.3783783783784, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI20127408, Length=221, Percent_Identity=32.1266968325792, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=29.5719844357977, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=26.4227642276423, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI157694516, Length=220, Percent_Identity=28.6363636363636, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI213417737, Length=220, Percent_Identity=28.6363636363636, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI37594471, Length=144, Percent_Identity=32.6388888888889, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI37594469, Length=144, Percent_Identity=32.6388888888889, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=29, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI62530384, Length=231, Percent_Identity=27.7056277056277, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI261878539, Length=99, Percent_Identity=44.4444444444444, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI295842266, Length=229, Percent_Identity=25.764192139738, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=36.7588932806324, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=35.4581673306773, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=35.9375, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=29.5719844357977, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=29.4339622641509, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788682, Length=187, Percent_Identity=37.9679144385027, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790281, Length=207, Percent_Identity=31.8840579710145, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=40.5063291139241, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=40.9836065573771, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=36.1788617886179, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17558304, Length=222, Percent_Identity=31.981981981982, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17508953, Length=222, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17508951, Length=222, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=222, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=226, Percent_Identity=31.4159292035398, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=244, Percent_Identity=31.1475409836066, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=26.7924528301887, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=28.6919831223629, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=27.5, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=197, Percent_Identity=24.8730964467005, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=220, Percent_Identity=25.9090909090909, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=48.7903225806452, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=40.2298850574713, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=40.2298850574713, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=25.5905511811024, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=29.1828793774319, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=228, Percent_Identity=27.6315789473684, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583165, Length=222, Percent_Identity=32.4324324324324, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=31.219512195122, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28571730, Length=229, Percent_Identity=25.764192139738, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571729, Length=229, Percent_Identity=25.764192139738, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583077, Length=194, Percent_Identity=32.9896907216495, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583079, Length=194, Percent_Identity=32.9896907216495, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921000, Length=194, Percent_Identity=32.9896907216495, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921018, Length=210, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28161; Mature: 28161

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLNYEIVEKYTGIITLQRLQAANALSESLLNELNQLLDEIQQNDTIRCVIVTGAGTKA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FCAGADLKERKGMSDDQVIHAVKQIGETINRIEKLQVPVIAAINGVALGGGLELALACDI
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEECCCEEEEEEEEE
RIAVNNTKLGLTETSLAIIPGAGGTQRLPRTIGVGHAKRLIYSAVPVNTEEALRLHLIER
EEEEECCEECEEECCEEEEECCCCCHHCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
SVSQEELLDEAVQLAKRIASNGPLAVQLAKGVINNGLDTTLENGLEIEHLSYQKVIPTHD
CCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCHHHHHCCCEEEECCHHCCCCCCH
RREGLQAFAEKRKPDYQGR
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYLNYEIVEKYTGIITLQRLQAANALSESLLNELNQLLDEIQQNDTIRCVIVTGAGTKA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FCAGADLKERKGMSDDQVIHAVKQIGETINRIEKLQVPVIAAINGVALGGGLELALACDI
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEECCCEEEEEEEEE
RIAVNNTKLGLTETSLAIIPGAGGTQRLPRTIGVGHAKRLIYSAVPVNTEEALRLHLIER
EEEEECCEECEEECCEEEEECCCCCHHCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
SVSQEELLDEAVQLAKRIASNGPLAVQLAKGVINNGLDTTLENGLEIEHLSYQKVIPTHD
CCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCHHHHHCCCEEEECCHHCCCCCCH
RREGLQAFAEKRKPDYQGR
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]