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The map label for this gene is thiN [H]

Identifier: 23098967

GI number: 23098967

Start: 1556541

End: 1557191

Strand: Direct

Name: thiN [H]

Synonym: OB1512

Alternate gene names: 23098967

Gene position: 1556541-1557191 (Clockwise)

Preceding gene: 23098966

Following gene: 23098968

Centisome position: 42.87

GC content: 33.33

Gene sequence:

>651_bases
ATGAAAACCATTGGAATTATTGGCAATGGTCCGAAAAATATGCTCCCTGATCTAAACCAATACCAATATGAAGTAGATTT
TTGGATAGGGGCAGATCGAGGGGCGCTTTATTTGGCAGAATCGAACCTAAATATAGATATTGCCCTCGGTGATTTTGACT
CCGTCTCAGACGAAGAAAAACAAAAAATTAACAAACACGCCAAGAAAGTATTAACGTATTCTACCGAAAAAAACTATACT
GATCTGGAACTTGCCATTCAAATTGCTAGTGATCTGCAAGTAGATCGTATACTATTATTTGGTGTTACTGGTGGTAGACT
CGACCATGAATTAATGAATATTCAATTATTATATCGACTACTTCAAAAAAATGTTGAGGCGAAAATTATTGATCATTCTA
ATCAGATTGAGATGGTAGAATCGGGGAAGTATACAATTAATAATCAAGGCTACACCTATATATCTTTTATTTCTTTTACA
AATAAAGTAGAAGGTTTGACTCTAGAAGGTTTTAAATATCCTTTAATAGATCAAACAATTGAATGGGGATCTTCTTTATG
TATTTCAAACAAGCTTACTCAAAAAAAAGGTACTTTTTCTTTTCGGGCAGGCATACTATTACTAATAAAGAGTTGTGATA
CGATCCAGTAA

Upstream 100 bases:

>100_bases
CGACTAGTGGTGCTGATGTATTAGTAGCTGGGAGTGCTATCTTTAATCATGAAAATCGAAAACAAGCCATTGAATTGCTA
AGAGAAAAGGCGAAAGTCTA

Downstream 100 bases:

>100_bases
TCTTAGATTGCTGGATGGTAAAACAGGAGGGGTCCTTATTCATGAAATTTTATACGATTAAACTTCCTAAGTTTGTTGGT
GGATTTGTGAAGGTTGTTAT

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKTIGIIGNGPKNMLPDLNQYQYEVDFWIGADRGALYLAESNLNIDIALGDFDSVSDEEKQKINKHAKKVLTYSTEKNYT
DLELAIQIASDLQVDRILLFGVTGGRLDHELMNIQLLYRLLQKNVEAKIIDHSNQIEMVESGKYTINNQGYTYISFISFT
NKVEGLTLEGFKYPLIDQTIEWGSSLCISNKLTQKKGTFSFRAGILLLIKSCDTIQ

Sequences:

>Translated_216_residues
MKTIGIIGNGPKNMLPDLNQYQYEVDFWIGADRGALYLAESNLNIDIALGDFDSVSDEEKQKINKHAKKVLTYSTEKNYT
DLELAIQIASDLQVDRILLFGVTGGRLDHELMNIQLLYRLLQKNVEAKIIDHSNQIEMVESGKYTINNQGYTYISFISFT
NKVEGLTLEGFKYPLIDQTIEWGSSLCISNKLTQKKGTFSFRAGILLLIKSCDTIQ
>Mature_216_residues
MKTIGIIGNGPKNMLPDLNQYQYEVDFWIGADRGALYLAESNLNIDIALGDFDSVSDEEKQKINKHAKKVLTYSTEKNYT
DLELAIQIASDLQVDRILLFGVTGGRLDHELMNIQLLYRLLQKNVEAKIIDHSNQIEMVESGKYTINNQGYTYISFISFT
NKVEGLTLEGFKYPLIDQTIEWGSSLCISNKLTQKKGTFSFRAGILLLIKSCDTIQ

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 24442; Mature: 24442

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIGIIGNGPKNMLPDLNQYQYEVDFWIGADRGALYLAESNLNIDIALGDFDSVSDEEK
CCEEEEEECCCHHHCCCCCCEEEEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCHHH
QKINKHAKKVLTYSTEKNYTDLELAIQIASDLQVDRILLFGVTGGRLDHELMNIQLLYRL
HHHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
LQKNVEAKIIDHSNQIEMVESGKYTINNQGYTYISFISFTNKVEGLTLEGFKYPLIDQTI
HHCCCCEEEECCCCCEEEEECCCEEECCCCEEEEEEEEECCCCCCEEECCCCCCHHHHHH
EWGSSLCISNKLTQKKGTFSFRAGILLLIKSCDTIQ
HHCCCEEECCCCCCCCCCEEHHHCEEEEEECCCCCC
>Mature Secondary Structure
MKTIGIIGNGPKNMLPDLNQYQYEVDFWIGADRGALYLAESNLNIDIALGDFDSVSDEEK
CCEEEEEECCCHHHCCCCCCEEEEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCHHH
QKINKHAKKVLTYSTEKNYTDLELAIQIASDLQVDRILLFGVTGGRLDHELMNIQLLYRL
HHHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
LQKNVEAKIIDHSNQIEMVESGKYTINNQGYTYISFISFTNKVEGLTLEGFKYPLIDQTI
HHCCCCEEEECCCCCEEEEECCCEEECCCCEEEEEEEEECCCCCCEEECCCCCCHHHHHH
EWGSSLCISNKLTQKKGTFSFRAGILLLIKSCDTIQ
HHCCCEEECCCCCCCCCCEEHHHCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]