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The map label for this gene is pyrE

Identifier: 23098950

GI number: 23098950

Start: 1536690

End: 1537298

Strand: Direct

Name: pyrE

Synonym: OB1495

Alternate gene names: 23098950

Gene position: 1536690-1537298 (Clockwise)

Preceding gene: 23098949

Following gene: 23098952

Centisome position: 42.33

GC content: 35.14

Gene sequence:

>609_bases
ATGAATAAACAAATTACAAAGGAACTACTAGAAATTGGAGCAGTACAGATTAATTTAGATACATATTTTACTTGGACATC
AGGATTAAAATCTCCCATCTATTGTGATAATCGTTTAACAATGAGTTATCCCAAAGTAAGAAAAAATATTGCTCGTGCAT
TTATTCTAATGCTAGAACAAGATGGCTTTAAACCTGATGTGATAGCAGGTTGTGCTACAGCAGGTATATCACATGCTGCA
TGGTTATCTGATCTTTTAGGTCTACCGATGGTGTATGTAAGATCTAAACCGAAAGGACATGGGAAAGGAAATCAAATAGA
GGGTGCCAGTGTAGAAGGAAAAACGGTACTCGTAATTGAGGATTTAATCTCTACAGGTGGATCGTCTATTGAAGCTGCAA
AAGCACTACAACAAGCAGGGGCTAATATCTTAGCAGTCTATTCCATTTTTACTTATGGGCTGGATAAATCAAAGGACGCT
TTTTTACAAGCAAATATCGCTTACAATTCCATTACCGGTTTTGATGAATTAATTCAAGAACTAATTGGTTCTGGTGAGTT
ATCAGAAGAAGAAAAAAATGAATTGTTGACTTTTAGAGAAAGTTTGTAG

Upstream 100 bases:

>100_bases
GTTGGAAGTGACTTTATCGTAGTAGGTAGAGCGATTACGCAATCTGATCATCCTTATACAGCATATATGGAAGCAAAGAA
ACGATGGGAGGAAGCTTCGG

Downstream 100 bases:

>100_bases
ATATTTTAGTATCGAAATAGAAAAATGGGGCCAGGTGAGTTTTAAAACCAAGGACTCACCAGCTACTATGCCCATGTAAA
GGAAAGGTCCTTAAGTCATA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MNKQITKELLEIGAVQINLDTYFTWTSGLKSPIYCDNRLTMSYPKVRKNIARAFILMLEQDGFKPDVIAGCATAGISHAA
WLSDLLGLPMVYVRSKPKGHGKGNQIEGASVEGKTVLVIEDLISTGGSSIEAAKALQQAGANILAVYSIFTYGLDKSKDA
FLQANIAYNSITGFDELIQELIGSGELSEEEKNELLTFRESL

Sequences:

>Translated_202_residues
MNKQITKELLEIGAVQINLDTYFTWTSGLKSPIYCDNRLTMSYPKVRKNIARAFILMLEQDGFKPDVIAGCATAGISHAA
WLSDLLGLPMVYVRSKPKGHGKGNQIEGASVEGKTVLVIEDLISTGGSSIEAAKALQQAGANILAVYSIFTYGLDKSKDA
FLQANIAYNSITGFDELIQELIGSGELSEEEKNELLTFRESL
>Mature_202_residues
MNKQITKELLEIGAVQINLDTYFTWTSGLKSPIYCDNRLTMSYPKVRKNIARAFILMLEQDGFKPDVIAGCATAGISHAA
WLSDLLGLPMVYVRSKPKGHGKGNQIEGASVEGKTVLVIEDLISTGGSSIEAAKALQQAGANILAVYSIFTYGLDKSKDA
FLQANIAYNSITGFDELIQELIGSGELSEEEKNELLTFRESL

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=121, Percent_Identity=29.7520661157025, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508631, Length=177, Percent_Identity=31.638418079096, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_OCEIH (Q8ER35)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692416.1
- ProteinModelPortal:   Q8ER35
- SMR:   Q8ER35
- GeneID:   1017754
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1495
- NMPDR:   fig|221109.1.peg.1497
- HOGENOM:   HBG404341
- OMA:   HAWRKDP
- BioCyc:   OIHE221109:OB1495-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 21997; Mature: 21997

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER; PS00012 PHOSPHOPANTETHEINE ; PS00626 RCC1_2

Important sites: BINDING 94-94 BINDING 98-98 BINDING 100-100 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQITKELLEIGAVQINLDTYFTWTSGLKSPIYCDNRLTMSYPKVRKNIARAFILMLEQ
CCHHHHHHHHHHCCEEEEECEEEEECCCCCCCEEECCCEEECCHHHHHHHHHHHHHHHHC
DGFKPDVIAGCATAGISHAAWLSDLLGLPMVYVRSKPKGHGKGNQIEGASVEGKTVLVIE
CCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
DLISTGGSSIEAAKALQQAGANILAVYSIFTYGLDKSKDAFLQANIAYNSITGFDELIQE
HHHHCCCCCHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHH
LIGSGELSEEEKNELLTFRESL
HHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKQITKELLEIGAVQINLDTYFTWTSGLKSPIYCDNRLTMSYPKVRKNIARAFILMLEQ
CCHHHHHHHHHHCCEEEEECEEEEECCCCCCCEEECCCEEECCHHHHHHHHHHHHHHHHC
DGFKPDVIAGCATAGISHAAWLSDLLGLPMVYVRSKPKGHGKGNQIEGASVEGKTVLVIE
CCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
DLISTGGSSIEAAKALQQAGANILAVYSIFTYGLDKSKDAFLQANIAYNSITGFDELIQE
HHHHCCCCCHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHH
LIGSGELSEEEKNELLTFRESL
HHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376