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The map label for this gene is def

Identifier: 23098865

GI number: 23098865

Start: 1453258

End: 1453809

Strand: Reverse

Name: def

Synonym: OB1410

Alternate gene names: 23098865

Gene position: 1453809-1453258 (Counterclockwise)

Preceding gene: 23098872

Following gene: 23098864

Centisome position: 40.04

GC content: 36.23

Gene sequence:

>552_bases
ATGATTACAATGAAAGATATTGTTCGTGAGGGCCACCCTTCTTTAACAAGAAGTGCAGCAGTTGTGGAGGTTCCATTATC
AAAAGATGATAAACAATTACTAGAAGATATGATGCAATTCTTGAAAAATAGTCAGGATGAAGAAATAGCAGAGAAATACG
AATTGCGTGCAGGTGTCGGTATAGCAGCTCCCCAATTGGGTATTGAAAAGCAGATTATTGCTATCCATTTTGAAGATATA
GATGGGAAATTATATAGTATGGGATTGGTTAATCCTAAAATAATCAGCCATTCAGTAGAACAGTCTTATTTATCGAGTGG
AGAAGGATGTTTATCCGTCGATCGTCCAGTAGAAGGGTATGTCCCTCGCCATGCTAGGATTACTATCAAAGCTACCGATA
TTAATGACCAACCAGTTAAATTACGACTAAAAGGGTATCCAGCAATAGTATTTCAACATGAGATTGATCACATTAATGGA
ATTATGTTTTTCGATCGGATTAATACAGAAGATCCTTTTAACATACCTGAAAATTCCTCCCCTATTCAATAA

Upstream 100 bases:

>100_bases
GTTAAAATGATAGAGGTATTAAAATGAAGTGTATTTTCATTATCATTATATATGATTACCCAATAATAACTTAATTTACA
CATGAAAAGGAGATAGTATC

Downstream 100 bases:

>100_bases
TTAAATAAAATGAAACTAACATTCAGTGATTAAGCTTTTTCTCCACTGAATGTTAGTTAAACAGAATAGACATTCACTGG
AACTCCTCCTTCTTTTATTC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MITMKDIVREGHPSLTRSAAVVEVPLSKDDKQLLEDMMQFLKNSQDEEIAEKYELRAGVGIAAPQLGIEKQIIAIHFEDI
DGKLYSMGLVNPKIISHSVEQSYLSSGEGCLSVDRPVEGYVPRHARITIKATDINDQPVKLRLKGYPAIVFQHEIDHING
IMFFDRINTEDPFNIPENSSPIQ

Sequences:

>Translated_183_residues
MITMKDIVREGHPSLTRSAAVVEVPLSKDDKQLLEDMMQFLKNSQDEEIAEKYELRAGVGIAAPQLGIEKQIIAIHFEDI
DGKLYSMGLVNPKIISHSVEQSYLSSGEGCLSVDRPVEGYVPRHARITIKATDINDQPVKLRLKGYPAIVFQHEIDHING
IMFFDRINTEDPFNIPENSSPIQ
>Mature_183_residues
MITMKDIVREGHPSLTRSAAVVEVPLSKDDKQLLEDMMQFLKNSQDEEIAEKYELRAGVGIAAPQLGIEKQIIAIHFEDI
DGKLYSMGLVNPKIISHSVEQSYLSSGEGCLSVDRPVEGYVPRHARITIKATDINDQPVKLRLKGYPAIVFQHEIDHING
IMFFDRINTEDPFNIPENSSPIQ

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=112, Percent_Identity=36.6071428571429, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_OCEIH (Q8ER96)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692331.1
- ProteinModelPortal:   Q8ER96
- SMR:   Q8ER96
- GeneID:   1017640
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1410
- NMPDR:   fig|221109.1.peg.1416
- HOGENOM:   HBG665227
- OMA:   DIIKEGH
- ProtClustDB:   PRK00150
- BioCyc:   OIHE221109:OB1410-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20556; Mature: 20556

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMKDIVREGHPSLTRSAAVVEVPLSKDDKQLLEDMMQFLKNSQDEEIAEKYELRAGVG
CCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IAAPQLGIEKQIIAIHFEDIDGKLYSMGLVNPKIISHSVEQSYLSSGEGCLSVDRPVEGY
CCCCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCC
VPRHARITIKATDINDQPVKLRLKGYPAIVFQHEIDHINGIMFFDRINTEDPFNIPENSS
CCCCEEEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC
PIQ
CCC
>Mature Secondary Structure
MITMKDIVREGHPSLTRSAAVVEVPLSKDDKQLLEDMMQFLKNSQDEEIAEKYELRAGVG
CCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IAAPQLGIEKQIIAIHFEDIDGKLYSMGLVNPKIISHSVEQSYLSSGEGCLSVDRPVEGY
CCCCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCC
VPRHARITIKATDINDQPVKLRLKGYPAIVFQHEIDHINGIMFFDRINTEDPFNIPENSS
CCCCEEEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC
PIQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376