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The map label for this gene is rnjA [H]

Identifier: 23098863

GI number: 23098863

Start: 1450721

End: 1452388

Strand: Reverse

Name: rnjA [H]

Synonym: OB1408

Alternate gene names: 23098863

Gene position: 1452388-1450721 (Counterclockwise)

Preceding gene: 23098864

Following gene: 23098855

Centisome position: 40.0

GC content: 36.57

Gene sequence:

>1668_bases
ATGAAATTTGTCAAAAATGATCAAGCTGCTGTTTTCGCTCTAGGTGGACTAGGTGAAATCGGCAAAAATACGTATGCCAT
CCAATTTCAAGATGAAATTATTTTAATTGATGCTGGGATTAAATTTCCTGAAGATGAACTTTTAGGAATTGATTATGTTA
TCCCTGATTATACGTATTTAGTACAAAACGAAGATAAAATAAAAGGGTTAATTATTACCCATGGTCATGAAGACCACATT
GGTGGTATTCCATTTTTACTGCGTGAAATTAATGTGCCAATTTACGCAGGTCCATTAGCAATTGGACTAATTCGCAATAA
ATTAGAAGAGCATGGCTTACTAAGAAATGCAAAACTAATTACCATTCAAGAAGATGATATTATTAAATTCCGAAAAACAT
CCGTTAGCTTCTTCCGTACGACACATAGTATCCCTGATTCTTATGGCGTCGTAGTAAAAACACCACCTGGTAACATTGTT
CATACTGGTGATTTCAAATTTGATTTCACACCTGTTGGTGAACCCGCTAACCTTGCAAAAATGGCTGAGATTGGTTCAGA
AGGTGTATTATGCCTTCTGTCAGATAGTACTAATAGTGAAGTACCTGGGTTTACAATGTCCGAGCGTGTCGTTGGAGAAA
GTATTCACGATATATTCAGCAGAGTCGATGGTAGATTGATCTTCGCAACATTTGCTTCCAACATCTATCGTTTACAACAA
GTTGTTGAAGCAGCTGTTAAGTACAATCGTAAAGTAGCTGTATTTGGTAGAAGTATGGAATCTGCTATAAATATTGGCCT
GGAATTAGGTTATATCCAAGCGCCGAAAGATACATTTATTGAAGGTAACCAGATTAATCGTCTTCCTGCAAATGAAGTAA
CGATCCTCTGTACTGGTTCCCAAGGTGAAACAATGGCTGCACTATCTCGAATTGCAAATGGTACACATAGACAAATTCAA
TTAATTCCTGGAGACACTGTAGTATTCTCTTCCTCTCCAATCCCGGGGAATACTGTCAGCGTAAGCAGAACCATTAATAA
ACTTTATCGAGCAGGGGCAGATGTTGTTCATGGTAAGTTGAGTAATATTCATACCTCTGGTCATGGTAGTCAAGAAGAAC
AAAAACTAATGCTTCGCTTAATTCGTCCGAAATTTTTCATGCCAATACATGGAGAATATCGTATGCTAAAAGAACATGTT
AAATTAGGAGAATTATGTGATGTAGAAGCTGAAAATTCTTTTGTAATGGATAATGGTGATGTTCTAGCACTAAGCAAGGA
TCATGCAGCTATTGCTGGGAAAATACCTTCTGGATCCGTATACGTTGACGGTAGTGGAATTGGTGATATAGGAAATATTG
TTCTACGAGATCGTCGCATCTTATCTGAAGAAGGATTAGTTATTGTTGTAGTGAGCATTAACATGAGTGACTTCAAGATA
GCTTCTGGGCCAGATATTATCTCTCGAGGCTTTGTATATATGAGAGAATCTGAAGACTTAATAAACCAAGCTCAAAAATT
AGTGTCTAATCACTTAGAAAAAGTAATGGAGCGTAAAACAACACAATGGTCAGAGATTAAAAATGAAATCACTGATACAA
TTGCTCCATTCCTATATGAAAAAACAAAAAGACGTCCAATGGTACTTCCAATTATCATGGAGATTTAA

Upstream 100 bases:

>100_bases
TGACCATAACTATAATATTGAATTTGTTCAACCGTTGGATGAGGCACACCTCCAATATGAAAAAGACACTGGAAAATTAG
AATTGGAGAACCTGTAGCCG

Downstream 100 bases:

>100_bases
AGAACAAAGGCGCATTCGCTCGTTTAGAAGTGGAGCTAGTACGCCCCCCCTTTTCTAAAAGAAGTTCGACTCTGGTCGAA
CTTCTTTTTTATATACAGGA

Product: hypothetical protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKFVKNDQAAVFALGGLGEIGKNTYAIQFQDEIILIDAGIKFPEDELLGIDYVIPDYTYLVQNEDKIKGLIITHGHEDHI
GGIPFLLREINVPIYAGPLAIGLIRNKLEEHGLLRNAKLITIQEDDIIKFRKTSVSFFRTTHSIPDSYGVVVKTPPGNIV
HTGDFKFDFTPVGEPANLAKMAEIGSEGVLCLLSDSTNSEVPGFTMSERVVGESIHDIFSRVDGRLIFATFASNIYRLQQ
VVEAAVKYNRKVAVFGRSMESAINIGLELGYIQAPKDTFIEGNQINRLPANEVTILCTGSQGETMAALSRIANGTHRQIQ
LIPGDTVVFSSSPIPGNTVSVSRTINKLYRAGADVVHGKLSNIHTSGHGSQEEQKLMLRLIRPKFFMPIHGEYRMLKEHV
KLGELCDVEAENSFVMDNGDVLALSKDHAAIAGKIPSGSVYVDGSGIGDIGNIVLRDRRILSEEGLVIVVVSINMSDFKI
ASGPDIISRGFVYMRESEDLINQAQKLVSNHLEKVMERKTTQWSEIKNEITDTIAPFLYEKTKRRPMVLPIIMEI

Sequences:

>Translated_555_residues
MKFVKNDQAAVFALGGLGEIGKNTYAIQFQDEIILIDAGIKFPEDELLGIDYVIPDYTYLVQNEDKIKGLIITHGHEDHI
GGIPFLLREINVPIYAGPLAIGLIRNKLEEHGLLRNAKLITIQEDDIIKFRKTSVSFFRTTHSIPDSYGVVVKTPPGNIV
HTGDFKFDFTPVGEPANLAKMAEIGSEGVLCLLSDSTNSEVPGFTMSERVVGESIHDIFSRVDGRLIFATFASNIYRLQQ
VVEAAVKYNRKVAVFGRSMESAINIGLELGYIQAPKDTFIEGNQINRLPANEVTILCTGSQGETMAALSRIANGTHRQIQ
LIPGDTVVFSSSPIPGNTVSVSRTINKLYRAGADVVHGKLSNIHTSGHGSQEEQKLMLRLIRPKFFMPIHGEYRMLKEHV
KLGELCDVEAENSFVMDNGDVLALSKDHAAIAGKIPSGSVYVDGSGIGDIGNIVLRDRRILSEEGLVIVVVSINMSDFKI
ASGPDIISRGFVYMRESEDLINQAQKLVSNHLEKVMERKTTQWSEIKNEITDTIAPFLYEKTKRRPMVLPIIMEI
>Mature_555_residues
MKFVKNDQAAVFALGGLGEIGKNTYAIQFQDEIILIDAGIKFPEDELLGIDYVIPDYTYLVQNEDKIKGLIITHGHEDHI
GGIPFLLREINVPIYAGPLAIGLIRNKLEEHGLLRNAKLITIQEDDIIKFRKTSVSFFRTTHSIPDSYGVVVKTPPGNIV
HTGDFKFDFTPVGEPANLAKMAEIGSEGVLCLLSDSTNSEVPGFTMSERVVGESIHDIFSRVDGRLIFATFASNIYRLQQ
VVEAAVKYNRKVAVFGRSMESAINIGLELGYIQAPKDTFIEGNQINRLPANEVTILCTGSQGETMAALSRIANGTHRQIQ
LIPGDTVVFSSSPIPGNTVSVSRTINKLYRAGADVVHGKLSNIHTSGHGSQEEQKLMLRLIRPKFFMPIHGEYRMLKEHV
KLGELCDVEAENSFVMDNGDVLALSKDHAAIAGKIPSGSVYVDGSGIGDIGNIVLRDRRILSEEGLVIVVVSINMSDFKI
ASGPDIISRGFVYMRESEDLINQAQKLVSNHLEKVMERKTTQWSEIKNEITDTIAPFLYEKTKRRPMVLPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61334; Mature: 61334

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVKNDQAAVFALGGLGEIGKNTYAIQFQDEIILIDAGIKFPEDELLGIDYVIPDYTYL
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEEEECCCCCCCHHHEEEEEECCCEEEE
VQNEDKIKGLIITHGHEDHIGGIPFLLREINVPIYAGPLAIGLIRNKLEEHGLLRNAKLI
EECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCEEE
TIQEDDIIKFRKTSVSFFRTTHSIPDSYGVVVKTPPGNIVHTGDFKFDFTPVGEPANLAK
EEECCCCEEEECCCCEEHHHHCCCCCCCCEEEECCCCCEEECCCCEEEEECCCCCHHHHH
MAEIGSEGVLCLLSDSTNSEVPGFTMSERVVGESIHDIFSRVDGRLIFATFASNIYRLQQ
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
VVEAAVKYNRKVAVFGRSMESAINIGLELGYIQAPKDTFIEGNQINRLPANEVTILCTGS
HHHHHHHCCCEEEEECCCHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
QGETMAALSRIANGTHRQIQLIPGDTVVFSSSPIPGNTVSVSRTINKLYRAGADVVHGKL
CCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCEEEHHHHHHHHHHCCCHHEECHH
SNIHTSGHGSQEEQKLMLRLIRPKFFMPIHGEYRMLKEHVKLGELCDVEAENSFVMDNGD
HCEEECCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEECCCC
VLALSKDHAAIAGKIPSGSVYVDGSGIGDIGNIVLRDRRILSEEGLVIVVVSINMSDFKI
EEEEECCCCEEEECCCCCCEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEECCCCEEE
ASGPDIISRGFVYMRESEDLINQAQKLVSNHLEKVMERKTTQWSEIKNEITDTIAPFLYE
CCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KTKRRPMVLPIIMEI
HHCCCCEEEEEEEEC
>Mature Secondary Structure
MKFVKNDQAAVFALGGLGEIGKNTYAIQFQDEIILIDAGIKFPEDELLGIDYVIPDYTYL
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEEEECCCCCCCHHHEEEEEECCCEEEE
VQNEDKIKGLIITHGHEDHIGGIPFLLREINVPIYAGPLAIGLIRNKLEEHGLLRNAKLI
EECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCCEEE
TIQEDDIIKFRKTSVSFFRTTHSIPDSYGVVVKTPPGNIVHTGDFKFDFTPVGEPANLAK
EEECCCCEEEECCCCEEHHHHCCCCCCCCEEEECCCCCEEECCCCEEEEECCCCCHHHHH
MAEIGSEGVLCLLSDSTNSEVPGFTMSERVVGESIHDIFSRVDGRLIFATFASNIYRLQQ
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
VVEAAVKYNRKVAVFGRSMESAINIGLELGYIQAPKDTFIEGNQINRLPANEVTILCTGS
HHHHHHHCCCEEEEECCCHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
QGETMAALSRIANGTHRQIQLIPGDTVVFSSSPIPGNTVSVSRTINKLYRAGADVVHGKL
CCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCEEEHHHHHHHHHHCCCHHEECHH
SNIHTSGHGSQEEQKLMLRLIRPKFFMPIHGEYRMLKEHVKLGELCDVEAENSFVMDNGD
HCEEECCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEECCCC
VLALSKDHAAIAGKIPSGSVYVDGSGIGDIGNIVLRDRRILSEEGLVIVVVSINMSDFKI
EEEEECCCCEEEECCCCCCEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEECCCCEEE
ASGPDIISRGFVYMRESEDLINQAQKLVSNHLEKVMERKTTQWSEIKNEITDTIAPFLYE
CCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KTKRRPMVLPIIMEI
HHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]