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The map label for this gene is nfrA1 [H]

Identifier: 23098840

GI number: 23098840

Start: 1430921

End: 1431670

Strand: Reverse

Name: nfrA1 [H]

Synonym: OB1385

Alternate gene names: 23098840

Gene position: 1431670-1430921 (Counterclockwise)

Preceding gene: 23098841

Following gene: 23098838

Centisome position: 39.43

GC content: 32.13

Gene sequence:

>750_bases
ATGAATAAAATTATTGAAACACTTAAAAATCACCGATCAATTAGAAAATTTACGAATCAACCTCTAACCGATTATCAAAT
AAAGGAAATTATAGAATCAGCTCAATGTGCATCTACATCGAGTAATGTCATGGCATATACAATTATCGGTGTTACCGATA
CCACATTAAAGCAAGATCTGTATGAAATCTCTGGACATAATCATGTAAAAACAAGTGCACATTTATTACTATTTTGTGCA
GATTTACATCGAATACATCAATTAGAAGATAATCCCGATGAAAATCCTGATTACCTCAATAATTTGGAAAGCACCGAGCA
ATTTATGGTATCTGTTATTGATGCTACCCTAGCTTCACAAAATGCAGCAATAGCTGCTGAATCAATGGGATTGGGAATTT
GTTATTTAGGTAGTTTACGTAATAACATACGTAAATTTAATGATTTATTGCATTTACCTCAACATGTTATCCCGTTATTT
GGTTTAGCAATTGGACACCCTGATCATCAACCCGATATCAAACCGCGCTTACCAATGGAAACTATCTATCATGAGAATAC
ATATAAACCATTTGAGGATCAAAAAAGTTACATACAAAAATATGATGAAACTGTATCGGATTATTATGCAAGCCGTTCTT
CAAATAATAAAATTGATACCTGGTCCAATCAAATGAAAAGAAAATTCGCAATTCCAACACGTATGGATGTTAGTTCTTTT
GTTCAGGAAAAACAGTTGAATAGAAAATAA

Upstream 100 bases:

>100_bases
GACCAATCATACCGTTCTTGGGATGAATCTGAAGAAAAAAAGCACGAAAATCACACCATGATAATATCAAAGAATATCAA
TGACTAGGAGGTATAAGTGT

Downstream 100 bases:

>100_bases
ATCGTTCTTCTCTATACCATGGAGATAATTTCACTCTTATCCCATAATAAATGCCAACCCAATACAAAATAACAAAAGAC
CGAGTTGTTTAAATAATTAA

Product: NADPH-flavin oxidoreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNKIIETLKNHRSIRKFTNQPLTDYQIKEIIESAQCASTSSNVMAYTIIGVTDTTLKQDLYEISGHNHVKTSAHLLLFCA
DLHRIHQLEDNPDENPDYLNNLESTEQFMVSVIDATLASQNAAIAAESMGLGICYLGSLRNNIRKFNDLLHLPQHVIPLF
GLAIGHPDHQPDIKPRLPMETIYHENTYKPFEDQKSYIQKYDETVSDYYASRSSNNKIDTWSNQMKRKFAIPTRMDVSSF
VQEKQLNRK

Sequences:

>Translated_249_residues
MNKIIETLKNHRSIRKFTNQPLTDYQIKEIIESAQCASTSSNVMAYTIIGVTDTTLKQDLYEISGHNHVKTSAHLLLFCA
DLHRIHQLEDNPDENPDYLNNLESTEQFMVSVIDATLASQNAAIAAESMGLGICYLGSLRNNIRKFNDLLHLPQHVIPLF
GLAIGHPDHQPDIKPRLPMETIYHENTYKPFEDQKSYIQKYDETVSDYYASRSSNNKIDTWSNQMKRKFAIPTRMDVSSF
VQEKQLNRK
>Mature_249_residues
MNKIIETLKNHRSIRKFTNQPLTDYQIKEIIESAQCASTSSNVMAYTIIGVTDTTLKQDLYEISGHNHVKTSAHLLLFCA
DLHRIHQLEDNPDENPDYLNNLESTEQFMVSVIDATLASQNAAIAAESMGLGICYLGSLRNNIRKFNDLLHLPQHVIPLF
GLAIGHPDHQPDIKPRLPMETIYHENTYKPFEDQKSYIQKYDETVSDYYASRSSNNKIDTWSNQMKRKFAIPTRMDVSSF
VQEKQLNRK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=235, Percent_Identity=43.4042553191489, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 28603; Mature: 28603

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIIETLKNHRSIRKFTNQPLTDYQIKEIIESAQCASTSSNVMAYTIIGVTDTTLKQDL
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHH
YEISGHNHVKTSAHLLLFCADLHRIHQLEDNPDENPDYLNNLESTEQFMVSVIDATLASQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NAAIAAESMGLGICYLGSLRNNIRKFNDLLHLPQHVIPLFGLAIGHPDHQPDIKPRLPME
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
TIYHENTYKPFEDQKSYIQKYDETVSDYYASRSSNNKIDTWSNQMKRKFAIPTRMDVSSF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
VQEKQLNRK
HHHHHHCCC
>Mature Secondary Structure
MNKIIETLKNHRSIRKFTNQPLTDYQIKEIIESAQCASTSSNVMAYTIIGVTDTTLKQDL
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHH
YEISGHNHVKTSAHLLLFCADLHRIHQLEDNPDENPDYLNNLESTEQFMVSVIDATLASQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NAAIAAESMGLGICYLGSLRNNIRKFNDLLHLPQHVIPLFGLAIGHPDHQPDIKPRLPME
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
TIYHENTYKPFEDQKSYIQKYDETVSDYYASRSSNNKIDTWSNQMKRKFAIPTRMDVSSF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
VQEKQLNRK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]