Click here to switch to the map view.

The map label for this gene is cotA [H]

Identifier: 23098801

GI number: 23098801

Start: 1388773

End: 1390314

Strand: Reverse

Name: cotA [H]

Synonym: OB1346

Alternate gene names: 23098801

Gene position: 1390314-1388773 (Counterclockwise)

Preceding gene: 23098802

Following gene: 23098796

Centisome position: 38.3

GC content: 37.55

Gene sequence:

>1542_bases
ATGAAATTAACTAAATTTGTAGATGAGCTTCCTATTATCCAAACTTTACATCCAACAGAAAAAAGCAATCATTTAACATA
TTATGAAGTGACTATGAAAGAAGGTTGGCATAAGCTTCATCGTGATTTACCATCAACAAAGCTATGGGGATATAATGGGC
AATTTCCAGGACCTACCATAGAAGTTTCTGAAAGGGAAATGATACATGTTAAATGGATGAACCATTTACCACCGAAACTA
ATGTTACCTTTAGATACTTCGATACATCATCTGGACCAAATGCCGCAAACTAGAACGGTCACGCATGTTCATGGCAGTGT
AACAAAACCAGATAGTGACGGTTATCCTGAGGCTTGGTTTACCCGCGGATTTCAAGAGACTGGTCCTGATTTTTCTCGAG
AAGTATATGAGTATCCAAACAATCAACGTGCTGCTACGCTATGGTATCATGATCATGCCATGGGAATTACTCGTTTAAAC
GTATATGCTGGCCTTGTTGGCATGTATATTATTCGTGGTGAAGAGGAAAAAGCCTTACAGCTACCATCCGGTGACTACGA
AATACCCTTAATTATCTGTGACCGAACACTTAATCCAGATGGTTCATTATTCTATCCTTCTGGACCTGATGAACCAATCC
CAGGAGCACCCTCCCCTTCTATTGTCCCTGCATATTTAGGGGAAACAATACTCGTTAATGGGAAGGCTTGGCCATATATG
GATGTCGAACCAAGAAAATATCGATTTAGACTAGTAAATGCCTCTAATACTCGAACATATCGGATATCCTTGAATAATGA
CGTACCGATATATCAAATTGGTTCTGATGGTGGACTACTACGTAAAAGTATTCCTACCCGACAATTTGTTATAGAACCAG
CAGAACGTATAGATATTGTCATTGACTTTAGCGAATATAAAGGGCAGGTTATAAATATAAACAATGATTTAGGTGAGGAT
GCCGATCCAGATGATCAAACCAATCATATTATGCAATTTAAAGTCTACAAACCTTTAAAGAATAAAGATATAAGCAGAAT
TCCCAAACACTTAACGCATATACCCTCACTAAAACAAAATTCAATAAATACTATTCGAAATCTAAAACTAGTGGGATCAG
AAGATAAATTCGGAAGACCGCTACTATTACTCAATAATCAATTATGGCATGATCCAATTACTGAAAAACCTCAGCTCGGT
GATACAGAAATTTGGTCGATTGTAAACGTAACTAACTTTACACATCCCATTCATCTTCACCTTGTACAATTTCAAGTTCT
TGACCGACAACCATTCGATTTAGAAAAATATAATGAAGATGGATCAATAATTTACACGGATGCTCCACTATCACCTGCTG
AAAATGAAAAAAGTTGGAAAGATACGTTAGCAGCACCATCCGCTCACGTAACGCGCGTGATTGCCAAGTTTGAACCTTTT
ACAGGAGATTATGTGTGGCACTGTCATATACTAGAACATGAAGATTACGATATGATGCGGCCATTTACGATTGTAGATAA
AGAAGAGGAGGAAACTTTATAA

Upstream 100 bases:

>100_bases
TTCCTCTAAAAACAGATTTACGATTAATAGGTAAAAACCCAAACCACAAACACCCAATAAACATAAATGATAGTAATATG
TTTTTTTGGGAGTGAAATAA

Downstream 100 bases:

>100_bases
TTTGAGACTTCCATCAGCGGGGGATTCATTTATCCTCAGCTGATGGTTAAAGATAAATCTATCGTGCACCTATTCTGTTT
CATTATTGATACTTTTACGT

Product: spore outer coat protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKLTKFVDELPIIQTLHPTEKSNHLTYYEVTMKEGWHKLHRDLPSTKLWGYNGQFPGPTIEVSEREMIHVKWMNHLPPKL
MLPLDTSIHHLDQMPQTRTVTHVHGSVTKPDSDGYPEAWFTRGFQETGPDFSREVYEYPNNQRAATLWYHDHAMGITRLN
VYAGLVGMYIIRGEEEKALQLPSGDYEIPLIICDRTLNPDGSLFYPSGPDEPIPGAPSPSIVPAYLGETILVNGKAWPYM
DVEPRKYRFRLVNASNTRTYRISLNNDVPIYQIGSDGGLLRKSIPTRQFVIEPAERIDIVIDFSEYKGQVININNDLGED
ADPDDQTNHIMQFKVYKPLKNKDISRIPKHLTHIPSLKQNSINTIRNLKLVGSEDKFGRPLLLLNNQLWHDPITEKPQLG
DTEIWSIVNVTNFTHPIHLHLVQFQVLDRQPFDLEKYNEDGSIIYTDAPLSPAENEKSWKDTLAAPSAHVTRVIAKFEPF
TGDYVWHCHILEHEDYDMMRPFTIVDKEEEETL

Sequences:

>Translated_513_residues
MKLTKFVDELPIIQTLHPTEKSNHLTYYEVTMKEGWHKLHRDLPSTKLWGYNGQFPGPTIEVSEREMIHVKWMNHLPPKL
MLPLDTSIHHLDQMPQTRTVTHVHGSVTKPDSDGYPEAWFTRGFQETGPDFSREVYEYPNNQRAATLWYHDHAMGITRLN
VYAGLVGMYIIRGEEEKALQLPSGDYEIPLIICDRTLNPDGSLFYPSGPDEPIPGAPSPSIVPAYLGETILVNGKAWPYM
DVEPRKYRFRLVNASNTRTYRISLNNDVPIYQIGSDGGLLRKSIPTRQFVIEPAERIDIVIDFSEYKGQVININNDLGED
ADPDDQTNHIMQFKVYKPLKNKDISRIPKHLTHIPSLKQNSINTIRNLKLVGSEDKFGRPLLLLNNQLWHDPITEKPQLG
DTEIWSIVNVTNFTHPIHLHLVQFQVLDRQPFDLEKYNEDGSIIYTDAPLSPAENEKSWKDTLAAPSAHVTRVIAKFEPF
TGDYVWHCHILEHEDYDMMRPFTIVDKEEEETL
>Mature_513_residues
MKLTKFVDELPIIQTLHPTEKSNHLTYYEVTMKEGWHKLHRDLPSTKLWGYNGQFPGPTIEVSEREMIHVKWMNHLPPKL
MLPLDTSIHHLDQMPQTRTVTHVHGSVTKPDSDGYPEAWFTRGFQETGPDFSREVYEYPNNQRAATLWYHDHAMGITRLN
VYAGLVGMYIIRGEEEKALQLPSGDYEIPLIICDRTLNPDGSLFYPSGPDEPIPGAPSPSIVPAYLGETILVNGKAWPYM
DVEPRKYRFRLVNASNTRTYRISLNNDVPIYQIGSDGGLLRKSIPTRQFVIEPAERIDIVIDFSEYKGQVININNDLGED
ADPDDQTNHIMQFKVYKPLKNKDISRIPKHLTHIPSLKQNSINTIRNLKLVGSEDKFGRPLLLLNNQLWHDPITEKPQLG
DTEIWSIVNVTNFTHPIHLHLVQFQVLDRQPFDLEKYNEDGSIIYTDAPLSPAENEKSWKDTLAAPSAHVTRVIAKFEPF
TGDYVWHCHILEHEDYDMMRPFTIVDKEEEETL

Specific function: Involved in brown pigmentation during sporogenesis [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To S.antibioticus phenoxazinone synthase (phsA) [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=509, Percent_Identity=24.950884086444, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789394, Length=435, Percent_Identity=26.6666666666667, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR008972 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 59090; Mature: 59090

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKFVDELPIIQTLHPTEKSNHLTYYEVTMKEGWHKLHRDLPSTKLWGYNGQFPGPTI
CCHHHHHHHCCCEEEECCCCCCCCEEEEEEEHHHHHHHHHHCCCCCEECCCCCCCCCCEE
EVSEREMIHVKWMNHLPPKLMLPLDTSIHHLDQMPQTRTVTHVHGSVTKPDSDGYPEAWF
EECCCEEEEEEEHHCCCCEEEEECCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHH
TRGFQETGPDFSREVYEYPNNQRAATLWYHDHAMGITRLNVYAGLVGMYIIRGEEEKALQ
HCCHHHCCCCHHHHHHHCCCCCEEEEEEEECCCCCEEEHHHHHHEEEEEEEECCCCCEEE
LPSGDYEIPLIICDRTLNPDGSLFYPSGPDEPIPGAPSPSIVPAYLGETILVNGKAWPYM
CCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEHHHCCCEEEECCCCCCEE
DVEPRKYRFRLVNASNTRTYRISLNNDVPIYQIGSDGGLLRKSIPTRQFVIEPAERIDIV
CCCCCEEEEEEEECCCCEEEEEEECCCCCEEEECCCCCEEHHCCCCCEEEECCCHHEEEE
IDFSEYKGQVININNDLGEDADPDDQTNHIMQFKVYKPLKNKDISRIPKHLTHIPSLKQN
EEEHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCC
SINTIRNLKLVGSEDKFGRPLLLLNNQLWHDPITEKPQLGDTEIWSIVNVTNFTHPIHLH
CHHHHHEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LVQFQVLDRQPFDLEKYNEDGSIIYTDAPLSPAENEKSWKDTLAAPSAHVTRVIAKFEPF
EEEEEEECCCCCCHHHCCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCC
TGDYVWHCHILEHEDYDMMRPFTIVDKEEEETL
CCCEEEEEEEEECCCCCCCCCEEEECCCHHCCC
>Mature Secondary Structure
MKLTKFVDELPIIQTLHPTEKSNHLTYYEVTMKEGWHKLHRDLPSTKLWGYNGQFPGPTI
CCHHHHHHHCCCEEEECCCCCCCCEEEEEEEHHHHHHHHHHCCCCCEECCCCCCCCCCEE
EVSEREMIHVKWMNHLPPKLMLPLDTSIHHLDQMPQTRTVTHVHGSVTKPDSDGYPEAWF
EECCCEEEEEEEHHCCCCEEEEECCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHH
TRGFQETGPDFSREVYEYPNNQRAATLWYHDHAMGITRLNVYAGLVGMYIIRGEEEKALQ
HCCHHHCCCCHHHHHHHCCCCCEEEEEEEECCCCCEEEHHHHHHEEEEEEEECCCCCEEE
LPSGDYEIPLIICDRTLNPDGSLFYPSGPDEPIPGAPSPSIVPAYLGETILVNGKAWPYM
CCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEHHHCCCEEEECCCCCCEE
DVEPRKYRFRLVNASNTRTYRISLNNDVPIYQIGSDGGLLRKSIPTRQFVIEPAERIDIV
CCCCCEEEEEEEECCCCEEEEEEECCCCCEEEECCCCCEEHHCCCCCEEEECCCHHEEEE
IDFSEYKGQVININNDLGEDADPDDQTNHIMQFKVYKPLKNKDISRIPKHLTHIPSLKQN
EEEHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCC
SINTIRNLKLVGSEDKFGRPLLLLNNQLWHDPITEKPQLGDTEIWSIVNVTNFTHPIHLH
CHHHHHEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LVQFQVLDRQPFDLEKYNEDGSIIYTDAPLSPAENEKSWKDTLAAPSAHVTRVIAKFEPF
EEEEEEECCCCCCHHHCCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCC
TGDYVWHCHILEHEDYDMMRPFTIVDKEEEETL
CCCEEEEEEEEECCCCCCCCCEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969499; 9455482; 9384377; 2821284; 3135411 [H]