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The map label for this gene is trpS

Identifier: 23098663

GI number: 23098663

Start: 1256619

End: 1257602

Strand: Reverse

Name: trpS

Synonym: OB1208

Alternate gene names: 23098663

Gene position: 1257602-1256619 (Counterclockwise)

Preceding gene: 23098666

Following gene: 23098656

Centisome position: 34.64

GC content: 34.76

Gene sequence:

>984_bases
ATGAAAACAATTTTTTCTGGTATACAACCAAGTGGTACATTAACACTTGGAAACTATTTAGGAGCAATCCAACAATTTGT
GGAATTACAAAACGATTATAATTGCTATTTCTGTATTGTGGATGAACATGCTATAACCGTACCACAAGATCGACTTGAGT
TACGTAAGAATATTAAATCATTAGCAGCATTATACATTGCATCAGGAATCGATCCAGATTCTTCTACACTTTTTATTCAA
TCTGAAGTACCAGAACATACTCAATTAGGATGGATGTTACAATCAATAAGTTATGTTGGTGAATTGGAGAGAATGACTCA
ATATAAAGATAAATCGCAAGGTCAAGAAGCGATTTCTTCTGCTCTTTTAACTTATCCTTCGCTTATGGCAGCTGATATCT
TACTCTATAATACGGATGTTGTCCCTGTTGGAGATGATCAAAAACAGCATTTAGAATTAGCTCGAAATCTTGCGCAACGT
TTTAATAATCGATTCAATGATATCTTTACTGTACCTGAAGTACGAATTCCCAAAGTCGGTGCACGGATTATGTCTCTTCA
AGAGCCTACTAAAAAAATGAGTAAATCCGATACAAATCAAAAAGGTTTTATCTCTATGCTTGATGAACCTAAACGAATTG
AGAAAAAGATTAAAAGCGCTGTTACTGATTCAGAAGGAATTGTAAAATTTGATAAGGAAAATAAACCTGGAGTATCGAAC
CTACTGACCATATATTCTAGTTGTACTGGTGAATCTATCGCTGATCTAGAAAAGAAATATGATGGTAAAGGCTATGGTGA
TTTCAAACAAGGCGTAGCAAATGCTGTAATTGATACACTTCGCCCTATTCAGGAGAAATATGAGCAATTAATCCAATCAG
ATGAATTAGACGCCATCCTTGATCAAGGTAGAGATAAGGCATCTTTCTCGGCTGGAAAAACAATAAAGAAAGCCAAAAAA
GCCATGGGTTTAGGAAGAAAATAA

Upstream 100 bases:

>100_bases
GTACAATGCAAGATAATAAGGGTGGCAACGCGGTTCATTCGTCCCTAGATATGGGGAGAATGGGCTTTTTTTAATGAATA
AATTTATTGAGGTGATTGAA

Downstream 100 bases:

>100_bases
ATCCGCATAAACAAAATAGGTTTCCTTGTTCTCACAATCGCGAACAAGGAAACCCATTTTTTATTTTTGATTGGAAGTAT
GCTGTTCATTACCCATTTCC

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKTIFSGIQPSGTLTLGNYLGAIQQFVELQNDYNCYFCIVDEHAITVPQDRLELRKNIKSLAALYIASGIDPDSSTLFIQ
SEVPEHTQLGWMLQSISYVGELERMTQYKDKSQGQEAISSALLTYPSLMAADILLYNTDVVPVGDDQKQHLELARNLAQR
FNNRFNDIFTVPEVRIPKVGARIMSLQEPTKKMSKSDTNQKGFISMLDEPKRIEKKIKSAVTDSEGIVKFDKENKPGVSN
LLTIYSSCTGESIADLEKKYDGKGYGDFKQGVANAVIDTLRPIQEKYEQLIQSDELDAILDQGRDKASFSAGKTIKKAKK
AMGLGRK

Sequences:

>Translated_327_residues
MKTIFSGIQPSGTLTLGNYLGAIQQFVELQNDYNCYFCIVDEHAITVPQDRLELRKNIKSLAALYIASGIDPDSSTLFIQ
SEVPEHTQLGWMLQSISYVGELERMTQYKDKSQGQEAISSALLTYPSLMAADILLYNTDVVPVGDDQKQHLELARNLAQR
FNNRFNDIFTVPEVRIPKVGARIMSLQEPTKKMSKSDTNQKGFISMLDEPKRIEKKIKSAVTDSEGIVKFDKENKPGVSN
LLTIYSSCTGESIADLEKKYDGKGYGDFKQGVANAVIDTLRPIQEKYEQLIQSDELDAILDQGRDKASFSAGKTIKKAKK
AMGLGRK
>Mature_327_residues
MKTIFSGIQPSGTLTLGNYLGAIQQFVELQNDYNCYFCIVDEHAITVPQDRLELRKNIKSLAALYIASGIDPDSSTLFIQ
SEVPEHTQLGWMLQSISYVGELERMTQYKDKSQGQEAISSALLTYPSLMAADILLYNTDVVPVGDDQKQHLELARNLAQR
FNNRFNDIFTVPEVRIPKVGARIMSLQEPTKKMSKSDTNQKGFISMLDEPKRIEKKIKSAVTDSEGIVKFDKENKPGVSN
LLTIYSSCTGESIADLEKKYDGKGYGDFKQGVANAVIDTLRPIQEKYEQLIQSDELDAILDQGRDKASFSAGKTIKKAKK
AMGLGRK

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=326, Percent_Identity=41.7177914110429, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI41352700, Length=174, Percent_Identity=51.1494252873563, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI1789786, Length=329, Percent_Identity=55.9270516717325, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71982800, Length=328, Percent_Identity=38.719512195122, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI71982793, Length=328, Percent_Identity=38.719512195122, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6320474, Length=344, Percent_Identity=38.6627906976744, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24666151, Length=327, Percent_Identity=41.5902140672783, Blast_Score=263, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_OCEIH (Q8ERU2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692129.1
- ProteinModelPortal:   Q8ERU2
- SMR:   Q8ERU2
- GeneID:   1017380
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1208
- NMPDR:   fig|221109.1.peg.1207
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- ProtClustDB:   PRK00927
- BioCyc:   OIHE221109:OB1208-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 36494; Mature: 36494

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIFSGIQPSGTLTLGNYLGAIQQFVELQNDYNCYFCIVDEHAITVPQDRLELRKNIKS
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHHHHHHHHH
LAALYIASGIDPDSSTLFIQSEVPEHTQLGWMLQSISYVGELERMTQYKDKSQGQEAISS
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLTYPSLMAADILLYNTDVVPVGDDQKQHLELARNLAQRFNNRFNDIFTVPEVRIPKVG
HHHHHHHHHHHHHHEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHHH
ARIMSLQEPTKKMSKSDTNQKGFISMLDEPKRIEKKIKSAVTDSEGIVKFDKENKPGVSN
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHH
LLTIYSSCTGESIADLEKKYDGKGYGDFKQGVANAVIDTLRPIQEKYEQLIQSDELDAIL
HHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DQGRDKASFSAGKTIKKAKKAMGLGRK
HCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTIFSGIQPSGTLTLGNYLGAIQQFVELQNDYNCYFCIVDEHAITVPQDRLELRKNIKS
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHHHHHHHHH
LAALYIASGIDPDSSTLFIQSEVPEHTQLGWMLQSISYVGELERMTQYKDKSQGQEAISS
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLTYPSLMAADILLYNTDVVPVGDDQKQHLELARNLAQRFNNRFNDIFTVPEVRIPKVG
HHHHHHHHHHHHHHEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHHH
ARIMSLQEPTKKMSKSDTNQKGFISMLDEPKRIEKKIKSAVTDSEGIVKFDKENKPGVSN
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHH
LLTIYSSCTGESIADLEKKYDGKGYGDFKQGVANAVIDTLRPIQEKYEQLIQSDELDAIL
HHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DQGRDKASFSAGKTIKKAKKAMGLGRK
HCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376