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The map label for this gene is prsA

Identifier: 23098603

GI number: 23098603

Start: 1200620

End: 1201519

Strand: Reverse

Name: prsA

Synonym: OB1148

Alternate gene names: 23098603

Gene position: 1201519-1200620 (Counterclockwise)

Preceding gene: 23098604

Following gene: 23098595

Centisome position: 33.09

GC content: 36.11

Gene sequence:

>900_bases
ATGAAGAAATGGACCATAGCAGCGTCACTTTCAATCGGCGTACTCGCATTATCTGCATGTAATTCAGACGATGAAATCGT
TGCTGAAACGGAAGCAGGAAATATTACAAAAGAAGAGTTTTACGAAGAATTAAAAGATGTGAATGGCGAATCCACATTGC
AACAACTTGTAACTGTAAAAGTTTTAGAAGACAATTATGAAGTAACTGACGAAGAATTAGAAGAAGAGATCTCCACAATG
AAGGAAGGATTTCCTAGTGAGGAAGATTTCAATACGACGGTAGAGCAACAATTTGGCGGTGAGGAACAACTTCGTGAAAT
AATGTACGTTAGTATGTTACAAGAAAAAGCAGCTGCTGAAGACGTTGAAATTACGGAAGAAGATTTACAAGAGTTATATG
AACGTAAAAATACAGAAATTCAAGCACAACATATTCTTTTAGAAAATGAAGAAGATGTTGCTGAAGTTCAACAAAAAATT
GAAGACGGTGAAGACTTCGGTGAACTAGCACAAGAATATTCTACTGACACAGGATCCGCAGAGAATGGTGGAGACCTTGG
GTACTTTAGTGCCGGAAGTATGGTTCCAGAATTTGAAGAAGCAGCATTCTCATTAGAAGCAGGAGAAATTAGCGATCCAG
TGCAGAGTACTCATGGAACACATATTATTAAAGTAAATGATGTACGTGAAAAAGAAGAATCAATCGGTGAATTTGAAGAT
GTAAAAAAAGAACTTGAGCGTGAAATCTTACTTAATCGTGTAGATCAAACACAAATCCAGGAAAAAATAAATAAATTAAT
CCAAGATGCTGGTGTTCAAATTAATGTAGAAGGCATGGAAGATTTATTTGAAGCAGAAGAAACGGAAGAAAATACAACTG
AAGAAGATGCACAAGGTTAA

Upstream 100 bases:

>100_bases
GGTATTTTTACAAATTCTATTTTTTTCCTGAAAAAGTATGATATATTAATGATTGTAAAAACTAATCGCAAAATAGTACA
GAGTAGGAGTGTTTTGAAGT

Downstream 100 bases:

>100_bases
AGAACAAAAGCGCAAGCGCTCGTTTAGAAACGTAGCGAGTGGAGCGAATCAACTGAGATAAAGGAATCACGGTGAGGCGA
CAAACCGATGATGACTTTCG

Product: post-translocation molecular chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKKWTIAASLSIGVLALSACNSDDEIVAETEAGNITKEEFYEELKDVNGESTLQQLVTVKVLEDNYEVTDEELEEEISTM
KEGFPSEEDFNTTVEQQFGGEEQLREIMYVSMLQEKAAAEDVEITEEDLQELYERKNTEIQAQHILLENEEDVAEVQQKI
EDGEDFGELAQEYSTDTGSAENGGDLGYFSAGSMVPEFEEAAFSLEAGEISDPVQSTHGTHIIKVNDVREKEESIGEFED
VKKELEREILLNRVDQTQIQEKINKLIQDAGVQINVEGMEDLFEAEETEENTTEEDAQG

Sequences:

>Translated_299_residues
MKKWTIAASLSIGVLALSACNSDDEIVAETEAGNITKEEFYEELKDVNGESTLQQLVTVKVLEDNYEVTDEELEEEISTM
KEGFPSEEDFNTTVEQQFGGEEQLREIMYVSMLQEKAAAEDVEITEEDLQELYERKNTEIQAQHILLENEEDVAEVQQKI
EDGEDFGELAQEYSTDTGSAENGGDLGYFSAGSMVPEFEEAAFSLEAGEISDPVQSTHGTHIIKVNDVREKEESIGEFED
VKKELEREILLNRVDQTQIQEKINKLIQDAGVQINVEGMEDLFEAEETEENTTEEDAQG
>Mature_299_residues
MKKWTIAASLSIGVLALSACNSDDEIVAETEAGNITKEEFYEELKDVNGESTLQQLVTVKVLEDNYEVTDEELEEEISTM
KEGFPSEEDFNTTVEQQFGGEEQLREIMYVSMLQEKAAAEDVEITEEDLQELYERKNTEIQAQHILLENEEDVAEVQQKI
EDGEDFGELAQEYSTDTGSAENGGDLGYFSAGSMVPEFEEAAFSLEAGEISDPVQSTHGTHIIKVNDVREKEESIGEFED
VKKELEREILLNRVDQTQIQEKINKLIQDAGVQINVEGMEDLFEAEETEENTTEEDAQG

Specific function: Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain

Homologues:

Organism=Homo sapiens, GI38679892, Length=92, Percent_Identity=39.1304347826087, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786238, Length=126, Percent_Identity=32.5396825396825, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790211, Length=84, Percent_Identity=41.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537235, Length=95, Percent_Identity=36.8421052631579, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322478, Length=115, Percent_Identity=36.5217391304348, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356303, Length=114, Percent_Identity=34.2105263157895, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PRSA_OCEIH (Q8CXK4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692069.1
- ProteinModelPortal:   Q8CXK4
- SMR:   Q8CXK4
- GeneID:   1017274
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1148
- NMPDR:   fig|221109.1.peg.1149
- HOGENOM:   HBG712792
- OMA:   KDRDIKK
- BioCyc:   OIHE221109:OB1148-MONOMER
- BRENDA:   5.2.1.8
- HAMAP:   MF_01145
- InterPro:   IPR023059
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880

Pfam domain/function: PF00639 Rotamase; SSF109998 Trigger_fac_C_bac

EC number: =5.2.1.8

Molecular weight: Translated: 33619; Mature: 33619

Theoretical pI: Translated: 3.65; Mature: 3.65

Prosite motif: PS01096 PPIC_PPIASE_1; PS50198 PPIC_PPIASE_2; PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWTIAASLSIGVLALSACNSDDEIVAETEAGNITKEEFYEELKDVNGESTLQQLVTVK
CCCEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VLEDNYEVTDEELEEEISTMKEGFPSEEDFNTTVEQQFGGEEQLREIMYVSMLQEKAAAE
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DVEITEEDLQELYERKNTEIQAQHILLENEEDVAEVQQKIEDGEDFGELAQEYSTDTGSA
CCCCCHHHHHHHHHHCCCCEEHHHHEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
ENGGDLGYFSAGSMVPEFEEAAFSLEAGEISDPVQSTHGTHIIKVNDVREKEESIGEFED
CCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHCHHHH
VKKELEREILLNRVDQTQIQEKINKLIQDAGVQINVEGMEDLFEAEETEENTTEEDAQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKWTIAASLSIGVLALSACNSDDEIVAETEAGNITKEEFYEELKDVNGESTLQQLVTVK
CCCEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VLEDNYEVTDEELEEEISTMKEGFPSEEDFNTTVEQQFGGEEQLREIMYVSMLQEKAAAE
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DVEITEEDLQELYERKNTEIQAQHILLENEEDVAEVQQKIEDGEDFGELAQEYSTDTGSA
CCCCCHHHHHHHHHHCCCCEEHHHHEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
ENGGDLGYFSAGSMVPEFEEAAFSLEAGEISDPVQSTHGTHIIKVNDVREKEESIGEFED
CCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCHHHHHHHHCHHHH
VKKELEREILLNRVDQTQIQEKINKLIQDAGVQINVEGMEDLFEAEETEENTTEEDAQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12235376