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The map label for this gene is carA [H]

Identifier: 23098534

GI number: 23098534

Start: 1122441

End: 1123529

Strand: Direct

Name: carA [H]

Synonym: OB1079

Alternate gene names: 23098534

Gene position: 1122441-1123529 (Clockwise)

Preceding gene: 23098533

Following gene: 23098535

Centisome position: 30.92

GC content: 36.46

Gene sequence:

>1089_bases
ATGGATAAAGGTTTTTTAGTATTAGAAACCGGAGAAGTATTTGAAGGAATTCTAATTGGAGATAAACAGGCAATCGAAGG
TGAACTTGTTTTTAATACCGGTATGACAGGGTATCAGGAGATGATGACCGACCCTTCTTATAAAGGTCAAATTCTTACAT
TTTGTTATCCGATTATTGGGAATTATGGGATTAATGATATCGATGATGAGAGTAAAGAAATTGCTGTTTCTGCTGTTATT
GCTAGTGATATTTGTGATGAACCTAGTCACTATCAGTTAACAAGGTCATTTTCAAAACAATTAGAGCAAGCTGGTATTCC
AGGTTTGTTTGAAATTGATACACGTCAGCTTGTCATGATTATACGTGAACACGGTTCTTTGCGAGCAAAAATAGTGACCG
ACGAATGGGAAACAAAAAATATAACCTGGCAAACACGTAGCTTATTGTCATTAGTTAATAGTGTGTCTGTTAAACAATCA
GAAACCTATGGTACAGGTGATACTCATATTGCGATTATGGATTACGGATATAAAAAATCAATTCGAAATGCGCTACTTCA
ACAAGGTTGTAAAGTGACGGTTGTTTCTTATCAAACACCACTTCATCACATAAAAGAACTGAACCCTGATGGTATTGTTC
TTAGTAATGGGCCCGGAGATCCAATGGAGTTACAAGCGTATTTTCCTGTTATCAAACAAATAACACAAGACTACCCAACA
CTTGGTATTTGTCTGGGACATCAATTAATTGCATTAGCTTATGGGGCAATGACAAAGAAAATGCATTTTGGTCATCGAGG
CGGTAATCATCCAGTGAAAGATTTATTCACCGGAAAGGTCTGGATAACGTCTCAAAATCACGGATATGTAGTAGAGGAAG
ACACCATTGATTATATGCAGTTTGATGTATTATTTAAGAATGTAAATGATCGTTCTTTAGAAGGTTTGAAACATTGTCAC
TATCCCGTTACTACTGTGCAATTTCATCCGGAGGCACATCCAGGACCAAGTGATACCAATTATATTTTTGAAGATTTCTT
AAATGAAGTGAGAGAAAAGAAAGGAGCATTGACGCATGCCAAAGCGTAA

Upstream 100 bases:

>100_bases
GTAGTACGTATATTACCTCCATTAACGACAACAAAAGAGGAACTACAAATATGTATACAAGCGTTGAAAGAAGTGGCATT
AGAACTGGGGGGAAATACAA

Downstream 100 bases:

>100_bases
GGACATAAACAAGGTACTCGTTATCGGATCGGGTCCAATCATCATCGGGCAGGCGGCAGAGTTCGACTATGCAGGTACAC
AAGCATGTCTTGCTTTAAAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MDKGFLVLETGEVFEGILIGDKQAIEGELVFNTGMTGYQEMMTDPSYKGQILTFCYPIIGNYGINDIDDESKEIAVSAVI
ASDICDEPSHYQLTRSFSKQLEQAGIPGLFEIDTRQLVMIIREHGSLRAKIVTDEWETKNITWQTRSLLSLVNSVSVKQS
ETYGTGDTHIAIMDYGYKKSIRNALLQQGCKVTVVSYQTPLHHIKELNPDGIVLSNGPGDPMELQAYFPVIKQITQDYPT
LGICLGHQLIALAYGAMTKKMHFGHRGGNHPVKDLFTGKVWITSQNHGYVVEEDTIDYMQFDVLFKNVNDRSLEGLKHCH
YPVTTVQFHPEAHPGPSDTNYIFEDFLNEVREKKGALTHAKA

Sequences:

>Translated_362_residues
MDKGFLVLETGEVFEGILIGDKQAIEGELVFNTGMTGYQEMMTDPSYKGQILTFCYPIIGNYGINDIDDESKEIAVSAVI
ASDICDEPSHYQLTRSFSKQLEQAGIPGLFEIDTRQLVMIIREHGSLRAKIVTDEWETKNITWQTRSLLSLVNSVSVKQS
ETYGTGDTHIAIMDYGYKKSIRNALLQQGCKVTVVSYQTPLHHIKELNPDGIVLSNGPGDPMELQAYFPVIKQITQDYPT
LGICLGHQLIALAYGAMTKKMHFGHRGGNHPVKDLFTGKVWITSQNHGYVVEEDTIDYMQFDVLFKNVNDRSLEGLKHCH
YPVTTVQFHPEAHPGPSDTNYIFEDFLNEVREKKGALTHAKA
>Mature_362_residues
MDKGFLVLETGEVFEGILIGDKQAIEGELVFNTGMTGYQEMMTDPSYKGQILTFCYPIIGNYGINDIDDESKEIAVSAVI
ASDICDEPSHYQLTRSFSKQLEQAGIPGLFEIDTRQLVMIIREHGSLRAKIVTDEWETKNITWQTRSLLSLVNSVSVKQS
ETYGTGDTHIAIMDYGYKKSIRNALLQQGCKVTVVSYQTPLHHIKELNPDGIVLSNGPGDPMELQAYFPVIKQITQDYPT
LGICLGHQLIALAYGAMTKKMHFGHRGGNHPVKDLFTGKVWITSQNHGYVVEEDTIDYMQFDVLFKNVNDRSLEGLKHCH
YPVTTVQFHPEAHPGPSDTNYIFEDFLNEVREKKGALTHAKA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=360, Percent_Identity=41.9444444444444, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI169790915, Length=364, Percent_Identity=35.7142857142857, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI21361331, Length=364, Percent_Identity=35.7142857142857, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=37.5661375661376, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789760, Length=138, Percent_Identity=34.0579710144928, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=369, Percent_Identity=41.1924119241192, Blast_Score=267, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=36.6754617414248, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=35.7142857142857, Blast_Score=239, Evalue=6e-64,
Organism=Drosophila melanogaster, GI45555749, Length=375, Percent_Identity=35.2, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=35.2, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40610; Mature: 40610

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKGFLVLETGEVFEGILIGDKQAIEGELVFNTGMTGYQEMMTDPSYKGQILTFCYPIIG
CCCCEEEEECCCHHCEEEECCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCEEEHHHHC
NYGINDIDDESKEIAVSAVIASDICDEPSHYQLTRSFSKQLEQAGIPGLFEIDTRQLVMI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHH
IREHGSLRAKIVTDEWETKNITWQTRSLLSLVNSVSVKQSETYGTGDTHIAIMDYGYKKS
HHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHH
IRNALLQQGCKVTVVSYQTPLHHIKELNPDGIVLSNGPGDPMELQAYFPVIKQITQDYPT
HHHHHHHCCCEEEEEEECCHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCE
LGICLGHQLIALAYGAMTKKMHFGHRGGNHPVKDLFTGKVWITSQNHGYVVEEDTIDYMQ
EHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCEEEECCCHHHHH
FDVLFKNVNDRSLEGLKHCHYPVTTVQFHPEAHPGPSDTNYIFEDFLNEVREKKGALTHA
HHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
KA
CC
>Mature Secondary Structure
MDKGFLVLETGEVFEGILIGDKQAIEGELVFNTGMTGYQEMMTDPSYKGQILTFCYPIIG
CCCCEEEEECCCHHCEEEECCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCEEEHHHHC
NYGINDIDDESKEIAVSAVIASDICDEPSHYQLTRSFSKQLEQAGIPGLFEIDTRQLVMI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHH
IREHGSLRAKIVTDEWETKNITWQTRSLLSLVNSVSVKQSETYGTGDTHIAIMDYGYKKS
HHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHH
IRNALLQQGCKVTVVSYQTPLHHIKELNPDGIVLSNGPGDPMELQAYFPVIKQITQDYPT
HHHHHHHCCCEEEEEEECCHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCE
LGICLGHQLIALAYGAMTKKMHFGHRGGNHPVKDLFTGKVWITSQNHGYVVEEDTIDYMQ
EHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCEEEECCCHHHHH
FDVLFKNVNDRSLEGLKHCHYPVTTVQFHPEAHPGPSDTNYIFEDFLNEVREKKGALTHA
HHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]