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The map label for this gene is thiD [H]

Identifier: 23098495

GI number: 23098495

Start: 1080819

End: 1081649

Strand: Reverse

Name: thiD [H]

Synonym: OB1040

Alternate gene names: 23098495

Gene position: 1081649-1080819 (Counterclockwise)

Preceding gene: 23098496

Following gene: 23098492

Centisome position: 29.79

GC content: 36.7

Gene sequence:

>831_bases
ATGACGATGAAAAAGACATTAACACTCGCTGGATCAGATTCTAGTGGTGGTGCAGGTATAGCAGCTGATTTAAAGACTTT
CCAGGAATTAAATGTATACGGCATGACTGCGTTAACTTCTATCGTTTCCATGAATCCACAAGGATGGAATCATCATGTTA
CACCAATTGATGTGGAAGTAGTAGAAAAGCAACTAGATACCATATTATCAGTAGGCGTAGATGCTATGAAATCCGGTATG
CTTGGATCTGTAGATATTATCAATTTAGGGGCAAGAAAAATTGATGAATTTAATATAAAACAATATGTTTTAGACCCTGT
AATGGTTTGTAAGGGAGACGATGAAGTATTGCAACCAGAAAATACAGATGCGATGCGTGAGGAGTTATTACCACGTTCCC
TAGTTACTACACCAAATCTTTTTGAAGCAGCACAATTAGCTAAAACAAAACCGATTAAAACGTTGGAGCAAATGAAAGAT
GCTGCAATAAAAATCCATGAATTAGGCGCCAAAAATGTTGTAATCAAAGGCGGCAAAGATTTAGAACATGATAAGGCAGT
AGACCTTTTCTATGATGGAACTCGCTTTGAGTTATTGGAAAGTGAAAAAATCGACACTACCTATAACCATGGTGCAGGTT
GTACATTTGCAGCTGCTATTACAGCTAATCTAGCTCATGGTATGGATGTTTTAGAAGCTGTTAAAACTGCTAAATCATTT
GTTACATCTGCAATCGCAAATGGTTGGAAGTTAAATCAATACGTAGGACCAGTTATGCATGGTGCTTATAATCGAATAGA
TAGCACTAAAGTAGAAATGTCTTCTATTTAG

Upstream 100 bases:

>100_bases
GCTATGACTTTACAACTAGTTCATAAAGGATTATTCTTTAACTATCAAATATTTATTGAAGTGCGAATTTAGAGCAGAGC
AACAAAGGAGGAAACAAACG

Downstream 100 bases:

>100_bases
GAACAAAACGTAAGCGAATAATTTAGTACGTCTATTCACGGATGCAAAATTTTCATAGATCGTAAAAAAAGCTTGGACAG
CGTGTCCAAGCTTTTTTAGT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTMKKTLTLAGSDSSGGAGIAADLKTFQELNVYGMTALTSIVSMNPQGWNHHVTPIDVEVVEKQLDTILSVGVDAMKSGM
LGSVDIINLGARKIDEFNIKQYVLDPVMVCKGDDEVLQPENTDAMREELLPRSLVTTPNLFEAAQLAKTKPIKTLEQMKD
AAIKIHELGAKNVVIKGGKDLEHDKAVDLFYDGTRFELLESEKIDTTYNHGAGCTFAAAITANLAHGMDVLEAVKTAKSF
VTSAIANGWKLNQYVGPVMHGAYNRIDSTKVEMSSI

Sequences:

>Translated_276_residues
MTMKKTLTLAGSDSSGGAGIAADLKTFQELNVYGMTALTSIVSMNPQGWNHHVTPIDVEVVEKQLDTILSVGVDAMKSGM
LGSVDIINLGARKIDEFNIKQYVLDPVMVCKGDDEVLQPENTDAMREELLPRSLVTTPNLFEAAQLAKTKPIKTLEQMKD
AAIKIHELGAKNVVIKGGKDLEHDKAVDLFYDGTRFELLESEKIDTTYNHGAGCTFAAAITANLAHGMDVLEAVKTAKSF
VTSAIANGWKLNQYVGPVMHGAYNRIDSTKVEMSSI
>Mature_275_residues
TMKKTLTLAGSDSSGGAGIAADLKTFQELNVYGMTALTSIVSMNPQGWNHHVTPIDVEVVEKQLDTILSVGVDAMKSGML
GSVDIINLGARKIDEFNIKQYVLDPVMVCKGDDEVLQPENTDAMREELLPRSLVTTPNLFEAAQLAKTKPIKTLEQMKDA
AIKIHELGAKNVVIKGGKDLEHDKAVDLFYDGTRFELLESEKIDTTYNHGAGCTFAAAITANLAHGMDVLEAVKTAKSFV
TSAIANGWKLNQYVGPVMHGAYNRIDSTKVEMSSI

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=35.7414448669202, Blast_Score=155, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=29.6703296703297, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=29.5620437956204, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324517, Length=276, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 29917; Mature: 29785

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKKTLTLAGSDSSGGAGIAADLKTFQELNVYGMTALTSIVSMNPQGWNHHVTPIDVEV
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
VEKQLDTILSVGVDAMKSGMLGSVDIINLGARKIDEFNIKQYVLDPVMVCKGDDEVLQPE
HHHHHHHHHHHCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
NTDAMREELLPRSLVTTPNLFEAAQLAKTKPIKTLEQMKDAAIKIHELGAKNVVIKGGKD
CCHHHHHHHCCHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
LEHDKAVDLFYDGTRFELLESEKIDTTYNHGAGCTFAAAITANLAHGMDVLEAVKTAKSF
CCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VTSAIANGWKLNQYVGPVMHGAYNRIDSTKVEMSSI
HHHHHHCCEECHHHHHHHHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
TMKKTLTLAGSDSSGGAGIAADLKTFQELNVYGMTALTSIVSMNPQGWNHHVTPIDVEV
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
VEKQLDTILSVGVDAMKSGMLGSVDIINLGARKIDEFNIKQYVLDPVMVCKGDDEVLQPE
HHHHHHHHHHHCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
NTDAMREELLPRSLVTTPNLFEAAQLAKTKPIKTLEQMKDAAIKIHELGAKNVVIKGGKD
CCHHHHHHHCCHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
LEHDKAVDLFYDGTRFELLESEKIDTTYNHGAGCTFAAAITANLAHGMDVLEAVKTAKSF
CCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VTSAIANGWKLNQYVGPVMHGAYNRIDSTKVEMSSI
HHHHHHCCEECHHHHHHHHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA