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The map label for this gene is mscL

Identifier: 23098476

GI number: 23098476

Start: 1063133

End: 1063525

Strand: Direct

Name: mscL

Synonym: OB1021

Alternate gene names: 23098476

Gene position: 1063133-1063525 (Clockwise)

Preceding gene: 23098475

Following gene: 23098477

Centisome position: 29.28

GC content: 31.81

Gene sequence:

>393_bases
GTGTGGAAAGAGTTTAAAGAATTTGCTTTTAAAGGAAATATAATAGACTTAGCCGTAGCCGTTGTCATCGGTGGTGCATT
TGGAGCCATTGTGACGTCATTTGTGGAAAATATAATTACTCCGTTAATGGGTGTGATAGTTGGAGGAGTGGATTTTACAA
CTTTAAAAGTAACAGTTGGAGAAGCAGAGATATTATATGGTAACTTCATTCAATCATTTGTTGATTTTATTATTATTGCT
TTTTCTATATTTTTAGCTATCAAATTCCTCGTTAAATTCAAACGCCAGAAAGAAGAAGAGGAAGTTGAAGCGGTAGTAGA
AGAATTAAGTAAACAAGAAGAGTTACTTACAGAAATTAGAGATCTACTTAAAGAACAATCAAATAAAAATTAA

Upstream 100 bases:

>100_bases
TTCATATAATTTATATTATATATTTTGGGAGTCTCTTGACGGGATAAAACCTTCCTTTTATTCTGTTAATATACTGAATT
AGATTGAGAGGTGAATGAAG

Downstream 100 bases:

>100_bases
TTTTGAGGTTGTCCTATTAAAAAAATGGGGTAACCTTTTTTATTTAGGTATGCAGAAGGAGATAATTTCCGTCAAGAGAA
ACAGGATAAATAATCTGATA

Product: large conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MWKEFKEFAFKGNIIDLAVAVVIGGAFGAIVTSFVENIITPLMGVIVGGVDFTTLKVTVGEAEILYGNFIQSFVDFIIIA
FSIFLAIKFLVKFKRQKEEEEVEAVVEELSKQEELLTEIRDLLKEQSNKN

Sequences:

>Translated_130_residues
MWKEFKEFAFKGNIIDLAVAVVIGGAFGAIVTSFVENIITPLMGVIVGGVDFTTLKVTVGEAEILYGNFIQSFVDFIIIA
FSIFLAIKFLVKFKRQKEEEEVEAVVEELSKQEELLTEIRDLLKEQSNKN
>Mature_130_residues
MWKEFKEFAFKGNIIDLAVAVVIGGAFGAIVTSFVENIITPLMGVIVGGVDFTTLKVTVGEAEILYGNFIQSFVDFIIIA
FSIFLAIKFLVKFKRQKEEEEVEAVVEELSKQEELLTEIRDLLKEQSNKN

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=135, Percent_Identity=51.1111111111111, Blast_Score=136, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_OCEIH (Q8CUT6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691942.1
- ProteinModelPortal:   Q8CUT6
- SMR:   Q8CUT6
- GeneID:   1016880
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1021
- NMPDR:   fig|221109.1.peg.1023
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- BioCyc:   OIHE221109:OB1021-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14596; Mature: 14596

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc5b7234)-; HASH(0xde60598)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKEFKEFAFKGNIIDLAVAVVIGGAFGAIVTSFVENIITPLMGVIVGGVDFTTLKVTVG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEC
EAEILYGNFIQSFVDFIIIAFSIFLAIKFLVKFKRQKEEEEVEAVVEELSKQEELLTEIR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLKEQSNKN
HHHHHHCCCH
>Mature Secondary Structure
MWKEFKEFAFKGNIIDLAVAVVIGGAFGAIVTSFVENIITPLMGVIVGGVDFTTLKVTVG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEC
EAEILYGNFIQSFVDFIIIAFSIFLAIKFLVKFKRQKEEEEVEAVVEELSKQEELLTEIR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLKEQSNKN
HHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12235376