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The map label for this gene is opuAB [H]

Identifier: 23098455

GI number: 23098455

Start: 1044022

End: 1044852

Strand: Direct

Name: opuAB [H]

Synonym: OB1000

Alternate gene names: 23098455

Gene position: 1044022-1044852 (Clockwise)

Preceding gene: 23098454

Following gene: 23098456

Centisome position: 28.76

GC content: 36.82

Gene sequence:

>831_bases
ATGGATTATTTTTATTTACCACTAGAACAGTGGATGAATGAATTCGTTAATGGATGGTTTCTACCAACGTTTAGTGGTAT
ATTCAACGTCATTAGTGAAGTTTTTAACATATTTATCGGTGGAGTTACAGATTTATTATTATTGATCCCAGCAGAACTAT
TTACTATTATTCTAGCATTAATAGCTTGGAAGGTATCTGGCATTGGTTTAGCTGTTTTTACTATAATAGGATTTTTATTT
ATTGGTTCTGTGGATATGTGGGAAGGAGCTATGCAGACACTTGCAATTATTATTGTAGCAACCCTTGTCTCTCTCGTTAT
AGGTATACCTGTGGGAATATTAAGCGCCATGAATGATGGAGTACAACGAATCGTGCGACCAATCCTAGATTTCATGCAAA
CTTTACCGAGTTTTGTGTACCTAATTCCTGCAGTCTTATTATTTGGTCTAGGCGGTGTCCCAGCAGTGATGGCTACATTT
ATATTTGCTGCTCCTCCAGCTGTTCGCATGACTAATTTAGGTATTCGAAGTGTACCAAAAGAGATTGTTGAAGCTTCTCG
AGCATTTGGTTCTACAAATAAGCAACTATTATATAAAGTTCAAATTCCATTAGCGATACCAAGTATCATGGCAGGAGTAA
ATCAAACAATTATGCTTTCCCTGTCAATGGCTGTTGTGGCTTCTATGATCGGAGCACCAGGATTAGGTGCATCTGTATTA
ACAGCTATTTCTCAAGTGAATATTGGATTAGGTTTGGTAGCTGGTTTGGGAATTGTTGTCTTAGCAATTGTTCTTGATCG
GATTACACAAGGAATTGGTAAAGGAAAATAA

Upstream 100 bases:

>100_bases
TCGCCGTTGTTGAAGAAGACAAGTTAGTTGGGATTGTATCACGAGTATCCATTCTATCAGGTCTAGTTCTAGGAAAGGAA
AACGCGGAGGTGAATGAATA

Downstream 100 bases:

>100_bases
TCGATGAAAGGATTGTTTAACATAATGAAGAAGTTTACTATTGTTTTATCACTGTTTACATTACTATTTTTAGCAGCCTG
TGGATCTAGTAATGAAGAAT

Product: glycine betaine ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDYFYLPLEQWMNEFVNGWFLPTFSGIFNVISEVFNIFIGGVTDLLLLIPAELFTIILALIAWKVSGIGLAVFTIIGFLF
IGSVDMWEGAMQTLAIIIVATLVSLVIGIPVGILSAMNDGVQRIVRPILDFMQTLPSFVYLIPAVLLFGLGGVPAVMATF
IFAAPPAVRMTNLGIRSVPKEIVEASRAFGSTNKQLLYKVQIPLAIPSIMAGVNQTIMLSLSMAVVASMIGAPGLGASVL
TAISQVNIGLGLVAGLGIVVLAIVLDRITQGIGKGK

Sequences:

>Translated_276_residues
MDYFYLPLEQWMNEFVNGWFLPTFSGIFNVISEVFNIFIGGVTDLLLLIPAELFTIILALIAWKVSGIGLAVFTIIGFLF
IGSVDMWEGAMQTLAIIIVATLVSLVIGIPVGILSAMNDGVQRIVRPILDFMQTLPSFVYLIPAVLLFGLGGVPAVMATF
IFAAPPAVRMTNLGIRSVPKEIVEASRAFGSTNKQLLYKVQIPLAIPSIMAGVNQTIMLSLSMAVVASMIGAPGLGASVL
TAISQVNIGLGLVAGLGIVVLAIVLDRITQGIGKGK
>Mature_276_residues
MDYFYLPLEQWMNEFVNGWFLPTFSGIFNVISEVFNIFIGGVTDLLLLIPAELFTIILALIAWKVSGIGLAVFTIIGFLF
IGSVDMWEGAMQTLAIIIVATLVSLVIGIPVGILSAMNDGVQRIVRPILDFMQTLPSFVYLIPAVLLFGLGGVPAVMATF
IFAAPPAVRMTNLGIRSVPKEIVEASRAFGSTNKQLLYKVQIPLAIPSIMAGVNQTIMLSLSMAVVASMIGAPGLGASVL
TAISQVNIGLGLVAGLGIVVLAIVLDRITQGIGKGK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=277, Percent_Identity=48.014440433213, Blast_Score=250, Evalue=7e-68,
Organism=Escherichia coli, GI1788451, Length=199, Percent_Identity=30.6532663316583, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788449, Length=174, Percent_Identity=28.1609195402299, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786564, Length=161, Percent_Identity=27.9503105590062, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29310; Mature: 29310

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFYLPLEQWMNEFVNGWFLPTFSGIFNVISEVFNIFIGGVTDLLLLIPAELFTIILAL
CCEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAWKVSGIGLAVFTIIGFLFIGSVDMWEGAMQTLAIIIVATLVSLVIGIPVGILSAMNDG
HHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQRIVRPILDFMQTLPSFVYLIPAVLLFGLGGVPAVMATFIFAAPPAVRMTNLGIRSVPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHH
EIVEASRAFGSTNKQLLYKVQIPLAIPSIMAGVNQTIMLSLSMAVVASMIGAPGLGASVL
HHHHHHHHHCCCCHHEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
TAISQVNIGLGLVAGLGIVVLAIVLDRITQGIGKGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDYFYLPLEQWMNEFVNGWFLPTFSGIFNVISEVFNIFIGGVTDLLLLIPAELFTIILAL
CCEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAWKVSGIGLAVFTIIGFLFIGSVDMWEGAMQTLAIIIVATLVSLVIGIPVGILSAMNDG
HHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQRIVRPILDFMQTLPSFVYLIPAVLLFGLGGVPAVMATFIFAAPPAVRMTNLGIRSVPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHH
EIVEASRAFGSTNKQLLYKVQIPLAIPSIMAGVNQTIMLSLSMAVVASMIGAPGLGASVL
HHHHHHHHHCCCCHHEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
TAISQVNIGLGLVAGLGIVVLAIVLDRITQGIGKGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]