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The map label for this gene is speE

Identifier: 23098398

GI number: 23098398

Start: 988742

End: 990280

Strand: Direct

Name: speE

Synonym: OB0943

Alternate gene names: 23098398

Gene position: 988742-990280 (Clockwise)

Preceding gene: 23098397

Following gene: 23098399

Centisome position: 27.23

GC content: 36.91

Gene sequence:

>1539_bases
ATGTTGAATGATAAAGCAATTAGACAAAGTAAAATTATTTATTGGTCCTCGGGAATTGTGTCCATTTGTGGTATTATTTT
CGAGGTTTTATTTGGTGCGTTAGGTTCCTACATATTAGGTGATGGTGTTAAACAATATACACTTACGATCTCCTTATTCT
TAACAGGTATGGGAATAGGAGCTAGCTTAAGTGAGAAGTTTATGCGGAATCTGATCATTAAGTTTGTTTGGATTGAATTT
TGTGTAGCATTAATTGGTGGTTTTTCTAGTTTTATTATGTTTGGTATAACCGCTTTTGCACCAGCTGGAACGGATGCGTT
TTACTTATATTCTATTACGCTAATCATTGGTGCCTTAACAGGAGTTGAGCTACCAATATTAATTCGTAAAGCAAATGAAA
TCGGTGTGACGTTAAATAAAAGTACCGCTCGTGTGTTATTTTCTGATTATGCAGGGGGACTAATCGGCGGGGTATTATTT
GTCTTCTTATTCCGACCTTATTTTGGTATGGTAAAAACAGCATTTTTAGTTGGATTAATTAATTTAACAGTTGCATTAAT
TGTCTTATGGTTATTTAGAAAAGAGATTCGGCATTTTATCGTACATGCTGTCATCGGGGGAGTCATTGGTGTGTTACTAA
TAGCCGGATTGTTTTTCGGTGAAGAAATGGCGTTTAATTTTGAACAAAAATTGTATCAAGATCCAATAATTCATATGGAA
GAGAGTAGCTATCAAAAAATCACAGTTACACAAGATGAGAAAGATATTCGACTTTATTTGGATGGATCTTTGCAATTTAG
CTCGGTCGATGAGCATCGCTATCATGAAGTGCTCGTCCATCCGGCCATGGCAAATGTAGAGACTCCAGAGAATGTCCTTA
TCCTTGGTGGCGGCGATGGAATTGCAGCTAAGGAAGTTTTAAAGTATCAAGATGTGAAACAGGTTACGCTCGTTGACTTA
GATCCAGCAGTTGTGGAGTTGGCAAATGAGAATCGGCATTTGCTAGAGATTAATGAAGGGGCCTTAATGGATGAAAAAGT
AGAAGTGAAAAACATGGACGCCTTTCAATTTTTAGAAGATACATCGGAATGGTATGATGTTATTCTTGTAGATTTGCCTG
ACCCAAACAATGAAAGTCTAAATAAGTTGTACACGAAGGAATTTTATTCCTTGGTACGCAATCACTTAAAACCTGAGGGG
ACACTCATGGTTCAAGCAACAAGTCCAGTTTTCGCCAGAGAGGTATATTGGACGATTTCAGAGACGATTAGTTCAACGAA
TCTGAATACGGAGAATTTACATGTAGATGTACCGAGTTTTGGTAATTGGGGATTTGTTATGGCAAGTCGTGAGGAGATTG
ATTTAGATATAATGGAAATACCGGTTTCTACTAGATTTTTAACAGATGATATGATGCCAGCACTAACTGCATTTGGCAAG
GACGAAGATCAACAGATACCGAACTTTGAGTTAAAAGCAAATACGCTTATTGACCCTCACTTGATTCAAATTTATGAAAA
AGCATGGGAGAATTATTAA

Upstream 100 bases:

>100_bases
ATTGGCGCATCGATTACGTAAAAATATGAGAGTTTACAGCCAGGTATTTGCTTGGCTGTTTATCGTTTTATGGCTTTTTT
CTTTATAGTGGAGTGGATGA

Downstream 100 bases:

>100_bases
CTTTGGACAAGGAATAAGTGGATATATTAGCCTTAAAAGCGATACCTATTGCAGCGTATCGCTTTGTTTGTATATCGTAG
GAAATAGACTTACCTTTTAC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLNDKAIRQSKIIYWSSGIVSICGIIFEVLFGALGSYILGDGVKQYTLTISLFLTGMGIGASLSEKFMRNLIIKFVWIEF
CVALIGGFSSFIMFGITAFAPAGTDAFYLYSITLIIGALTGVELPILIRKANEIGVTLNKSTARVLFSDYAGGLIGGVLF
VFLFRPYFGMVKTAFLVGLINLTVALIVLWLFRKEIRHFIVHAVIGGVIGVLLIAGLFFGEEMAFNFEQKLYQDPIIHME
ESSYQKITVTQDEKDIRLYLDGSLQFSSVDEHRYHEVLVHPAMANVETPENVLILGGGDGIAAKEVLKYQDVKQVTLVDL
DPAVVELANENRHLLEINEGALMDEKVEVKNMDAFQFLEDTSEWYDVILVDLPDPNNESLNKLYTKEFYSLVRNHLKPEG
TLMVQATSPVFAREVYWTISETISSTNLNTENLHVDVPSFGNWGFVMASREEIDLDIMEIPVSTRFLTDDMMPALTAFGK
DEDQQIPNFELKANTLIDPHLIQIYEKAWENY

Sequences:

>Translated_512_residues
MLNDKAIRQSKIIYWSSGIVSICGIIFEVLFGALGSYILGDGVKQYTLTISLFLTGMGIGASLSEKFMRNLIIKFVWIEF
CVALIGGFSSFIMFGITAFAPAGTDAFYLYSITLIIGALTGVELPILIRKANEIGVTLNKSTARVLFSDYAGGLIGGVLF
VFLFRPYFGMVKTAFLVGLINLTVALIVLWLFRKEIRHFIVHAVIGGVIGVLLIAGLFFGEEMAFNFEQKLYQDPIIHME
ESSYQKITVTQDEKDIRLYLDGSLQFSSVDEHRYHEVLVHPAMANVETPENVLILGGGDGIAAKEVLKYQDVKQVTLVDL
DPAVVELANENRHLLEINEGALMDEKVEVKNMDAFQFLEDTSEWYDVILVDLPDPNNESLNKLYTKEFYSLVRNHLKPEG
TLMVQATSPVFAREVYWTISETISSTNLNTENLHVDVPSFGNWGFVMASREEIDLDIMEIPVSTRFLTDDMMPALTAFGK
DEDQQIPNFELKANTLIDPHLIQIYEKAWENY
>Mature_512_residues
MLNDKAIRQSKIIYWSSGIVSICGIIFEVLFGALGSYILGDGVKQYTLTISLFLTGMGIGASLSEKFMRNLIIKFVWIEF
CVALIGGFSSFIMFGITAFAPAGTDAFYLYSITLIIGALTGVELPILIRKANEIGVTLNKSTARVLFSDYAGGLIGGVLF
VFLFRPYFGMVKTAFLVGLINLTVALIVLWLFRKEIRHFIVHAVIGGVIGVLLIAGLFFGEEMAFNFEQKLYQDPIIHME
ESSYQKITVTQDEKDIRLYLDGSLQFSSVDEHRYHEVLVHPAMANVETPENVLILGGGDGIAAKEVLKYQDVKQVTLVDL
DPAVVELANENRHLLEINEGALMDEKVEVKNMDAFQFLEDTSEWYDVILVDLPDPNNESLNKLYTKEFYSLVRNHLKPEG
TLMVQATSPVFAREVYWTISETISSTNLNTENLHVDVPSFGNWGFVMASREEIDLDIMEIPVSTRFLTDDMMPALTAFGK
DEDQQIPNFELKANTLIDPHLIQIYEKAWENY

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=246, Percent_Identity=25.609756097561, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786312, Length=186, Percent_Identity=29.5698924731183, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI212645616, Length=229, Percent_Identity=27.9475982532751, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25154106, Length=181, Percent_Identity=32.5966850828729, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323175, Length=242, Percent_Identity=29.7520661157025, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325326, Length=239, Percent_Identity=29.2887029288703, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24645443, Length=247, Percent_Identity=29.9595141700405, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355941, Length=171, Percent_Identity=30.9941520467836, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_OCEIH (Q8CV14)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691864.1
- ProteinModelPortal:   Q8CV14
- SMR:   Q8CV14
- GeneID:   1016686
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0943
- NMPDR:   fig|221109.1.peg.945
- HOGENOM:   HBG564037
- OMA:   YAKATRF
- ProtClustDB:   PRK03612
- BioCyc:   OIHE221109:OB0943-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 57494; Mature: 57494

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 245-245 BINDING 299-299 BINDING 319-319 BINDING 371-371 BINDING 374-374

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDKAIRQSKIIYWSSGIVSICGIIFEVLFGALGSYILGDGVKQYTLTISLFLTGMGIG
CCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCC
ASLSEKFMRNLIIKFVWIEFCVALIGGFSSFIMFGITAFAPAGTDAFYLYSITLIIGALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GVELPILIRKANEIGVTLNKSTARVLFSDYAGGLIGGVLFVFLFRPYFGMVKTAFLVGLI
CCCEEEEEEECCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLTVALIVLWLFRKEIRHFIVHAVIGGVIGVLLIAGLFFGEEMAFNFEQKLYQDPIIHME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHCCCCEEEC
ESSYQKITVTQDEKDIRLYLDGSLQFSSVDEHRYHEVLVHPAMANVETPENVLILGGGDG
CCCCEEEEEEECCCCEEEEEECCEEECCCCHHHHHHHEEECHHCCCCCCCCEEEEECCCC
IAAKEVLKYQDVKQVTLVDLDPAVVELANENRHLLEINEGALMDEKVEVKNMDAFQFLED
HHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCHHCCCHHHHHHHC
TSEWYDVILVDLPDPNNESLNKLYTKEFYSLVRNHLKPEGTLMVQATSPVFAREVYWTIS
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
ETISSTNLNTENLHVDVPSFGNWGFVMASREEIDLDIMEIPVSTRFLTDDMMPALTAFGK
HHHHHCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
DEDQQIPNFELKANTLIDPHLIQIYEKAWENY
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNDKAIRQSKIIYWSSGIVSICGIIFEVLFGALGSYILGDGVKQYTLTISLFLTGMGIG
CCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCC
ASLSEKFMRNLIIKFVWIEFCVALIGGFSSFIMFGITAFAPAGTDAFYLYSITLIIGALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GVELPILIRKANEIGVTLNKSTARVLFSDYAGGLIGGVLFVFLFRPYFGMVKTAFLVGLI
CCCEEEEEEECCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLTVALIVLWLFRKEIRHFIVHAVIGGVIGVLLIAGLFFGEEMAFNFEQKLYQDPIIHME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHCCCCEEEC
ESSYQKITVTQDEKDIRLYLDGSLQFSSVDEHRYHEVLVHPAMANVETPENVLILGGGDG
CCCCEEEEEEECCCCEEEEEECCEEECCCCHHHHHHHEEECHHCCCCCCCCEEEEECCCC
IAAKEVLKYQDVKQVTLVDLDPAVVELANENRHLLEINEGALMDEKVEVKNMDAFQFLED
HHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCHHCCCHHHHHHHC
TSEWYDVILVDLPDPNNESLNKLYTKEFYSLVRNHLKPEGTLMVQATSPVFAREVYWTIS
CCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
ETISSTNLNTENLHVDVPSFGNWGFVMASREEIDLDIMEIPVSTRFLTDDMMPALTAFGK
HHHHHCCCCCCEEEEECCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
DEDQQIPNFELKANTLIDPHLIQIYEKAWENY
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376