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The map label for this gene is ycgS [H]

Identifier: 23098380

GI number: 23098380

Start: 977573

End: 978427

Strand: Direct

Name: ycgS [H]

Synonym: OB0925

Alternate gene names: 23098380

Gene position: 977573-978427 (Clockwise)

Preceding gene: 23098379

Following gene: 23098381

Centisome position: 26.93

GC content: 36.61

Gene sequence:

>855_bases
ATGGAGAGAATCATTGGTACATATGACGTAGATAATCAAACTATCGAATATTCAATCATAGGAAAAGAAGGTGTACCAGT
TCTAGTCATGCATGGTGGTCATTCCAGTTGTTATGAGAAATTTGGGTATGGTTCACTCGTTGAGAACGGATTTAAAATTA
TTACACCGTCAAGGGCGGGGTATGGGAAAACTTCAAAGGAAATTGGAGAAAGTTTATCTAAGGCTTGCGATTACTATTTA
GGTCTTTTGAATCATTTGGGCGTTGAAAAAATACATCTTATTGCTGTTTCAGCTGGTGGACCAAGTGGTCTTTATTTTGC
TTCTCATTACCCAGAAAGAGTCAAAACATTAACGTTACAAAGTGCTGTTACAAAAGAGTGGCACACACCTAAAGACAAAA
TATATAAAATAGCACAGATTCTGTTTCGTCCTTCCATGGAAAGAATGACTTGGAAACTCACTTCAAGCATGAGCAATCGT
TTCCCTAATTTTATGTTTAAACAAATGACACCTTCGTTTAGTAAGCTTCCGTATAAAAAAATAAAAGAAAAGATGGCAGA
CGAAGATGTTGATGAAATCAGACGTATGAACAATCGCCAACAATCAGGGTACGGCTTTTTAATTGATTTATCGCAAACAA
AGGAGATCACTATAAAGGATTTGAAAGCTATATCTTGCCCCACTCTCATCCAACACAGCAAACATGATGGGTCAGTTCCA
TTAGAACATGCTTATTATGCACATCAGCAAATTCCAGATTCGAGATTATGCTTACTTGATACATGGGGGCATCTTATATG
GCTTGGTCAAGGGTCAAAAGAGGTAAATAAAAAACTTATAGAATTCCTGTTATAG

Upstream 100 bases:

>100_bases
AAGGTGCTTATCAGTAAAAAAGACTTGCACCTAATCTTTGAGAAAAGGGCCATTTAATGAAATAACAATTGCTACTAAGA
ACTGATTTAGGGGTGGGATA

Downstream 100 bases:

>100_bases
TATTCAAAACCAATCAAAACCAATCAAAACGACTTAACAGGTTCTTCTGCAATCGGGCGCCATTCCAGAATAAGGCTTGG
CGCTCGTTCCATACATAAGG

Product: aromatic hydrocarbon catabolism protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MERIIGTYDVDNQTIEYSIIGKEGVPVLVMHGGHSSCYEKFGYGSLVENGFKIITPSRAGYGKTSKEIGESLSKACDYYL
GLLNHLGVEKIHLIAVSAGGPSGLYFASHYPERVKTLTLQSAVTKEWHTPKDKIYKIAQILFRPSMERMTWKLTSSMSNR
FPNFMFKQMTPSFSKLPYKKIKEKMADEDVDEIRRMNNRQQSGYGFLIDLSQTKEITIKDLKAISCPTLIQHSKHDGSVP
LEHAYYAHQQIPDSRLCLLDTWGHLIWLGQGSKEVNKKLIEFLL

Sequences:

>Translated_284_residues
MERIIGTYDVDNQTIEYSIIGKEGVPVLVMHGGHSSCYEKFGYGSLVENGFKIITPSRAGYGKTSKEIGESLSKACDYYL
GLLNHLGVEKIHLIAVSAGGPSGLYFASHYPERVKTLTLQSAVTKEWHTPKDKIYKIAQILFRPSMERMTWKLTSSMSNR
FPNFMFKQMTPSFSKLPYKKIKEKMADEDVDEIRRMNNRQQSGYGFLIDLSQTKEITIKDLKAISCPTLIQHSKHDGSVP
LEHAYYAHQQIPDSRLCLLDTWGHLIWLGQGSKEVNKKLIEFLL
>Mature_284_residues
MERIIGTYDVDNQTIEYSIIGKEGVPVLVMHGGHSSCYEKFGYGSLVENGFKIITPSRAGYGKTSKEIGESLSKACDYYL
GLLNHLGVEKIHLIAVSAGGPSGLYFASHYPERVKTLTLQSAVTKEWHTPKDKIYKIAQILFRPSMERMTWKLTSSMSNR
FPNFMFKQMTPSFSKLPYKKIKEKMADEDVDEIRRMNNRQQSGYGFLIDLSQTKEITIKDLKAISCPTLIQHSKHDGSVP
LEHAYYAHQQIPDSRLCLLDTWGHLIWLGQGSKEVNKKLIEFLL

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 32287; Mature: 32287

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIIGTYDVDNQTIEYSIIGKEGVPVLVMHGGHSSCYEKFGYGSLVENGFKIITPSRAG
CCCEEEEEECCCCEEEEEEECCCCCEEEEEECCCHHHHHHCCCHHHHHCCEEEECCCCCC
YGKTSKEIGESLSKACDYYLGLLNHLGVEKIHLIAVSAGGPSGLYFASHYPERVKTLTLQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHCCHHHHHHHHHH
SAVTKEWHTPKDKIYKIAQILFRPSMERMTWKLTSSMSNRFPNFMFKQMTPSFSKLPYKK
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHCCHHH
IKEKMADEDVDEIRRMNNRQQSGYGFLIDLSQTKEITIKDLKAISCPTLIQHSKHDGSVP
HHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCEEHHHHHHHCCCHHHHCCCCCCCCC
LEHAYYAHQQIPDSRLCLLDTWGHLIWLGQGSKEVNKKLIEFLL
HHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHC
>Mature Secondary Structure
MERIIGTYDVDNQTIEYSIIGKEGVPVLVMHGGHSSCYEKFGYGSLVENGFKIITPSRAG
CCCEEEEEECCCCEEEEEEECCCCCEEEEEECCCHHHHHHCCCHHHHHCCEEEECCCCCC
YGKTSKEIGESLSKACDYYLGLLNHLGVEKIHLIAVSAGGPSGLYFASHYPERVKTLTLQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHCCHHHHHHHHHH
SAVTKEWHTPKDKIYKIAQILFRPSMERMTWKLTSSMSNRFPNFMFKQMTPSFSKLPYKK
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHCCHHH
IKEKMADEDVDEIRRMNNRQQSGYGFLIDLSQTKEITIKDLKAISCPTLIQHSKHDGSVP
HHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCCEEHHHHHHHCCCHHHHCCCCCCCCC
LEHAYYAHQQIPDSRLCLLDTWGHLIWLGQGSKEVNKKLIEFLL
HHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]