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The map label for this gene is nemA [H]

Identifier: 23098371

GI number: 23098371

Start: 969149

End: 969547

Strand: Direct

Name: nemA [H]

Synonym: OB0916

Alternate gene names: 23098371

Gene position: 969149-969547 (Clockwise)

Preceding gene: 23098370

Following gene: 23098372

Centisome position: 26.69

GC content: 34.59

Gene sequence:

>399_bases
TTGTCCCTATATAACATTGGAATCAAACCATTCAATATAGGAAAAATTACTTTAAAAAACCGAGTAGGCGTAGCACCAAT
GACACGTATTAGTGCTACCGAAGAAGGTTTTGTAACAAATAAAATGGTTAATTATTATGGATCTTTTGCTGAAGGTGGAT
TTGTACTTATTATTACTGAAGATACTTATATTAATGATAAGTATAGTCAAACGTACTATGATCAACCGGGTATTGCTTTT
GATGAGCAAGCACATGAGTGGAAAAAAGTTGTTGATGCAGTTCATTCAGCAGGATCAAAAATCTTTATGCAGTTACAACA
TACTGGTCCACTATCTCAGGGAAATCGTTTCGCTAATGAAACAGTTGCTCCTTCTTCTATTCAACCAAACCAACAGTAG

Upstream 100 bases:

>100_bases
GGGCGCAATAATGGAACAAAGGTATGCTGGAATTGGGCGCATTTCCGAAGTAACTCCACGGCCTTATTCTCCTAAAACGC
ATTGGTAAGATTAGGCTTTT

Downstream 100 bases:

>100_bases
GGTTGATCGTAATTCATTAACATTAAGAAGATTGAAACTATAGATTAGTTTTATACGTAATTTTTAACAATACCACATTA
ATAAGAAAGGAGCATTCTAT

Product: enoyl reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSLYNIGIKPFNIGKITLKNRVGVAPMTRISATEEGFVTNKMVNYYGSFAEGGFVLIITEDTYINDKYSQTYYDQPGIAF
DEQAHEWKKVVDAVHSAGSKIFMQLQHTGPLSQGNRFANETVAPSSIQPNQQ

Sequences:

>Translated_132_residues
MSLYNIGIKPFNIGKITLKNRVGVAPMTRISATEEGFVTNKMVNYYGSFAEGGFVLIITEDTYINDKYSQTYYDQPGIAF
DEQAHEWKKVVDAVHSAGSKIFMQLQHTGPLSQGNRFANETVAPSSIQPNQQ
>Mature_131_residues
SLYNIGIKPFNIGKITLKNRVGVAPMTRISATEEGFVTNKMVNYYGSFAEGGFVLIITEDTYINDKYSQTYYDQPGIAFD
EQAHEWKKVVDAVHSAGSKIFMQLQHTGPLSQGNRFANETVAPSSIQPNQQ

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=121, Percent_Identity=37.1900826446281, Blast_Score=71, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=103, Percent_Identity=37.8640776699029, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 14713; Mature: 14582

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYNIGIKPFNIGKITLKNRVGVAPMTRISATEEGFVTNKMVNYYGSFAEGGFVLIITE
CCEECCCCCEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEC
DTYINDKYSQTYYDQPGIAFDEQAHEWKKVVDAVHSAGSKIFMQLQHTGPLSQGNRFANE
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
TVAPSSIQPNQQ
CCCCCCCCCCCC
>Mature Secondary Structure 
SLYNIGIKPFNIGKITLKNRVGVAPMTRISATEEGFVTNKMVNYYGSFAEGGFVLIITE
CEECCCCCEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEC
DTYINDKYSQTYYDQPGIAFDEQAHEWKKVVDAVHSAGSKIFMQLQHTGPLSQGNRFANE
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
TVAPSSIQPNQQ
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]