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The map label for this gene is fosB [H]

Identifier: 23098280

GI number: 23098280

Start: 879064

End: 879459

Strand: Direct

Name: fosB [H]

Synonym: OB0825

Alternate gene names: 23098280

Gene position: 879064-879459 (Clockwise)

Preceding gene: 23098279

Following gene: 23098281

Centisome position: 24.21

GC content: 32.83

Gene sequence:

>396_bases
TTGCTGTTTTCTGTATCCAATTTGGAACAATCAATTGACTTTTACCAACAAGTTTTTGATGCAAAGCTATTAGTTAAAGG
TAGGTCAACAGCATATTTTGATTTGAATGGTATATGGTTAGCGTTAAATGAAGAAAAACATATTCCGCGAAATGAAATTA
ATGAATCTTATACCCATACGGCATTCTCCATTGATGAATCAGAGCTTGAAAGTGCTATTCAACATCTAAAAGCGTTAAAT
GTAAACATTCTAGAGGGACGAGAACGAGCTGAACAAGATAAACAATCGATTTATTTTACAGATCCAGATGGGCATAAATT
TGAATTCCATACGGGAACATTGCAGGAAAGGTTAAAATATTATCGGGATCAAAAAAAGCATATGACTTTTTATTAG

Upstream 100 bases:

>100_bases
CATTTCAAGTCTATGGATATAAGCCTAAACCAATATCAGATGTGGTTTGGATAAAAAATGAAGGAGGTCTTTCGTAATTA
ATATTAAAGGGCTGAATCAT

Downstream 100 bases:

>100_bases
ATGTAAAAATGGCAGAGGAATATCTTTCTCCTGCCATTTTTCAATATTGTAGTTCTCTTTTTTGAAAGTAATCAATGATT
AAGCTAAAAGTTAAAGATAA

Product: fosfomycin resistance protein FosB

Products: HX; R-S-glutathione

Alternate protein names: Fosfomycin resistance protein [H]

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MLFSVSNLEQSIDFYQQVFDAKLLVKGRSTAYFDLNGIWLALNEEKHIPRNEINESYTHTAFSIDESELESAIQHLKALN
VNILEGRERAEQDKQSIYFTDPDGHKFEFHTGTLQERLKYYRDQKKHMTFY

Sequences:

>Translated_131_residues
MLFSVSNLEQSIDFYQQVFDAKLLVKGRSTAYFDLNGIWLALNEEKHIPRNEINESYTHTAFSIDESELESAIQHLKALN
VNILEGRERAEQDKQSIYFTDPDGHKFEFHTGTLQERLKYYRDQKKHMTFY
>Mature_131_residues
MLFSVSNLEQSIDFYQQVFDAKLLVKGRSTAYFDLNGIWLALNEEKHIPRNEINESYTHTAFSIDESELESAIQHLKALN
VNILEGRERAEQDKQSIYFTDPDGHKFEFHTGTLQERLKYYRDQKKHMTFY

Specific function: Metallothiol transferase which confers resistance to fosfomycin by catalyzing the addition of a thiol cofactor to fosfomycin. L-cysteine is probably the physiological thiol donor [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family. FosB subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR022858 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 2.5.1.18

Molecular weight: Translated: 15511; Mature: 15511

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSVSNLEQSIDFYQQVFDAKLLVKGRSTAYFDLNGIWLALNEEKHIPRNEINESYTHT
CCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCEEEEECCCCCCCHHHCCCCCCCE
AFSIDESELESAIQHLKALNVNILEGRERAEQDKQSIYFTDPDGHKFEFHTGTLQERLKY
EEECCHHHHHHHHHHHHHCCCEEECCHHHHHHCCCEEEEECCCCCEEEEECCHHHHHHHH
YRDQKKHMTFY
HHHHHHCCCCC
>Mature Secondary Structure
MLFSVSNLEQSIDFYQQVFDAKLLVKGRSTAYFDLNGIWLALNEEKHIPRNEINESYTHT
CCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCEEEEECCCCCCCHHHCCCCCCCE
AFSIDESELESAIQHLKALNVNILEGRERAEQDKQSIYFTDPDGHKFEFHTGTLQERLKY
EEECCHHHHHHHHHHHHHCCCEEECCHHHHHHCCCEEEEECCCCCEEEEECCHHHHHHHH
YRDQKKHMTFY
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]