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The map label for this gene is braE [H]

Identifier: 23098139

GI number: 23098139

Start: 719968

End: 720954

Strand: Direct

Name: braE [H]

Synonym: OB0684

Alternate gene names: 23098139

Gene position: 719968-720954 (Clockwise)

Preceding gene: 23098138

Following gene: 23098140

Centisome position: 19.83

GC content: 34.14

Gene sequence:

>987_bases
ATGATTAAAAATAATACACAAAAATATATCGTTATTGGATTACTCATGGTTGCTATTCTCTTCCCTCTATTCACCCAAAA
TAATTACTATATTCATGTCATGACGCTATCGTTTATATGGATGATCGCCGTATATGGATTAAATATACTGGCGGGATATA
CCGGTTATTTATCATTGGCTCATGCTGGATTCTTTGCAATTGGAGCATATGCATTAGGCTTACTAACCGTAAAGGCAGAA
GTGGGTTTTTGGCTAGCTTTAATTTTAGCACCAATTATAACAACAATCATTGGATTTGGAATTGGTTTAATTGCATTGCG
CACCAAGGAACATTTTTTTGCTATATACACACTTTGTGTAGGGTACGTCATCTATTTATTAATTGATAAGTGGGATAGTT
TAACAGAAGGAGTTCGCGGACTAATGGGCATACCGTTACCATCTGATATTGGTCCAATCTCTTTTGAGAGTAATATCGCT
CAATACTATTTCATTTTAATCGTATTGCTTTTCTCTATTTTCGTGATTTATAGAATTGTAAATTCCATAGTCGGAAAAAA
GCTAATCGCAATTCGTAATAGTGAACAACTCGCTTTATCATTAGGTATTTCAACGAAAAAAAATAAACTACTTGCTTTTG
TATTATCTACGTTTTTTGCAGGATTAGCGGGTGCGTTATATGCAAGTTTTATACGGTTCCTAGGTCCAGATATCGGTTCT
ACAAATATAACATTTGATCTACTCATGTATTTAATTGTCGGAGGTATTGGTACATTATCTGGTCCGATTATAGGTACTCT
ATTAGTCGTATGGCTTTCTCAAAATCTACAATTCTTACAAGATTATCGAATGCTAATATTTGGTCCATTATTGGCACTGT
TAATCATTTTCTCCCCTCGAGGTATTGTAGGTATGTTCTCTAGCATGAAGCAAAACATCCAACAAAAAAGAGATTTACGG
CAAAAACAACTAAAAAAAGAAGCGTAG

Upstream 100 bases:

>100_bases
CTATATCTCTAGTGATTATAAAGATATTATTGCTTTCGTTCTTCTGATCATTATTCTCTCTATCAAGCCTAATGGCATAT
TTGCTAAGGGGGAACACTAA

Downstream 100 bases:

>100_bases
AAAGGGGTGTATACATGTATATAGAAACAAAGGCATTATCCAAACAGTTTGGGGGATTAAGTGCCGTAAATAATGTTGAT
TTTTCAATAGATAAAGGGAA

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MIKNNTQKYIVIGLLMVAILFPLFTQNNYYIHVMTLSFIWMIAVYGLNILAGYTGYLSLAHAGFFAIGAYALGLLTVKAE
VGFWLALILAPIITTIIGFGIGLIALRTKEHFFAIYTLCVGYVIYLLIDKWDSLTEGVRGLMGIPLPSDIGPISFESNIA
QYYFILIVLLFSIFVIYRIVNSIVGKKLIAIRNSEQLALSLGISTKKNKLLAFVLSTFFAGLAGALYASFIRFLGPDIGS
TNITFDLLMYLIVGGIGTLSGPIIGTLLVVWLSQNLQFLQDYRMLIFGPLLALLIIFSPRGIVGMFSSMKQNIQQKRDLR
QKQLKKEA

Sequences:

>Translated_328_residues
MIKNNTQKYIVIGLLMVAILFPLFTQNNYYIHVMTLSFIWMIAVYGLNILAGYTGYLSLAHAGFFAIGAYALGLLTVKAE
VGFWLALILAPIITTIIGFGIGLIALRTKEHFFAIYTLCVGYVIYLLIDKWDSLTEGVRGLMGIPLPSDIGPISFESNIA
QYYFILIVLLFSIFVIYRIVNSIVGKKLIAIRNSEQLALSLGISTKKNKLLAFVLSTFFAGLAGALYASFIRFLGPDIGS
TNITFDLLMYLIVGGIGTLSGPIIGTLLVVWLSQNLQFLQDYRMLIFGPLLALLIIFSPRGIVGMFSSMKQNIQQKRDLR
QKQLKKEA
>Mature_328_residues
MIKNNTQKYIVIGLLMVAILFPLFTQNNYYIHVMTLSFIWMIAVYGLNILAGYTGYLSLAHAGFFAIGAYALGLLTVKAE
VGFWLALILAPIITTIIGFGIGLIALRTKEHFFAIYTLCVGYVIYLLIDKWDSLTEGVRGLMGIPLPSDIGPISFESNIA
QYYFILIVLLFSIFVIYRIVNSIVGKKLIAIRNSEQLALSLGISTKKNKLLAFVLSTFFAGLAGALYASFIRFLGPDIGS
TNITFDLLMYLIVGGIGTLSGPIIGTLLVVWLSQNLQFLQDYRMLIFGPLLALLIIFSPRGIVGMFSSMKQNIQQKRDLR
QKQLKKEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=298, Percent_Identity=29.5302013422819, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36410; Mature: 36410

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNNTQKYIVIGLLMVAILFPLFTQNNYYIHVMTLSFIWMIAVYGLNILAGYTGYLSLA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGFFAIGAYALGLLTVKAEVGFWLALILAPIITTIIGFGIGLIALRTKEHFFAIYTLCV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVIYLLIDKWDSLTEGVRGLMGIPLPSDIGPISFESNIAQYYFILIVLLFSIFVIYRIV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NSIVGKKLIAIRNSEQLALSLGISTKKNKLLAFVLSTFFAGLAGALYASFIRFLGPDIGS
HHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TNITFDLLMYLIVGGIGTLSGPIIGTLLVVWLSQNLQFLQDYRMLIFGPLLALLIIFSPR
CCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIVGMFSSMKQNIQQKRDLRQKQLKKEA
CHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKNNTQKYIVIGLLMVAILFPLFTQNNYYIHVMTLSFIWMIAVYGLNILAGYTGYLSLA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGFFAIGAYALGLLTVKAEVGFWLALILAPIITTIIGFGIGLIALRTKEHFFAIYTLCV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVIYLLIDKWDSLTEGVRGLMGIPLPSDIGPISFESNIAQYYFILIVLLFSIFVIYRIV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NSIVGKKLIAIRNSEQLALSLGISTKKNKLLAFVLSTFFAGLAGALYASFIRFLGPDIGS
HHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TNITFDLLMYLIVGGIGTLSGPIIGTLLVVWLSQNLQFLQDYRMLIFGPLLALLIIFSPR
CCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIVGMFSSMKQNIQQKRDLRQKQLKKEA
CHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]