Click here to switch to the map view.

The map label for this gene is tenA [H]

Identifier: 23098096

GI number: 23098096

Start: 676855

End: 677532

Strand: Reverse

Name: tenA [H]

Synonym: OB0641

Alternate gene names: 23098096

Gene position: 677532-676855 (Counterclockwise)

Preceding gene: 23098097

Following gene: 28563911

Centisome position: 18.66

GC content: 38.79

Gene sequence:

>678_bases
ATGCTTTTTTCAGATTCATTGCGTGAGGCAACAAAAGAAAGCTGGCAGTTAAGTTTAAATCATCCATTTGTACAAAAGGT
CGTAAGTGGTGATTTGCCACTAGAAACATTCAAAAATTATATTCTACAAGATATCTATTATTTGAAACATTACGGGAAGG
TGCATGCTTTTGCGGCTGCTCATTCAGATGACTTTCATATTGCCGCTAAACTGGCAGAAAAAGCAAAGAAGACCGCTGAA
GCAGAACTTACGGTGCATAAGGAGCACGCAGAAATTTTACAGATTACAGAAGCTGAAATCGAGAATTTCAAACCAGCACC
GACTGCATACGCGTATACATCACATTTATACCGCGCGTCGCTGTCCGGAAGTTTAGCTCAAATTGTAGCTGCAATGCTAC
CATGTTATTGGTTGTATGCAGATATTGGTTTGACCTACAAGGATGCGAAGCCAAAGGAAAAAATTTACCAGAACTGGTTG
AACACCTATGGCAGTGATTGGTTCCAAGAATCCACACAAGAAATGATTGATCTGCTCAATACTTTAGCTGAACAAGCAGG
AGAAGCAGAGAAGGAAAAAATCAAAACACAATTTATTATTGCGAAAGAATACGAACTTGCATTTTGGGAAATGTCCTATA
CATTTGAAACATGGCTTTCGGAAAAACAACAAATCTAG

Upstream 100 bases:

>100_bases
GGGATTTTCTTGGAAGATCAGCAGGATAAAAACGATTTAATGTATATTGTTAACGCAGTTGTAGAAGTAGGCTATTAAAC
AAAATTAAGGGGGATTTTCT

Downstream 100 bases:

>100_bases
CAGACTTTTAAATATACGGAATTGTAATAATAAGTGAACTACTTATAGATTGTGGTTAATATAATCAACCCATAACAGCT
TCGTGTAAAATGTACGTCAC

Product: transcriptional activator of extracellular enzyme

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLFSDSLREATKESWQLSLNHPFVQKVVSGDLPLETFKNYILQDIYYLKHYGKVHAFAAAHSDDFHIAAKLAEKAKKTAE
AELTVHKEHAEILQITEAEIENFKPAPTAYAYTSHLYRASLSGSLAQIVAAMLPCYWLYADIGLTYKDAKPKEKIYQNWL
NTYGSDWFQESTQEMIDLLNTLAEQAGEAEKEKIKTQFIIAKEYELAFWEMSYTFETWLSEKQQI

Sequences:

>Translated_225_residues
MLFSDSLREATKESWQLSLNHPFVQKVVSGDLPLETFKNYILQDIYYLKHYGKVHAFAAAHSDDFHIAAKLAEKAKKTAE
AELTVHKEHAEILQITEAEIENFKPAPTAYAYTSHLYRASLSGSLAQIVAAMLPCYWLYADIGLTYKDAKPKEKIYQNWL
NTYGSDWFQESTQEMIDLLNTLAEQAGEAEKEKIKTQFIIAKEYELAFWEMSYTFETWLSEKQQI
>Mature_225_residues
MLFSDSLREATKESWQLSLNHPFVQKVVSGDLPLETFKNYILQDIYYLKHYGKVHAFAAAHSDDFHIAAKLAEKAKKTAE
AELTVHKEHAEILQITEAEIENFKPAPTAYAYTSHLYRASLSGSLAQIVAAMLPCYWLYADIGLTYKDAKPKEKIYQNWL
NTYGSDWFQESTQEMIDLLNTLAEQAGEAEKEKIKTQFIIAKEYELAFWEMSYTFETWLSEKQQI

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=216, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324997, Length=213, Percent_Identity=25.8215962441315, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325378, Length=213, Percent_Identity=26.2910798122066, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 25981; Mature: 25981

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSDSLREATKESWQLSLNHPFVQKVVSGDLPLETFKNYILQDIYYLKHYGKVHAFAAA
CCCCHHHHHHHHHHHEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHC
HSDDFHIAAKLAEKAKKTAEAELTVHKEHAEILQITEAEIENFKPAPTAYAYTSHLYRAS
CCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LSGSLAQIVAAMLPCYWLYADIGLTYKDAKPKEKIYQNWLNTYGSDWFQESTQEMIDLLN
HCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TLAEQAGEAEKEKIKTQFIIAKEYELAFWEMSYTFETWLSEKQQI
HHHHHHCCHHHHHHHHHHEEEEHHEEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLFSDSLREATKESWQLSLNHPFVQKVVSGDLPLETFKNYILQDIYYLKHYGKVHAFAAA
CCCCHHHHHHHHHHHEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHC
HSDDFHIAAKLAEKAKKTAEAELTVHKEHAEILQITEAEIENFKPAPTAYAYTSHLYRAS
CCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LSGSLAQIVAAMLPCYWLYADIGLTYKDAKPKEKIYQNWLNTYGSDWFQESTQEMIDLLN
HCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TLAEQAGEAEKEKIKTQFIIAKEYELAFWEMSYTFETWLSEKQQI
HHHHHHCCHHHHHHHHHHEEEEHHEEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]