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The map label for this gene is pepT

Identifier: 23097986

GI number: 23097986

Start: 563650

End: 564879

Strand: Reverse

Name: pepT

Synonym: OB0531

Alternate gene names: 23097986

Gene position: 564879-563650 (Counterclockwise)

Preceding gene: 23097989

Following gene: 23097985

Centisome position: 15.56

GC content: 37.15

Gene sequence:

>1230_bases
ATGCAAAAACAACTTATCAATCGATTAATTACATATGCAAAAATAGACACACAATCTGACGGAGATTCAAGTACTACTCC
ATCAACACCTGGCCAGTGGGATTTAATTCACTATCTTAAAGATGAACTAAAAGAAATTGGCATGGAAGAAATCGAAGTCG
ATGAATATGGATATCTCATGGCTACATTACCAGCAAATTCGAAAAATGATATTCCGACGATCGGTTTTCTTGCCCACGTA
GATACTGCTACGGATTTTACTGGTAAGAACGTTAATCCGCAAATCATTGATTATAACGGCGGGGATATCGTATTAAATGA
AACGCTTCAAGTTGTGCTATCAACTAAGGAATTTCCAGAGCTTTCTTCCTATAAAGGACATACATTAATTACTACTGATG
GTACGACACTTTTAGGAGCTGACAATAAAGCGGGCATTGCTGAAATTATGACAGCTATGCAGTATCTCATCCAACATCCA
GAAATAGAGCATGGTACTATTCGTGTTGCTTTTACACCAGATGAAGAAATTGGACGTGGTCCGCATAAATTTGATGTATC
CCGTTTTAACGCTAAATACGCATATACGATCGACGGTGGTCCTTTAGGAGAACTTCAATATGAAAGTTTTAATGCTGCAG
CCGCAAAAGTCACCTTCACTGGGAACAGTGTTCACCCAGGTACTGCAAAGAATAAGATGGTCAATTCAGGAAAGATTGCT
GCTGAATTTATCTCGAAATTTCCAGAACAGGAAGCTCCCGAGCATACAGAGGGATATGAAGGGTTTTATCATTTAATATC
TATCGAAGGTGACGTAGAAACAACCAAAGTTTATTACATTATTCGCGATCATGATCGTAATAAGTTTGAAAATCGAAAAG
ATACGTTACGACAATTCGCTACTGATATACAACATAAATATGGAGAAAATGCTATTCACGTAGAAATGGAAGATCAGTAT
TACAATATGCGCGAGAAAATAGAGCCGGTTAAAGATATTGTCGATATAGCTAGTGAGGCTATGAAACAATTAGATATCGA
ACCTATTATTCAACCAGTACGTGGAGGAACAGACGGATCCCAACTCTCTTATATGGGATTACCAACTCCAAATATCTTTA
CCGGTGGAGAGAATTTCCACGGAAAATATGAGTATGTATCTGTTCAAAATATGGAAAAATCTACGAAAACGATTATTGGT
ATTGCTAAATTATTTGCTGAGAAATCATAA

Upstream 100 bases:

>100_bases
ATCTATTTTATTTTCTATCATATCATTTTATAAAATAATATACAGATATCTAAATAATCAATATATGATAAACTAATAGA
AAACGAAGAGGAGCTTCAAC

Downstream 100 bases:

>100_bases
GACTAATCAAATTCGTTTTGAGTCTGTGGTAATAACTGCCACTGGCTCAAAACTACATCCTTCATCTAATTTTCCACCTC
CCTTCTATTTGCTATAAATT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQKQLINRLITYAKIDTQSDGDSSTTPSTPGQWDLIHYLKDELKEIGMEEIEVDEYGYLMATLPANSKNDIPTIGFLAHV
DTATDFTGKNVNPQIIDYNGGDIVLNETLQVVLSTKEFPELSSYKGHTLITTDGTTLLGADNKAGIAEIMTAMQYLIQHP
EIEHGTIRVAFTPDEEIGRGPHKFDVSRFNAKYAYTIDGGPLGELQYESFNAAAAKVTFTGNSVHPGTAKNKMVNSGKIA
AEFISKFPEQEAPEHTEGYEGFYHLISIEGDVETTKVYYIIRDHDRNKFENRKDTLRQFATDIQHKYGENAIHVEMEDQY
YNMREKIEPVKDIVDIASEAMKQLDIEPIIQPVRGGTDGSQLSYMGLPTPNIFTGGENFHGKYEYVSVQNMEKSTKTIIG
IAKLFAEKS

Sequences:

>Translated_409_residues
MQKQLINRLITYAKIDTQSDGDSSTTPSTPGQWDLIHYLKDELKEIGMEEIEVDEYGYLMATLPANSKNDIPTIGFLAHV
DTATDFTGKNVNPQIIDYNGGDIVLNETLQVVLSTKEFPELSSYKGHTLITTDGTTLLGADNKAGIAEIMTAMQYLIQHP
EIEHGTIRVAFTPDEEIGRGPHKFDVSRFNAKYAYTIDGGPLGELQYESFNAAAAKVTFTGNSVHPGTAKNKMVNSGKIA
AEFISKFPEQEAPEHTEGYEGFYHLISIEGDVETTKVYYIIRDHDRNKFENRKDTLRQFATDIQHKYGENAIHVEMEDQY
YNMREKIEPVKDIVDIASEAMKQLDIEPIIQPVRGGTDGSQLSYMGLPTPNIFTGGENFHGKYEYVSVQNMEKSTKTIIG
IAKLFAEKS
>Mature_409_residues
MQKQLINRLITYAKIDTQSDGDSSTTPSTPGQWDLIHYLKDELKEIGMEEIEVDEYGYLMATLPANSKNDIPTIGFLAHV
DTATDFTGKNVNPQIIDYNGGDIVLNETLQVVLSTKEFPELSSYKGHTLITTDGTTLLGADNKAGIAEIMTAMQYLIQHP
EIEHGTIRVAFTPDEEIGRGPHKFDVSRFNAKYAYTIDGGPLGELQYESFNAAAAKVTFTGNSVHPGTAKNKMVNSGKIA
AEFISKFPEQEAPEHTEGYEGFYHLISIEGDVETTKVYYIIRDHDRNKFENRKDTLRQFATDIQHKYGENAIHVEMEDQY
YNMREKIEPVKDIVDIASEAMKQLDIEPIIQPVRGGTDGSQLSYMGLPTPNIFTGGENFHGKYEYVSVQNMEKSTKTIIG
IAKLFAEKS

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=407, Percent_Identity=53.8083538083538, Blast_Score=450, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_OCEIH (Q8CXN0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691452.1
- ProteinModelPortal:   Q8CXN0
- SMR:   Q8CXN0
- MEROPS:   M20.003
- GeneID:   1015806
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0531
- NMPDR:   fig|221109.1.peg.534
- HOGENOM:   HBG288638
- OMA:   HMDTAPD
- ProtClustDB:   PRK05469
- BioCyc:   OIHE221109:OB0531-MONOMER
- BRENDA:   3.4.11.4
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 45745; Mature: 45745

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 81-81 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQLINRLITYAKIDTQSDGDSSTTPSTPGQWDLIHYLKDELKEIGMEEIEVDEYGYLM
CHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEECCCCEEE
ATLPANSKNDIPTIGFLAHVDTATDFTGKNVNPQIIDYNGGDIVLNETLQVVLSTKEFPE
EEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCEEEECCHHHHHHCCCCCCC
LSSYKGHTLITTDGTTLLGADNKAGIAEIMTAMQYLIQHPEIEHGTIRVAFTPDEEIGRG
CCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCC
PHKFDVSRFNAKYAYTIDGGPLGELQYESFNAAAAKVTFTGNSVHPGTAKNKMVNSGKIA
CCEECHHHCCCEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHCCCCCCHHH
AEFISKFPEQEAPEHTEGYEGFYHLISIEGDVETTKVYYIIRDHDRNKFENRKDTLRQFA
HHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCHHHHHHHHHHHHH
TDIQHKYGENAIHVEMEDQYYNMREKIEPVKDIVDIASEAMKQLDIEPIIQPVRGGTDGS
HHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
QLSYMGLPTPNIFTGGENFHGKYEYVSVQNMEKSTKTIIGIAKLFAEKS
CEEEECCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKQLINRLITYAKIDTQSDGDSSTTPSTPGQWDLIHYLKDELKEIGMEEIEVDEYGYLM
CHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEECCCCEEE
ATLPANSKNDIPTIGFLAHVDTATDFTGKNVNPQIIDYNGGDIVLNETLQVVLSTKEFPE
EEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCEEEECCHHHHHHCCCCCCC
LSSYKGHTLITTDGTTLLGADNKAGIAEIMTAMQYLIQHPEIEHGTIRVAFTPDEEIGRG
CCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCC
PHKFDVSRFNAKYAYTIDGGPLGELQYESFNAAAAKVTFTGNSVHPGTAKNKMVNSGKIA
CCEECHHHCCCEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHCCCCCCHHH
AEFISKFPEQEAPEHTEGYEGFYHLISIEGDVETTKVYYIIRDHDRNKFENRKDTLRQFA
HHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCHHHHHHHHHHHHH
TDIQHKYGENAIHVEMEDQYYNMREKIEPVKDIVDIASEAMKQLDIEPIIQPVRGGTDGS
HHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
QLSYMGLPTPNIFTGGENFHGKYEYVSVQNMEKSTKTIIGIAKLFAEKS
CEEEECCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376