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The map label for this gene is trpE [H]

Identifier: 23097982

GI number: 23097982

Start: 558685

End: 560091

Strand: Reverse

Name: trpE [H]

Synonym: OB0527

Alternate gene names: 23097982

Gene position: 560091-558685 (Counterclockwise)

Preceding gene: 23097983

Following gene: 23097981

Centisome position: 15.43

GC content: 37.88

Gene sequence:

>1407_bases
ATGAGTATCCATTATGTAGTTGAGAAACAACCAATCGATCGATTATTACCTATTCAAGTACTAACCCAACTAACTAGCAC
AAAAAAATATTTATTAGAAAGCAGCTTTCCGCATGAAGAAAAAGGACGCTTTTCATTTCTAGGAATGAATCCATACCAAG
AAATTCTAGGATTCGATAACCGTACCAAGATTTGCAACTATACGAATGACGAAGATGAATGGGTAGAACAACATGCCTTA
CATTATATAAAACACCATCTACCTAAAGCCGAAATCGATATTTCCATTCCATTCTATGGTGGCGCAATCGGATATATCGG
CTACGATACGATACGTTCCTTTGAACACATCGGAGATCGATTACCCGATGAACGAAACATGCCAGACATACATCTGATGT
TATTTGAAGATACGATCATATTCGACCATGAACAAGATACCTGCTACCTCCTTGTGATTGATACGGATTCATCCACTTCT
CGAGATGATTTACAGGAACGTCTAGGCCAACTCAGAAAAATAATAAAGAAAGCAAGAGCAGCGACAGTCCCCATCCCAGC
GAGATATTCATTTACTCCAGAGATGGACCAAGACGTATTCGAAAAAAATGTAGCTACTGCAAAAAAACATATTGAAGACG
GAGATATTTTTCAAGTTGTATTGTCTCAGCGAATGAAGGCAAAGGTCGAAAATCCAGAAACCTTTTCCCTCTCCTTTTAT
GAACAACTGAGACAATCGAATCCTTCCCCTTACATGTTTTATATGGATTTTAATGATTACCTCATTCTTGGGGCTTCACC
AGAAAGTCTCATCGAAACAACTGGCAAGAAGATCATCACGAATCCAATAGCAGGTACAAGAAAAAGAGGAAGCACGAACG
AAATTGATCTCATGCTCACAGAGGAATTGCTTGCTGATGAAAAAGAAAGAGCGGAGCATCAAATGCTTGTAGATCTAAGT
AGAAATGACCTTGGAAAGGTATGTGAAATCGGAAGTATTGATATTCCGGTATATATGAAAATAGAAAAATATCAACATGT
CATGCATATGGTCTCTGAAGTCACTGGCATTTTAAAATCTAACTACTCTAGTATTGATGCTTTAATCTCTTGTTTACCAG
CTGGAACAGTATCCGGAGCACCCAAAATACGTGCAATGCAAATAATCAACGAGTTAGAGCAACACCAAAGAGGTCCTTAT
GGAGGAGGTATTGGATTTATTAACTTTCAACAAGATGTTCACATGGCTTTAGCTATTCGAACGGTGATCGTTAAAGATGA
CAAAGCATACTTACAAGCAGGAGCAGGAATCGTTTATGATTCCATACCTACAAGTGAGTATATTGAAACGCTAAATAAAG
CCAAATCATTTATGGAGGTGGACGATCATGATTTTATTAATCGATAA

Upstream 100 bases:

>100_bases
TGAGGTGGCACCGCGGTTCCTATCGTCCTCTATAAGCACACTAGCTGTGTTTATAGGGGACTTTTTTTATTCAATTACTT
TTAAATAAGGAGTCGAAGAC

Downstream 100 bases:

>100_bases
CTACGACTCATTTACGTATAACCTCTATCAATATATTTCTGAATTTAATAAGGAAGTCCGTGTTATTCGAAATGACGCAA
TAACACCTAATGAAATACGA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSIHYVVEKQPIDRLLPIQVLTQLTSTKKYLLESSFPHEEKGRFSFLGMNPYQEILGFDNRTKICNYTNDEDEWVEQHAL
HYIKHHLPKAEIDISIPFYGGAIGYIGYDTIRSFEHIGDRLPDERNMPDIHLMLFEDTIIFDHEQDTCYLLVIDTDSSTS
RDDLQERLGQLRKIIKKARAATVPIPARYSFTPEMDQDVFEKNVATAKKHIEDGDIFQVVLSQRMKAKVENPETFSLSFY
EQLRQSNPSPYMFYMDFNDYLILGASPESLIETTGKKIITNPIAGTRKRGSTNEIDLMLTEELLADEKERAEHQMLVDLS
RNDLGKVCEIGSIDIPVYMKIEKYQHVMHMVSEVTGILKSNYSSIDALISCLPAGTVSGAPKIRAMQIINELEQHQRGPY
GGGIGFINFQQDVHMALAIRTVIVKDDKAYLQAGAGIVYDSIPTSEYIETLNKAKSFMEVDDHDFINR

Sequences:

>Translated_468_residues
MSIHYVVEKQPIDRLLPIQVLTQLTSTKKYLLESSFPHEEKGRFSFLGMNPYQEILGFDNRTKICNYTNDEDEWVEQHAL
HYIKHHLPKAEIDISIPFYGGAIGYIGYDTIRSFEHIGDRLPDERNMPDIHLMLFEDTIIFDHEQDTCYLLVIDTDSSTS
RDDLQERLGQLRKIIKKARAATVPIPARYSFTPEMDQDVFEKNVATAKKHIEDGDIFQVVLSQRMKAKVENPETFSLSFY
EQLRQSNPSPYMFYMDFNDYLILGASPESLIETTGKKIITNPIAGTRKRGSTNEIDLMLTEELLADEKERAEHQMLVDLS
RNDLGKVCEIGSIDIPVYMKIEKYQHVMHMVSEVTGILKSNYSSIDALISCLPAGTVSGAPKIRAMQIINELEQHQRGPY
GGGIGFINFQQDVHMALAIRTVIVKDDKAYLQAGAGIVYDSIPTSEYIETLNKAKSFMEVDDHDFINR
>Mature_467_residues
SIHYVVEKQPIDRLLPIQVLTQLTSTKKYLLESSFPHEEKGRFSFLGMNPYQEILGFDNRTKICNYTNDEDEWVEQHALH
YIKHHLPKAEIDISIPFYGGAIGYIGYDTIRSFEHIGDRLPDERNMPDIHLMLFEDTIIFDHEQDTCYLLVIDTDSSTSR
DDLQERLGQLRKIIKKARAATVPIPARYSFTPEMDQDVFEKNVATAKKHIEDGDIFQVVLSQRMKAKVENPETFSLSFYE
QLRQSNPSPYMFYMDFNDYLILGASPESLIETTGKKIITNPIAGTRKRGSTNEIDLMLTEELLADEKERAEHQMLVDLSR
NDLGKVCEIGSIDIPVYMKIEKYQHVMHMVSEVTGILKSNYSSIDALISCLPAGTVSGAPKIRAMQIINELEQHQRGPYG
GGIGFINFQQDVHMALAIRTVIVKDDKAYLQAGAGIVYDSIPTSEYIETLNKAKSFMEVDDHDFINR

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=386, Percent_Identity=37.3056994818653, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788114, Length=433, Percent_Identity=28.8683602771363, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786809, Length=326, Percent_Identity=28.5276073619632, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082077, Length=263, Percent_Identity=27.3764258555133, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320935, Length=453, Percent_Identity=38.4105960264901, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6324361, Length=396, Percent_Identity=26.010101010101, Blast_Score=124, Evalue=3e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 53464; Mature: 53332

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHYVVEKQPIDRLLPIQVLTQLTSTKKYLLESSFPHEEKGRFSFLGMNPYQEILGFDN
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCC
RTKICNYTNDEDEWVEQHALHYIKHHLPKAEIDISIPFYGGAIGYIGYDTIRSFEHIGDR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHC
LPDERNMPDIHLMLFEDTIIFDHEQDTCYLLVIDTDSSTSRDDLQERLGQLRKIIKKARA
CCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ATVPIPARYSFTPEMDQDVFEKNVATAKKHIEDGDIFQVVLSQRMKAKVENPETFSLSFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHHHH
EQLRQSNPSPYMFYMDFNDYLILGASPESLIETTGKKIITNPIAGTRKRGSTNEIDLMLT
HHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEE
EELLADEKERAEHQMLVDLSRNDLGKVCEIGSIDIPVYMKIEKYQHVMHMVSEVTGILKS
HHHHCCHHHHHHHHHEEECCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHC
NYSSIDALISCLPAGTVSGAPKIRAMQIINELEQHQRGPYGGGIGFINFQQDVHMALAIR
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHEE
TVIVKDDKAYLQAGAGIVYDSIPTSEYIETLNKAKSFMEVDDHDFINR
EEEEECCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SIHYVVEKQPIDRLLPIQVLTQLTSTKKYLLESSFPHEEKGRFSFLGMNPYQEILGFDN
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCC
RTKICNYTNDEDEWVEQHALHYIKHHLPKAEIDISIPFYGGAIGYIGYDTIRSFEHIGDR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHC
LPDERNMPDIHLMLFEDTIIFDHEQDTCYLLVIDTDSSTSRDDLQERLGQLRKIIKKARA
CCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ATVPIPARYSFTPEMDQDVFEKNVATAKKHIEDGDIFQVVLSQRMKAKVENPETFSLSFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHHHH
EQLRQSNPSPYMFYMDFNDYLILGASPESLIETTGKKIITNPIAGTRKRGSTNEIDLMLT
HHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEE
EELLADEKERAEHQMLVDLSRNDLGKVCEIGSIDIPVYMKIEKYQHVMHMVSEVTGILKS
HHHHCCHHHHHHHHHEEECCCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHC
NYSSIDALISCLPAGTVSGAPKIRAMQIINELEQHQRGPYGGGIGFINFQQDVHMALAIR
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHEE
TVIVKDDKAYLQAGAGIVYDSIPTSEYIETLNKAKSFMEVDDHDFINR
EEEEECCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471 [H]