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The map label for this gene is exoA [H]

Identifier: 23097966

GI number: 23097966

Start: 545279

End: 546088

Strand: Reverse

Name: exoA [H]

Synonym: OB0511

Alternate gene names: 23097966

Gene position: 546088-545279 (Counterclockwise)

Preceding gene: 23097967

Following gene: 23097965

Centisome position: 15.04

GC content: 35.8

Gene sequence:

>810_bases
ATGCATTTTATATCGTGGAATGTAAACGGGATACGAGCTTGCGTTAGAAAAGGTTTTTTAGATTTTTTCCAAGAAATGGG
CGCAGATTTCTTTTGCATACAAGAATCAAAATTACAAGAAGGTCAAATTCAATTAGATTTAGATGGATATTATCAATATT
GGAACTATGCTGAACGAAAAGGTTATTCGGGTACAGCCATCTTCACCAAATGGGAGCCACTTCATGTTTTTTATGGGATC
GATTCCAATAACTACGAACCAGAAGGTAGAATTATTACCTTAGAATATGAATCATTTTATTTGGTCAATGTCTACACTCC
AAACTCCAAGCGTGATTTAACCCGATTAGAAGATCGCCTAGAATGGGAAGATCGTTTATTTGACTATTTGCAGAAATTAA
ATAAGAAAAAACCTCTAGTTTATTGTGGGGACTTGAATGTCGCTCATCATGAAATTGATCTACGAAACGATAAGACGAAT
CATGGAAATTCAGGGTTTACAAAAGAAGAAAGAAGTAAAATGTCGCGATTACTAGATGCCGGATTTGTCGATACACTTCG
TCATTTTCATCCAGAAGCAGACGACATCTTTACTTGGTGGTCTTATATGAAAACGATTCGTGAACGAAATATAGGATGGC
GAATCGATTATTTCATTGTCTCAGAGCAACTAATTCCAGCTCTTAAAAAATCGGAAGTACATTCTTCTGTTTTAGGAAGT
GATCATTGCCCTATTGGACTTGTCATAGATGAAAAACAATTACTACAACAGGGAGTTGGCGAAGATGAGAGGACTTATAC
ATCATATTGA

Upstream 100 bases:

>100_bases
TCCCGGGAAATGTTATTTTACAAAATAATAGTGATTTCAACAGGGAAAAAACGAATTATGATAAAATAACTAACAGATTA
ATGAGGAAGGAAGAACTGAT

Downstream 100 bases:

>100_bases
AATTTATGTTTCCAACTTACGACAATCTCAAGAATTCTGGGGATGGTTATTAGAAGATCTTGGATATGATTCTTACCAAA
AATGGGATAAAGGTCAAAGT

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MHFISWNVNGIRACVRKGFLDFFQEMGADFFCIQESKLQEGQIQLDLDGYYQYWNYAERKGYSGTAIFTKWEPLHVFYGI
DSNNYEPEGRIITLEYESFYLVNVYTPNSKRDLTRLEDRLEWEDRLFDYLQKLNKKKPLVYCGDLNVAHHEIDLRNDKTN
HGNSGFTKEERSKMSRLLDAGFVDTLRHFHPEADDIFTWWSYMKTIRERNIGWRIDYFIVSEQLIPALKKSEVHSSVLGS
DHCPIGLVIDEKQLLQQGVGEDERTYTSY

Sequences:

>Translated_269_residues
MHFISWNVNGIRACVRKGFLDFFQEMGADFFCIQESKLQEGQIQLDLDGYYQYWNYAERKGYSGTAIFTKWEPLHVFYGI
DSNNYEPEGRIITLEYESFYLVNVYTPNSKRDLTRLEDRLEWEDRLFDYLQKLNKKKPLVYCGDLNVAHHEIDLRNDKTN
HGNSGFTKEERSKMSRLLDAGFVDTLRHFHPEADDIFTWWSYMKTIRERNIGWRIDYFIVSEQLIPALKKSEVHSSVLGS
DHCPIGLVIDEKQLLQQGVGEDERTYTSY
>Mature_269_residues
MHFISWNVNGIRACVRKGFLDFFQEMGADFFCIQESKLQEGQIQLDLDGYYQYWNYAERKGYSGTAIFTKWEPLHVFYGI
DSNNYEPEGRIITLEYESFYLVNVYTPNSKRDLTRLEDRLEWEDRLFDYLQKLNKKKPLVYCGDLNVAHHEIDLRNDKTN
HGNSGFTKEERSKMSRLLDAGFVDTLRHFHPEADDIFTWWSYMKTIRERNIGWRIDYFIVSEQLIPALKKSEVHSSVLGS
DHCPIGLVIDEKQLLQQGVGEDERTYTSY

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=44.3137254901961, Blast_Score=240, Evalue=8e-64,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=44.3137254901961, Blast_Score=240, Evalue=8e-64,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=44.3137254901961, Blast_Score=240, Evalue=8e-64,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=28.8973384030418, Blast_Score=107, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=38.0392156862745, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71989539, Length=146, Percent_Identity=32.1917808219178, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=47.6, Blast_Score=257, Evalue=6e-69,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=47.6, Blast_Score=256, Evalue=9e-69,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31820; Mature: 31820

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFISWNVNGIRACVRKGFLDFFQEMGADFFCIQESKLQEGQIQLDLDGYYQYWNYAERK
CEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHC
GYSGTAIFTKWEPLHVFYGIDSNNYEPEGRIITLEYESFYLVNVYTPNSKRDLTRLEDRL
CCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHH
EWEDRLFDYLQKLNKKKPLVYCGDLNVAHHEIDLRNDKTNHGNSGFTKEERSKMSRLLDA
CHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFVDTLRHFHPEADDIFTWWSYMKTIRERNIGWRIDYFIVSEQLIPALKKSEVHSSVLGS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCC
DHCPIGLVIDEKQLLQQGVGEDERTYTSY
CCCCEEEEECHHHHHHCCCCCCCCEECCC
>Mature Secondary Structure
MHFISWNVNGIRACVRKGFLDFFQEMGADFFCIQESKLQEGQIQLDLDGYYQYWNYAERK
CEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHC
GYSGTAIFTKWEPLHVFYGIDSNNYEPEGRIITLEYESFYLVNVYTPNSKRDLTRLEDRL
CCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHH
EWEDRLFDYLQKLNKKKPLVYCGDLNVAHHEIDLRNDKTNHGNSGFTKEERSKMSRLLDA
CHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFVDTLRHFHPEADDIFTWWSYMKTIRERNIGWRIDYFIVSEQLIPALKKSEVHSSVLGS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCC
DHCPIGLVIDEKQLLQQGVGEDERTYTSY
CCCCEEEEECHHHHHHCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]