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The map label for this gene is mtnE [H]

Identifier: 23097875

GI number: 23097875

Start: 446124

End: 447287

Strand: Reverse

Name: mtnE [H]

Synonym: OB0420

Alternate gene names: 23097875

Gene position: 447287-446124 (Counterclockwise)

Preceding gene: 23097877

Following gene: 23097874

Centisome position: 12.32

GC content: 36.43

Gene sequence:

>1164_bases
ATGAAACAATCCAACCGATTAAAACAATTACCTCCACAATTTTTTGCATCTCTCGTACAAAAAGTAAATCAAGCCATTTC
TGAAGGAAGAGATGTTATTAATTTAGGGCAAGGTAATCCAGACCAACCTACTCCAGCTCATATTATAAAAGCTCTACAGT
CAGCTGTAGAAGATCCTATAACGCATAAGTATTCCCCTTTTCGCGGAACCGATGATTTTCGAAAAGCTGCTGCAACGTTT
TACAAACGAGAATATCAAGTAGATTTAGATCCTGATACTGAAATCGCTGTCTTATTCGGAAGTAAAATTGGACTTGTAGA
ACTTCCATTAGCACTGATGAACCCTAATGAATGGATGATGCTCCCTAATCCAGGATACCCCGATTATTTGTCGAGCATAC
CATTAGCTGATATTCAATATGATACGATGCCTCTTTTAGAAGAAAATAATTTTTTACCAAATTATGAACAACTTACGTTA
AGTCAAAAAGAACGGACTAAGTTATTGTATTTAAATTACCCAAATAATCCAACTGGAGCTACTGCAAATAATGCTTTCTT
TAATGAAACGGTGGCACTTGGTAAAAATCATGATATTGGAATTGTTCATGATTTTGCTTATGGAGCAATCGGTTTCAACG
AAGAAAAACCGATTAGTTTTCTACAAACAAAAGGGGCAAAAGAAGTAGGAATTGAGCTATATACGCTATCTAAAACGTAT
AATATGGCAGGTTGGCGCATCGGCTTTGCAGCTGGTAATAAAGAAATGATTGAAGCCATTAATATTTTACAAGATCATTT
ATTCGTCAGTGTCTTTCCTGCAATACAAAGAGCCGCTTCAGAAGCATTACTAAGTGATCAAAGCAGTGTACAAGATTTAG
TTACCCTATATCAAAAACGAAGAGATGCATTAATTACTGAATGCAAACGAATCGGTTGGGATATAAATGCGCCACAAGGC
TCTTTCTTTGCTTGGTTACCTGTACCAAATGGTTTCACTAGCGAAACTTTTGCTGATTATTTATTGAATGAAGTCGATGT
AGCGGTAGCAGCTGGTAATGGATTTGGAACTTTCGGTGAAGGATACATACGCGTAGGATTGCTTGTCGATGAACAACGTA
TTGTAGAAGCAATCAAGCGGATAGAAGGATTAAAACTATTTTAA

Upstream 100 bases:

>100_bases
TTTATGGTAAAAAATTCTTTGTTTAAAGGTAGACAGATACAAGGATTGAAGTTAATATTTATAGTAATTTGAACATTCCA
AAACAAAGGAGGATAACAAC

Downstream 100 bases:

>100_bases
ATCGACTTGAAAGGAAAGATTTACGTGAGTAAAGAGGATATACTTGAAATTGTTCATCAACTTAAAAAAGCACATCAATC
CAAACAACCCATTCCTTTTA

Product: transaminase

Products: N-succinyl-2-L-amino-6-oxoheptanedioate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKQSNRLKQLPPQFFASLVQKVNQAISEGRDVINLGQGNPDQPTPAHIIKALQSAVEDPITHKYSPFRGTDDFRKAAATF
YKREYQVDLDPDTEIAVLFGSKIGLVELPLALMNPNEWMMLPNPGYPDYLSSIPLADIQYDTMPLLEENNFLPNYEQLTL
SQKERTKLLYLNYPNNPTGATANNAFFNETVALGKNHDIGIVHDFAYGAIGFNEEKPISFLQTKGAKEVGIELYTLSKTY
NMAGWRIGFAAGNKEMIEAINILQDHLFVSVFPAIQRAASEALLSDQSSVQDLVTLYQKRRDALITECKRIGWDINAPQG
SFFAWLPVPNGFTSETFADYLLNEVDVAVAAGNGFGTFGEGYIRVGLLVDEQRIVEAIKRIEGLKLF

Sequences:

>Translated_387_residues
MKQSNRLKQLPPQFFASLVQKVNQAISEGRDVINLGQGNPDQPTPAHIIKALQSAVEDPITHKYSPFRGTDDFRKAAATF
YKREYQVDLDPDTEIAVLFGSKIGLVELPLALMNPNEWMMLPNPGYPDYLSSIPLADIQYDTMPLLEENNFLPNYEQLTL
SQKERTKLLYLNYPNNPTGATANNAFFNETVALGKNHDIGIVHDFAYGAIGFNEEKPISFLQTKGAKEVGIELYTLSKTY
NMAGWRIGFAAGNKEMIEAINILQDHLFVSVFPAIQRAASEALLSDQSSVQDLVTLYQKRRDALITECKRIGWDINAPQG
SFFAWLPVPNGFTSETFADYLLNEVDVAVAAGNGFGTFGEGYIRVGLLVDEQRIVEAIKRIEGLKLF
>Mature_387_residues
MKQSNRLKQLPPQFFASLVQKVNQAISEGRDVINLGQGNPDQPTPAHIIKALQSAVEDPITHKYSPFRGTDDFRKAAATF
YKREYQVDLDPDTEIAVLFGSKIGLVELPLALMNPNEWMMLPNPGYPDYLSSIPLADIQYDTMPLLEENNFLPNYEQLTL
SQKERTKLLYLNYPNNPTGATANNAFFNETVALGKNHDIGIVHDFAYGAIGFNEEKPISFLQTKGAKEVGIELYTLSKTY
NMAGWRIGFAAGNKEMIEAINILQDHLFVSVFPAIQRAASEALLSDQSSVQDLVTLYQKRRDALITECKRIGWDINAPQG
SFFAWLPVPNGFTSETFADYLLNEVDVAVAAGNGFGTFGEGYIRVGLLVDEQRIVEAIKRIEGLKLF

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable) [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily [H]

Homologues:

Organism=Homo sapiens, GI95147551, Length=319, Percent_Identity=24.4514106583072, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI169881279, Length=319, Percent_Identity=24.4514106583072, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI56713254, Length=308, Percent_Identity=22.7272727272727, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI56713256, Length=308, Percent_Identity=22.7272727272727, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4507369, Length=228, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788722, Length=386, Percent_Identity=36.2694300518135, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1786816, Length=377, Percent_Identity=24.6684350132626, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788627, Length=342, Percent_Identity=24.8538011695906, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71994472, Length=263, Percent_Identity=23.574144486692, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71994476, Length=263, Percent_Identity=23.574144486692, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17567369, Length=263, Percent_Identity=23.574144486692, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322401, Length=402, Percent_Identity=21.6417910447761, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28573069, Length=388, Percent_Identity=19.3298969072165, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646114, Length=388, Percent_Identity=19.3298969072165, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573067, Length=388, Percent_Identity=19.3298969072165, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573065, Length=388, Percent_Identity=19.3298969072165, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18859735, Length=346, Percent_Identity=23.9884393063584, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.17

Molecular weight: Translated: 43196; Mature: 43196

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSNRLKQLPPQFFASLVQKVNQAISEGRDVINLGQGNPDQPTPAHIIKALQSAVEDPI
CCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCC
THKYSPFRGTDDFRKAAATFYKREYQVDLDPDTEIAVLFGSKIGLVELPLALMNPNEWMM
CCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCEECCCEEECCCCEEE
LPNPGYPDYLSSIPLADIQYDTMPLLEENNFLPNYEQLTLSQKERTKLLYLNYPNNPTGA
ECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCC
TANNAFFNETVALGKNHDIGIVHDFAYGAIGFNEEKPISFLQTKGAKEVGIELYTLSKTY
CCCCCHHCCCEECCCCCCCEEEECCHHCCCCCCCCCCHHHHHHCCCHHHCEEEEEEECCC
NMAGWRIGFAAGNKEMIEAINILQDHLFVSVFPAIQRAASEALLSDQSSVQDLVTLYQKR
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RDALITECKRIGWDINAPQGSFFAWLPVPNGFTSETFADYLLNEVDVAVAAGNGFGTFGE
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCC
GYIRVGLLVDEQRIVEAIKRIEGLKLF
CEEEEEEEECHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKQSNRLKQLPPQFFASLVQKVNQAISEGRDVINLGQGNPDQPTPAHIIKALQSAVEDPI
CCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCC
THKYSPFRGTDDFRKAAATFYKREYQVDLDPDTEIAVLFGSKIGLVELPLALMNPNEWMM
CCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCEECCCEEECCCCEEE
LPNPGYPDYLSSIPLADIQYDTMPLLEENNFLPNYEQLTLSQKERTKLLYLNYPNNPTGA
ECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCC
TANNAFFNETVALGKNHDIGIVHDFAYGAIGFNEEKPISFLQTKGAKEVGIELYTLSKTY
CCCCCHHCCCEECCCCCCCEEEECCHHCCCCCCCCCCHHHHHHCCCHHHCEEEEEEECCC
NMAGWRIGFAAGNKEMIEAINILQDHLFVSVFPAIQRAASEALLSDQSSVQDLVTLYQKR
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RDALITECKRIGWDINAPQGSFFAWLPVPNGFTSETFADYLLNEVDVAVAAGNGFGTFGE
HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCC
GYIRVGLLVDEQRIVEAIKRIEGLKLF
CEEEEEEEECHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-succinyl-L-2,6-diaminoheptanedioate; 2-oxoglutarate

Specific reaction: N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]