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The map label for this gene is mhpD3 [H]

Identifier: 23097874

GI number: 23097874

Start: 445317

End: 446099

Strand: Reverse

Name: mhpD3 [H]

Synonym: OB0419

Alternate gene names: 23097874

Gene position: 446099-445317 (Counterclockwise)

Preceding gene: 23097875

Following gene: 23097860

Centisome position: 12.29

GC content: 36.53

Gene sequence:

>783_bases
GTGAGTAAAGAGGATATACTTGAAATTGTTCATCAACTTAAAAAAGCACATCAATCCAAACAACCCATTCCTTTTATTCG
AGATACGTTCAATTTAACAGAAGAGGAAGCTTATCACGTTCAGGAGTTATTTATTGAACATCAACTTGAAGAAAAAAATG
TACATATTTCAGGATATAAAGTTAGTATGACAAGTAAAGAGACACAAGCAATAGCGAGTACACATGAACCAGCATATGGC
ACATTGCTATCGCATCATGTATTAACTTCAGAAAGCAAGCTATCCAAATCGGAACTTTTTCAGCCTCTACTTGAACCTGA
AATAATCTTTATAGTAAACGAAGATTTAACACTAGACGCAGAAGAAACAGAGATTCTTCAGAAGACAAGTATTGCTGCTG
GAATAGAGATTCCTGATGCGCGCTATATTGATTGGTTCCCAAACTTTTCTCTCATCGACTTACTAAGTGATAATACTGCA
ACTGGACGAGTAGTCGTTGCTGAATCGGCACCTATATTAACCTTAGAACAGTTAGGAAACATTTCGTTAGAACTATATTA
TAACGGAGAGAAGATCAAAGAAGCATTTTCTTCCGCTGTATTAGACAATCCAATTTATGCTTTACGCTGGTTAAGCCAAA
AACTAGCGCTACATGGAACAACTCTAAAAAGAGGGATGATCATATCTTCTGGCACTTTTATTCCTCCAATTCCATTAGAG
ATTGGTCATTACGAAGCAGTATACTCCGGAGTCGGAAAAGCAGAAATAACGATCGTGAACTAA

Upstream 100 bases:

>100_bases
ACGCGTAGGATTGCTTGTCGATGAACAACGTATTGTAGAAGCAATCAAGCGGATAGAAGGATTAAAACTATTTTAAATCG
ACTTGAAAGGAAAGATTTAC

Downstream 100 bases:

>100_bases
ATAAATAAAACACCTCTAAGGATAGAGAGCATCTACCTTTAGGGGTGTTTTACTATACTTGGGCCGAACTTACGATTTAT
AATAACGATGTACGATAGCA

Product: 2-hydroxypenta-2,4-dienoate hydratase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase 3 [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSKEDILEIVHQLKKAHQSKQPIPFIRDTFNLTEEEAYHVQELFIEHQLEEKNVHISGYKVSMTSKETQAIASTHEPAYG
TLLSHHVLTSESKLSKSELFQPLLEPEIIFIVNEDLTLDAEETEILQKTSIAAGIEIPDARYIDWFPNFSLIDLLSDNTA
TGRVVVAESAPILTLEQLGNISLELYYNGEKIKEAFSSAVLDNPIYALRWLSQKLALHGTTLKRGMIISSGTFIPPIPLE
IGHYEAVYSGVGKAEITIVN

Sequences:

>Translated_260_residues
MSKEDILEIVHQLKKAHQSKQPIPFIRDTFNLTEEEAYHVQELFIEHQLEEKNVHISGYKVSMTSKETQAIASTHEPAYG
TLLSHHVLTSESKLSKSELFQPLLEPEIIFIVNEDLTLDAEETEILQKTSIAAGIEIPDARYIDWFPNFSLIDLLSDNTA
TGRVVVAESAPILTLEQLGNISLELYYNGEKIKEAFSSAVLDNPIYALRWLSQKLALHGTTLKRGMIISSGTFIPPIPLE
IGHYEAVYSGVGKAEITIVN
>Mature_259_residues
SKEDILEIVHQLKKAHQSKQPIPFIRDTFNLTEEEAYHVQELFIEHQLEEKNVHISGYKVSMTSKETQAIASTHEPAYGT
LLSHHVLTSESKLSKSELFQPLLEPEIIFIVNEDLTLDAEETEILQKTSIAAGIEIPDARYIDWFPNFSLIDLLSDNTAT
GRVVVAESAPILTLEQLGNISLELYYNGEKIKEAFSSAVLDNPIYALRWLSQKLALHGTTLKRGMIISSGTFIPPIPLEI
GHYEAVYSGVGKAEITIVN

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=261, Percent_Identity=26.0536398467433, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 29115; Mature: 28983

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEDILEIVHQLKKAHQSKQPIPFIRDTFNLTEEEAYHVQELFIEHQLEEKNVHISGYK
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
VSMTSKETQAIASTHEPAYGTLLSHHVLTSESKLSKSELFQPLLEPEIIFIVNEDLTLDA
EEECCHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCEEEEECCCEEECC
EETEILQKTSIAAGIEIPDARYIDWFPNFSLIDLLSDNTATGRVVVAESAPILTLEQLGN
HHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCEEEEECCCCEEEHHHCCC
ISLELYYNGEKIKEAFSSAVLDNPIYALRWLSQKLALHGTTLKRGMIISSGTFIPPIPLE
EEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCC
IGHYEAVYSGVGKAEITIVN
CCCHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
SKEDILEIVHQLKKAHQSKQPIPFIRDTFNLTEEEAYHVQELFIEHQLEEKNVHISGYK
CHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
VSMTSKETQAIASTHEPAYGTLLSHHVLTSESKLSKSELFQPLLEPEIIFIVNEDLTLDA
EEECCHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCEEEEECCCEEECC
EETEILQKTSIAAGIEIPDARYIDWFPNFSLIDLLSDNTATGRVVVAESAPILTLEQLGN
HHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCEEEEECCCCEEEHHHCCC
ISLELYYNGEKIKEAFSSAVLDNPIYALRWLSQKLALHGTTLKRGMIISSGTFIPPIPLE
EEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCC
IGHYEAVYSGVGKAEITIVN
CCCHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791097; 12823813 [H]