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The map label for this gene is amyX [H]

Identifier: 23097860

GI number: 23097860

Start: 429975

End: 432140

Strand: Reverse

Name: amyX [H]

Synonym: OB0405

Alternate gene names: 23097860

Gene position: 432140-429975 (Counterclockwise)

Preceding gene: 23097874

Following gene: 23097859

Centisome position: 11.9

GC content: 35.41

Gene sequence:

>2166_bases
ATGACTGATTCAATAATTGCTTGGTATGACGATGTACATCGATTAATCATTGAACATCCATCATCCAATTTTTTAAACAA
GCCAAATACTCCTGTACGTATTACAGAAAGATCAACAAATACTCATTATTACAACGCTAATATAAACCATCGTGATGAAA
AAGAGATAATCGTTGAATTAGATAACCCGCTCCCTATTGGAAGAGATTTAGTGCTTGTATGGAACGAATTAGAAATCCCT
ATATATACTCGTAAAATCACACGAACAAAGTGGTTTGAAGATAATTTTTTTACAAATCGAAAGTTAGGTGCGTTTTGTAA
TAACAAGTCCACTTCCTTTTCCATTTGGTCTCCAGTAGCAACTGCTGTACATTTACAGATAAAAAATAACTTCTATCTTT
TAGATAGGAATCAAGATGGAAGCTGGTCAATAAGCCTTGATGGAGACTTTCATCGCACGACCTATATATATCAAATTACG
ATCAATGATTTCACTCAGGAAGTTATCGATCCTTACGCAAAATCAGTCACTGCAAATAGTACTGAAGGAGTAATTATAGA
CCTTAATCGAACTAACGCTTTAAATCATCAACGCCCAAATAAAATTACTAAATTCCAAGATGCGATAATTTATGAATTAC
ATCTGAGAGACTTATCCACACATGAAAATAGTGGAATCAACAATAAAGGAAATTATCTCGGACTAACTGAATCAAATACC
GTAACCAATCTAGGATATTCAACTGGCAGGTGTTATATAAACGAACTCGGAATTACACATGTGCAATTACTACCTGTAAA
TGATTTTGCTAGAGTCGATGACCTGCATCCCAATAATTCATATAACTGGGGTTATGATCCATTATTTTTTCAAGCACCAG
AAGGAAGCTATTCATCCAACCCAACTAACCCCCTTTCACGAATAAATGAACTTAAATCTTGTATAGATTCCTGTCATGAG
GATAACCTATCCGTCATCTTGGATGTCGTATACAACCATGTATTCCAAATTGACCATTCCTCTTTTGAAAAAATGGTTCC
AGGTTATTTTTTTAGATATCACGACAATGGAGAATTAAGTAATGGTACGGGGGTAGGAAACGATTTTGCTAGTGAAAAAT
TAATGGCAAGAAAATTTATATTAGACACGATTGATTATTTTTTAAATGAATATAATGTAGATGGTTTTCGTTTTGACCTT
ATGGGAGCGATAGATGTGACTACTATGCAGGAAATACAAAAACGTTGTCAGAAAGAGAAAGAATTTATATTGCTGCTAGG
AGAAGGGTGGGATTTACCTACTGCGTTACCTGCGAATCAAAAATCCACACCAAATCAAGCACACGAATTAACAGGAATAG
CTTTCTTTAATGATTTTTTTCGTGATACCTTAAAAGGAAATCTATTTGCAGCTGATTCAAAAGGGTATGTGAATGGAGAA
GGTATGCATGTAGAACATCTACCGCATTTAGTGACCGGTTGTGTGTTAAGTAAATATGGTATACCCTTTGCTGAAAATCC
AGGTCAAATCGTTAATTATGTTGAATGCCATGACAATCACACGCTTTGGGATCGGTTATCTATTACCAATTCTCATTTCA
CCGTAGATGAACGAAAAAAAACACACCAACTGGCTACTGCTATAACAATCCTCAGCCAAGGCATTCCTTTTCTTCATGCG
GGGCAAGAGTGGTATCGGACAAAACACGGAGACGAAAACAGTTATCAATCTGGCGATAATATTAACCAACTCAATTGGAG
TCAACGAGAAACGGAAGAAGAATCGATTGCTTTTGTTCGTACATTGATTGAACTCCGAAAAAGATATCCGGTATTTCGTT
TACCAAGTGTTGAAGATATAAACAATTATGTTCATATCCTCCCTACCATTCATCCGGTTTTCGGATACACGCTTTTAGAC
AATGATGCAGATATTACCATCTATATCAATCCAACAAATACTTGCTATGATTTACAGTTACCTTCTTCTGCAAATTGGAA
AGTCGCTGTTAGTAATGAATTTATTAAAAGGAATGAACAAGTGGTTATTGGCGAACAAACAACACTACATCCCTATGAAA
TTCTCGTATTAGTAAAACAACGATCGCATCAGAAAGCTAAGAATCACGCGATAAAATCTTATGTATCACAGGAAAAAGGT
CAGTGA

Upstream 100 bases:

>100_bases
GCAAATACCTCGTCCAAACTTCATTATAGTTCGGATAAAAGTCCCCTCAGTTAGGGTATAACATATACTACATCAAACAT
CGGAATAAGGAGGAGCGCCA

Downstream 100 bases:

>100_bases
GAATCGAACAAAGTTAGTATAGATTTTCTTCTAGCTCATACTTGTTGTAAACGTATAACTCTACTCTTTCCCCTCCATCG
TTTAGAATAAATTGTATAAG

Product: pullulanase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTDSIIAWYDDVHRLIIEHPSSNFLNKPNTPVRITERSTNTHYYNANINHRDEKEIIVELDNPLPIGRDLVLVWNELEIP
IYTRKITRTKWFEDNFFTNRKLGAFCNNKSTSFSIWSPVATAVHLQIKNNFYLLDRNQDGSWSISLDGDFHRTTYIYQIT
INDFTQEVIDPYAKSVTANSTEGVIIDLNRTNALNHQRPNKITKFQDAIIYELHLRDLSTHENSGINNKGNYLGLTESNT
VTNLGYSTGRCYINELGITHVQLLPVNDFARVDDLHPNNSYNWGYDPLFFQAPEGSYSSNPTNPLSRINELKSCIDSCHE
DNLSVILDVVYNHVFQIDHSSFEKMVPGYFFRYHDNGELSNGTGVGNDFASEKLMARKFILDTIDYFLNEYNVDGFRFDL
MGAIDVTTMQEIQKRCQKEKEFILLLGEGWDLPTALPANQKSTPNQAHELTGIAFFNDFFRDTLKGNLFAADSKGYVNGE
GMHVEHLPHLVTGCVLSKYGIPFAENPGQIVNYVECHDNHTLWDRLSITNSHFTVDERKKTHQLATAITILSQGIPFLHA
GQEWYRTKHGDENSYQSGDNINQLNWSQRETEEESIAFVRTLIELRKRYPVFRLPSVEDINNYVHILPTIHPVFGYTLLD
NDADITIYINPTNTCYDLQLPSSANWKVAVSNEFIKRNEQVVIGEQTTLHPYEILVLVKQRSHQKAKNHAIKSYVSQEKG
Q

Sequences:

>Translated_721_residues
MTDSIIAWYDDVHRLIIEHPSSNFLNKPNTPVRITERSTNTHYYNANINHRDEKEIIVELDNPLPIGRDLVLVWNELEIP
IYTRKITRTKWFEDNFFTNRKLGAFCNNKSTSFSIWSPVATAVHLQIKNNFYLLDRNQDGSWSISLDGDFHRTTYIYQIT
INDFTQEVIDPYAKSVTANSTEGVIIDLNRTNALNHQRPNKITKFQDAIIYELHLRDLSTHENSGINNKGNYLGLTESNT
VTNLGYSTGRCYINELGITHVQLLPVNDFARVDDLHPNNSYNWGYDPLFFQAPEGSYSSNPTNPLSRINELKSCIDSCHE
DNLSVILDVVYNHVFQIDHSSFEKMVPGYFFRYHDNGELSNGTGVGNDFASEKLMARKFILDTIDYFLNEYNVDGFRFDL
MGAIDVTTMQEIQKRCQKEKEFILLLGEGWDLPTALPANQKSTPNQAHELTGIAFFNDFFRDTLKGNLFAADSKGYVNGE
GMHVEHLPHLVTGCVLSKYGIPFAENPGQIVNYVECHDNHTLWDRLSITNSHFTVDERKKTHQLATAITILSQGIPFLHA
GQEWYRTKHGDENSYQSGDNINQLNWSQRETEEESIAFVRTLIELRKRYPVFRLPSVEDINNYVHILPTIHPVFGYTLLD
NDADITIYINPTNTCYDLQLPSSANWKVAVSNEFIKRNEQVVIGEQTTLHPYEILVLVKQRSHQKAKNHAIKSYVSQEKG
Q
>Mature_720_residues
TDSIIAWYDDVHRLIIEHPSSNFLNKPNTPVRITERSTNTHYYNANINHRDEKEIIVELDNPLPIGRDLVLVWNELEIPI
YTRKITRTKWFEDNFFTNRKLGAFCNNKSTSFSIWSPVATAVHLQIKNNFYLLDRNQDGSWSISLDGDFHRTTYIYQITI
NDFTQEVIDPYAKSVTANSTEGVIIDLNRTNALNHQRPNKITKFQDAIIYELHLRDLSTHENSGINNKGNYLGLTESNTV
TNLGYSTGRCYINELGITHVQLLPVNDFARVDDLHPNNSYNWGYDPLFFQAPEGSYSSNPTNPLSRINELKSCIDSCHED
NLSVILDVVYNHVFQIDHSSFEKMVPGYFFRYHDNGELSNGTGVGNDFASEKLMARKFILDTIDYFLNEYNVDGFRFDLM
GAIDVTTMQEIQKRCQKEKEFILLLGEGWDLPTALPANQKSTPNQAHELTGIAFFNDFFRDTLKGNLFAADSKGYVNGEG
MHVEHLPHLVTGCVLSKYGIPFAENPGQIVNYVECHDNHTLWDRLSITNSHFTVDERKKTHQLATAITILSQGIPFLHAG
QEWYRTKHGDENSYQSGDNINQLNWSQRETEEESIAFVRTLIELRKRYPVFRLPSVEDINNYVHILPTIHPVFGYTLLDN
DADITIYINPTNTCYDLQLPSSANWKVAVSNEFIKRNEQVVIGEQTTLHPYEILVLVKQRSHQKAKNHAIKSYVSQEKGQ

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=457, Percent_Identity=26.2582056892779, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789839, Length=304, Percent_Identity=23.3552631578947, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 82839; Mature: 82707

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSIIAWYDDVHRLIIEHPSSNFLNKPNTPVRITERSTNTHYYNANINHRDEKEIIVEL
CCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEE
DNPLPIGRDLVLVWNELEIPIYTRKITRTKWFEDNFFTNRKLGAFCNNKSTSFSIWSPVA
CCCCCCCCEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCCEECCCCCCEEEECCCE
TAVHLQIKNNFYLLDRNQDGSWSISLDGDFHRTTYIYQITINDFTQEVIDPYAKSVTANS
EEEEEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCC
TEGVIIDLNRTNALNHQRPNKITKFQDAIIYELHLRDLSTHENSGINNKGNYLGLTESNT
CCCEEEEECCCCCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCC
VTNLGYSTGRCYINELGITHVQLLPVNDFARVDDLHPNNSYNWGYDPLFFQAPEGSYSSN
EEECCCCCCEEEEEECCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC
PTNPLSRINELKSCIDSCHEDNLSVILDVVYNHVFQIDHSSFEKMVPGYFFRYHDNGELS
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCHHHHHCCCCEEEEECCCCCCC
NGTGVGNDFASEKLMARKFILDTIDYFLNEYNVDGFRFDLMGAIDVTTMQEIQKRCQKEK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHCCCC
EFILLLGEGWDLPTALPANQKSTPNQAHELTGIAFFNDFFRDTLKGNLFAADSKGYVNGE
EEEEEEECCCCCCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHCCCEEEECCCCEECCC
GMHVEHLPHLVTGCVLSKYGIPFAENPGQIVNYVECHDNHTLWDRLSITNSHFTVDERKK
CEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEHEEEECCCEEEEHHHHH
THQLATAITILSQGIPFLHAGQEWYRTKHGDENSYQSGDNINQLNWSQRETEEESIAFVR
HHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHH
TLIELRKRYPVFRLPSVEDINNYVHILPTIHPVFGYTLLDNDADITIYINPTNTCYDLQL
HHHHHHHCCCEEECCCHHHCCCEEEEECCCCHHHEEEEECCCCEEEEEECCCCCEEEEEC
PSSANWKVAVSNEFIKRNEQVVIGEQTTLHPYEILVLVKQRSHQKAKNHAIKSYVSQEKG
CCCCCEEEEECCHHHHCCCEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCC
Q
C
>Mature Secondary Structure 
TDSIIAWYDDVHRLIIEHPSSNFLNKPNTPVRITERSTNTHYYNANINHRDEKEIIVEL
CCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEE
DNPLPIGRDLVLVWNELEIPIYTRKITRTKWFEDNFFTNRKLGAFCNNKSTSFSIWSPVA
CCCCCCCCEEEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCCEECCCCCCEEEECCCE
TAVHLQIKNNFYLLDRNQDGSWSISLDGDFHRTTYIYQITINDFTQEVIDPYAKSVTANS
EEEEEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCC
TEGVIIDLNRTNALNHQRPNKITKFQDAIIYELHLRDLSTHENSGINNKGNYLGLTESNT
CCCEEEEECCCCCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCC
VTNLGYSTGRCYINELGITHVQLLPVNDFARVDDLHPNNSYNWGYDPLFFQAPEGSYSSN
EEECCCCCCEEEEEECCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC
PTNPLSRINELKSCIDSCHEDNLSVILDVVYNHVFQIDHSSFEKMVPGYFFRYHDNGELS
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCHHHHHCCCCEEEEECCCCCCC
NGTGVGNDFASEKLMARKFILDTIDYFLNEYNVDGFRFDLMGAIDVTTMQEIQKRCQKEK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHCCCC
EFILLLGEGWDLPTALPANQKSTPNQAHELTGIAFFNDFFRDTLKGNLFAADSKGYVNGE
EEEEEEECCCCCCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHCCCEEEECCCCEECCC
GMHVEHLPHLVTGCVLSKYGIPFAENPGQIVNYVECHDNHTLWDRLSITNSHFTVDERKK
CEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEHEEEECCCEEEEHHHHH
THQLATAITILSQGIPFLHAGQEWYRTKHGDENSYQSGDNINQLNWSQRETEEESIAFVR
HHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHH
TLIELRKRYPVFRLPSVEDINNYVHILPTIHPVFGYTLLDNDADITIYINPTNTCYDLQL
HHHHHHHCCCEEECCCHHHCCCEEEEECCCCHHHEEEEECCCCEEEEEECCCCCEEEEEC
PSSANWKVAVSNEFIKRNEQVVIGEQTTLHPYEILVLVKQRSHQKAKNHAIKSYVSQEKG
CCCCCEEEEECCHHHHCCCEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]